University of Alaska Fairbanks
Contact Info:
UAF IAB Genomics Core Lab
PO Box 757000
2145 Tanana Dr., 207 West Ridge Research Building
Fairbanks, AK 99775
https://sites.google.com/alaska.edu/genomicscorelab/home
Grants and Identifiers:
RRID: RRID:SCR_021291
Other Citation Identifiers3P20GM103395-19S1
Instrumentation:
Agilent 4200 Tapestation Genetic Analyzer
An automated electrophoresis tool for DNA and RNA sample quality control. Fully automated sample processing ffor samples 1 to 96. [Product Link]
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BioTek Synergy H1 Hybrid Multi-Mode Microplate Reader
Configurable multi mode microplate reader. [Product Link]
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GridION Mk1
Benchtop sequencing and analysis device designed to run and analyse up to five individually addressable MinION or Flongle Flow Cells by Oxford Nanopore Technologies. Good for labs with multiple projects that need advantages of nanopore sequencing: simple library preparation, real-time analysis and new biological insights from long reads. [Product Link]
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(Equipment No Longer Active At this Facility)
Illumina MiniSeq Next Generation Sequencer
Illumina NGS technology performs broad range of targeted DNA and RNA applications. [Product Link]
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Illumina MiSeq System
Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]
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Thermo Fisher Qubit 2.0 Fluorometer Qubit 2.0
Invitrogen Qubit Flex Fluorometer can selectively, accurately measure the concentration of up to 8 samples of DNA, RNA or protein simultaneously. Compared to single sample microvolume fluorometers, using the Qubit Flex Fluorometer for multiple samples reduces your time to data by up to 50%, with time savings beginning with batches of as few as 8 samples. Combining the one-of-a-kind Qubit Flex Fluorometer and optimized Invitrogen Qubit reagents and tube strips offers a complete solution for DNA, RNA and protein quantification. [Product Link]
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Thermo Scientific Nanodrop 1000 Spectrophotometer
The Thermo Scientific NanoDrop™ 1000 is a spectrophotometer that performs measuring of 1 ul samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the NanoDrop 1000 Spectrophotometer has the capability to measure highly concentrated samples without dilution (50X higher concentration than the samples measured by a standard cuvette spectrophotometer). [Product Link]
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Other Equipment
Analytik-Jena qTOWER3 84 G (qPCR) ABI 7900HT (qPCR) Amersham Imager (AI680UV)
Services Provided:
Sequencing - Next Generation Sequencing (NGS)
Shared Instrumentation Oversight & Maintenance
Whole Genome Sequencing For Bacterial Isolates - ONT GridION
Relevant Publications:
Description:
The UAF IAB Genomics Core Lab provides sequencing services, instrument access and training, and education to researchers in the Institute of Arctic Biology and beyond. Our services include library preparation and sequencing with short read technology on the Illumina MiSeq, and long-read sequencing on the ONT GridION. We host a suite of instruments for DNA and RNA quantification and characterization which are available to IAB and other UAF researchers. We provide training on these instruments, as well as occasional workshops. The IAB Genomics Core Lab is located on the Troth Yeddha' campus of the University of Alaska, Fairbanks in Fairbanks, Alaska.
