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Molecular Research Core Facility

Idaho State University

Contact Info:

Lisa

640 Memorial Drive Stop 8007

Gale Life Sciences Building Room 461

Pocatello, ID 83209-8007

https://www.isu.edu/mrcf/

Grants and Identifiers:

RRID: RRID:SCR_012598

Instrumentation:

ABI 3100 Capillary Genetic Analyzer

Benchtop capillary genetic analyzer multicolor fluorescence based DNA analysis system with 16 capillaries. It can process 96 or 384 well plates. [Product Link]

RRID:SCR_018430

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Agilent 5200 Fragment Analyzer System

Fragment Analyzer System allows you to separate 12 samples in parallel in as little as 15 minutes. [Product Link]

RRID:SCR_019417

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Bio-Rad CFX96 Real-Time PCR Detection System

The CFX96 Touch System is a real-time PCR detection system that combines optical technology with temperature control. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, thermal cycler performance, unrivaled stand-alone functionality, and easy-to-use software, the CFX96 Touch System is designed to advance your qPCR. [Product Link]

RRID:SCR_018064

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Bio-Rad Chemidoc Gel Imaging System

Gel imaging system that enables visible light,RGB, far red, near infrared, FR, NIR, fluorescence and chemiluminescence detection. It visualizes proteins without gel staining. [Product Link]

RRID:SCR_019684

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BioTek Synergy HTX Multi-Mode Microplate Reader

Microplate reader that is used for UV-Vis absorbance and filter-based fluorescence and luminescence workflows. [Product Link]

RRID:SCR_019749

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Illumina MiSeq System

Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]

RRID:SCR_020134

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Leica RM2255 Automated Microtome

Leica RM2255 rotary microtome is designed for fully motorized sectioning of both paraffin- and the Leica RM2255 in routine and in histology. Its two-in-one design concept, which allows motorized and manual sectioning, provides reproducible high-quality sections. [Product Link]

RRID:SCR_020229

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Olympus FV1000 Confocal Microscope

Next generation imaging system designed for high resolution, confocal observation of both fixed and living cells.FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens.In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photoactivation. [Product Link]

RRID:SCR_020337

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Sorvall Legend RT Centrifuge

Legend RT is a general-purpose tabletop centrifuge for biotechnological and pharmaceutic research. .The user-friendly "QUIKset" control panel permits easy selection of speed, RCF value, run time and deceleration profile, as well as temperature of the Legend RT. You can switch from speed to RCF display and vice versa, with a touch of a button and even during a run [Product Link]

RRID:SCR_020520

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Other Equipment

Olympus Multiphoton Microscope Leica DM6B - Widefield Microscope Zeiss EM900 - Transmission Electron Microscope FACSCalibur Flow Cytometer Leica CM3050S - Cryostat Veriti Thermal Cyclers

Services Provided:

Capillary Electrophoresis

Cell Imaging

Confocal Microscopy

Data Analysis

Data Processing And Interpretation

DNA Analysis

DNA Isolation

Electron Microscopy

ELISA

Flow Cytometric Analysis

Genomics

Genotyping

Library Services

Metagenomics Analysis

Microbiome

Microscopy

Multi-color Flow Cytometry

Multiphoton Microscopy

Plate Reader

Real-time qPCR

RNA analysis

RNA Integrity

RNA Isolation

RNAseq

Sequencing - Capillary Sequencing

Sequencing - DNA Sequencing

Sequencing - Next Generation Sequencing (NGS)

Shared Instrumentation Oversight & Maintenance

Spectroscopy

Whole Genome Sequencing

Whole Plant Imaging

Whole Slide Imaging

Relevant Publications:

1.) Bolino M, Duman H, Avcı İ, Kayili HM, Petereit J, Zundel C, Salih B, Karav S, Frese SA (2024 Sep 18). Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes bioRxiv, (), 2024.09.18.613515. . PMCID: 11429724.


2.) Opoku R, Carrasco E, De Lay NR, Martin JE (2024 Sep 1). Calcium Rescues Streptococcus pneumoniae D39 ΔmntE Manganese-Sensitive Growth Phenotype Microorganisms, 12(9), 1810. . PMCID: 11434433.


3.) Flores Martinez KE, Bloszies CS, Bolino MJ, Henrick BM, Frese SA (2024 Jul 2). Hemp hull fiber and two constituent compounds, N-trans-caffeoyltyramine and N-trans-feruloyltyramine, shape the human gut microbiome in vitro Food Chemistry: X, 23(), 101611. . PMCID: 11304861.


4.) Heil JA, Simler-Williamson A, Striluk ML, Trawick D, Capezza R, DeFehr C, Osorio A, Finney B, Turner KG, Bittleston LS (2024 Jun 26). Weather and leaf age separately contribute to temporal shifts in phyllosphere community structure and composition. bioRxiv : the preprint server for biology, (), . . PMID: 38979227.


5.) Gignac PM, Valdez D, Morhardt AC, Lynch LM (2024 Jun 14). Buffered Lugol's Iodine Preserves DNA Fragment Lengths Integrative Organismal Biology, 6(1), obae017. . PMCID: 11182668.


6.) Miller AJ, Gass J, Jo MC, Bishop L, Petereit J, Woodhams DC, Voyles J (2023 Jun 12). Towards the generation of gnotobiotic larvae as a tool to investigate the influence of the microbiome on the development of the amphibian immune system Philosophical Transactions of the Royal Society B: Biological Sciences, 378(1882), 20220125. . PMCID: 10258664.


7.) Mika A, Luelling SE, Pavek A, Nartker C, Heyneman AL, Jones KB, Barrott JJ (2019 Mar 24). Epigenetic Changes at the <i>Birc5</i> Promoter Induced by YM155 in Synovial Sarcoma. Journal of clinical medicine, 8(3), . . ID: 30909651.


8.) Blay ES, Schwabedissen SG, Magnuson TS, Aho KA, Sheridan PP, Lohse KA (2017 Oct). Variation in Biological Soil Crust Bacterial Abundance and Diversity as a Function of Climate in Cold Steppe Ecosystems in the Intermountain West, USA. Microbial ecology, 74(3), 691-700. doi: 10.1007/s00248-017-0981-3. ID: 28409197.


9.) Hanson R, Evilia C, Gilmer J, Woods L, Black B, Flores R, Pfau JC (2016 Aug). Libby amphibole-induced mesothelial cell autoantibodies bind to surface plasminogen and alter collagen matrix remodeling. Physiological reports, 4(15), . . ID: 27519611.


10.) Ray AE, Connon SA, Sheridan PP, Gilbreath J, Shields M, Newby DT, Fujita Y, Magnuson TS (2010 Jun). Intragenomic heterogeneity of the 16S rRNA gene in strain UFO1 caused by a 100-bp insertion in helix 6. FEMS microbiology ecology, 72(3), 343-53. . ID: 20557571.


11.) LaPatra SE, Evilia C, Winston V (2008 Mar). Positively selected sites on the surface glycoprotein (G) of infectious hematopoietic necrosis virus. The Journal of general virology, 89(), 703-708. doi: 10.1099/vir.0.83451-0. ID: 18272761.


Description:

The ISU Molecular Research Core Facility (MRCF), provides fee-based services in a wide variety of molecular techniques. NGS library preparation, DNA/RNA extractions, ELISAs, PCR/qPCR, Microscopy and Flow Cytometry to name a few. We serve external and internal customers and are willing to discuss projects and help troubleshoot molecular assays with any researcher. Visit www.mrcf.isu.edu for more info!