Beth Israel Deaconess Medical Center
John M. Asara, Ph.D.
3 Blackfan Circle, CLS-425
Beth Israel Deaconess Medical Center
Boston, MA 02115
https://www.bidmcmassspec.org
RRID: RRID:SCR_017735
Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer
HFX is our newest addition to the proteomics resource. It offers the fastest scanning rate of our Orbitraps, which improves proteomic depth for discovery applications, and integrates well with targeted applications such as parallel reaction monitoring. [Product Link]
RRID:SCR_020555
AB/SCIEX 6500 QTRAP
hybrid triple quadruple mass spectrometer
Thermo Scientific Orbitrap Exploris 480
high resolution mass spectrometer
Thermo Orbitrap Velos Elite
AB/SCIEX 5500 QTRAP
Thermo QExactive Plus Orbitrap
Thermo QExactive HF Orbitrap
Biochemical Analysis
Computational - Bioinformatics
Data Analysis
Gel Chromatography
Lipidomics Profiling
Mass Spectrometry
Metabolomics
Metabolomics Platform
Protein Extraction/Purification
Protein Post-translational Modification (PTM) Identification
Proteomics
Sequencing - Capillary Sequencing
Sequencing - Protein Sequencing
Shared Instrumentation Oversight & Maintenance
Stable Isotope Tracing
Western Blot
1.) You H, Havey L, Li Z, Wang Y, Asara JM, Guo R (2025 Jan 31). Epstein-Barr virus-driven cardiolipin synthesis sustains metabolic remodeling during B cell transformation. Science advances, 11(5), eadr8837. . ID: 39879311.
2.) Heinken A, Asara JM, Gnanaguru G, Singh C (2025 Jan 9). Systemic regulation of retinal medium-chain fatty acid oxidation repletes TCA cycle flux in oxygen-induced retinopathy. Communications biology, 8(1), 25. . ID: 39789310.
3.) Tejero JD, Hesterberg RS, Drapela S, Ilter D, Raizada D, Lazure F, Kashfi H, Liu M, Silvane L, Avram D, Fernández-García J, Asara JM, Fendt SM, Cleveland JL, Gomes AP (2025 Jan). Methylmalonic acid induces metabolic abnormalities and exhaustion in CD8(+) T cells to suppress anti-tumor immunity. Oncogene, 44(2), 105-114. doi: 10.1038/s41388-024-03191-1. ID: 39472497.
4.) Hillis AL, Tamir T, Perry GE, Asara JM, Johnson JL, Yaron TM, Cantley LC, White FM, Toker A (2024 Nov 19). Parallel phosphoproteomics and metabolomics map the global metabolic tyrosine phosphoproteome. Proceedings of the National Academy of Sciences of the United States of America, 121(47), e2413837121. . ID: 39536083.
5.) Burton EM, Liang JH, Mitra B, Asara JM, Gewurz BE (2024 Nov 8). Epstein-Barr Virus Latent Membrane Protein 1 Subverts IMPDH pathways to drive B-cell oncometabolism. bioRxiv : the preprint server for biology, (), . . ID: 39574729.
6.) Yadav S, Graham A, Al Hammood F, Garbark C, Vasudevan D, Pandey U, Asara JM, Rajasundaram D, Parkhitko AA (2024 Nov). Unique tau- and synuclein-dependent metabolic reprogramming in neurons distinct from normal aging. Aging cell, 23(11), e14277. . ID: 39137949.
7.) Zarei M, Hajihassani O, Hue JJ, Loftus AW, Graor HJ, Nakazzi F, Naji P, Boutros CS, Uppin V, Vaziri-Gohar A, Shalaby AS, Asara JM, Rothermel LD, Brody JR, Winter JM (2024 Sep 16). IDH1 Inhibition Potentiates Chemotherapy Efficacy in Pancreatic Cancer. Cancer research, 84(18), 3072-3085. . ID: 38843355.
8.) Nagesh PT, Cho Y, Zhuang Y, Babuta M, Ortega-Ribera M, Joshi R, Brezani V, Patel A, Datta AA, Brezani V, Hsieh YC, Ramos A, Mehta J, Copeland C, Kanata E, Jiang ZG, Vlachos I, Asara J, Szabo G (2024 Aug 7). In vivo Bruton Science translational medicine, 16(759), eadg1915. doi: 10.1126/scitranslmed.adg1915. ID: 39110779.
9.) Tripp BA, Dillon ST, Yuan M, Asara JM, Vasunilashorn SM, Fong TG, Inouye SK, Ngo LH, Marcantonio ER, Xie Z, Libermann TA, Otu HH (2024 Jul 30). Integrated Multi-Omics Analysis of Cerebrospinal Fluid in Postoperative Delirium. Biomolecules, 14(8), . . ID: 39199312.
10.) Raines NH, Leone DA, Amador JJ, Lopez-Pilarte D, Ramírez-Rubio O, Delgado IS, Francey LJ, Leibler JH, Asara JM, Scammell MK, Parikh SM, Brooks DR, Friedman DJ (2024 Jul). Derangement in Nicotinamide Adenine Dinucleotide Metabolism is Observed During Acute Kidney Injury Among Male Agricultural Workers at Risk for Mesoamerican Nephropathy. Kidney international reports, 9(7), 2250-2259. . ID: 39081728.
11.) Gewurz B, Guo R, Lim M, Shah H, Paulo J, Zhang Y, Yang H, Wang LW, Strebinger D, Smith N, Li M, Leong M, Lutchenkov M, Liang JH, Li Z, Wang Y, Puri R, Melnick A, Green M, Asara J, Papathanassiu A, Gygi S, Mootha V (2024 May 30). Multi-omic Analysis of Human B-cell Activation Reveals a Key Lysosomal BCAT1 Role in mTOR Hyperactivation by B-cell receptor and TLR9. Research square, (), . . ID: 38854072.
12.) Moon SJ, Hu Y, Dzieciatkowska M, Kim AR, Chen PL, Asara JM, D (2024 Apr 28). Identification of high sugar diet-induced dysregulated metabolic pathways in muscle using tissue-specific metabolic models in Drosophila. bioRxiv : the preprint server for biology, (), . . ID: 38712132.
13.) Wang Y, Guo R, Piedras BI, Tang HY, Asara JM, Tempera I, Lieberman PM, Gewurz BE (2024 Apr 24). The CTLH Ubiquitin Ligase Substrates ZMYND19 and MKLN1 Negatively Regulate mTORC1 at the Lysosomal Membrane. Research square, (), . . ID: 38746323.
14.) You H, Havey L, Li Z, Asara J, Guo R (2024 Apr 8). Epstein-Barr-Virus-Driven Cardiolipin Synthesis Sustains Metabolic Remodeling During B-cell Lymphomagenesis. Research square, (), . . ID: 38659762.
15.) Deritei D, Inuzuka H, Castaldi PJ, Yun JH, Xu Z, Anamika WJ, Asara JM, Guo F, Zhou X, Glass K, Wei W, Silverman EK (2024 Apr 1). HHIP protein interactions in lung cells provide insight into COPD pathogenesis. bioRxiv : the preprint server for biology, (), . . ID: 38617310.
16.) Sahu U, Villa E, Reczek CR, Zhao Z, O (2024 Mar 29). Pyrimidines maintain mitochondrial pyruvate oxidation to support de novo lipogenesis. Science (New York, N.Y.), 383(6690), 1484-1492. . ID: 38547260.
17.) Yang Q, Cai Y, Ma Q, Xiong A, Xu P, Zhang Z, Xu J, Zhou Y, Liu Z, Zhao D, Asara J, Li W, Shi H, Caldwell RB, Sodhi A, Huo Y (2024 Mar 6). Inactivation of adenosine receptor 2A suppresses endothelial-to-mesenchymal transition and inhibits subretinal fibrosis in mice. Science translational medicine, 16(737), eadk3868. . ID: 38446902.
18.) Hu AJ, Li W, Dinh C, Zhang Y, Hu JK, Daniele SG, Hou X, Yang Z, Asara JM, Hu GF, Farmer SR, Hu MG (2024 Feb 5). CDK6 inhibits de novo lipogenesis in white adipose tissues but not in the liver. Nature communications, 15(1), 1091. . ID: 38316780.
19.) Arai S, Gao Y, Yu Z, Xie L, Wang L, Zhang T, Nouri M, Chen S, Asara JM, Balk SP (2024 Jan 5). A carboxy-terminal ubiquitylation site regulates androgen receptor activity. Communications biology, 7(1), 25. . ID: 38182874.
20.) Yang Q, Huo E, Cai Y, Zhang Z, Dong C, Asara JM, Shi H, Wei Q (2023). Myeloid PFKFB3-mediated glycolysis promotes kidney fibrosis. Frontiers in immunology, 14(), 1259434. . ID: 38035106.
21.) Jiang D, Sun W, Wu T, Zou M, Vasamsetti SB, Zhang X, Zhao Y, Phillippi JA, Sawalha AH, Tavakoli S, Dutta P, Florentin J, Chan SY, Tollison TS, Di Wu, Cui J, Huntress I, Peng X, Finkel T, Li G (2022 Feb). Post-GWAS functional analysis identifies CUX1 as a regulator of p16(INK4a) and cellular senescence. Nature aging, 2(2), 140-154. . PMID: 37117763.
22.) Zhao Y, Wu D, Jiang D, Zhang X, Wu T, Cui J, Qian M, Zhao J, Oesterreich S, Sun W, Finkel T, Li G (2020 Jul 3). A sequential methodology for the rapid identification and characterization of breast cancer-associated functional SNPs. Nature communications, 11(1), 3340. . PMID: 32620845.
ABOUT US: The mass spectrometry, metabolomics and proteomics core at Beth Israel Deaconess Medical Center was established in 2004 led by Dr. John Asara and continues to grow rapidly. We provide excellent proteomics, metabolomics and lipidomics services with low prices and fast turnaround times. We have cutting edge equipment operated at optimal sensitivity and work and publish with world class researchers. We also maintain an active internal cancer proteomics and metabolomics/lipidomics research program with collaborative efforts. Our expertise is in identifying and quantifying protein modifications and dynamic protein-protein interactions in addition to polar metabolomics profiling, lipidomics profiling and 13C/15N metabolic flux analysis. We accept samples from anywhere in the world and look forward to helping you and progressing your research to the next level.
