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CU-Auditory & Vestibular Technologies (AVT) Core Facility

Creighton University

Contact Info:

Mike Nichols

2500 California Plaza

Creighton University

Omaha, NE 68178

https://www.creighton.edu/medicine/departments/biomedical-sciences/translational-hearing-center/cobre-translational-hearing-center/technology

Grants and Identifiers:

RRID: RRID:SCR_023866

Other Citation Identifiers

5P20GM139762-05

Instrumentation:

10X Genomics Chromium Plus Genetic Analyzer

Genetic analyzer, referred to as Chromium Controller for combining large partition numbers with a diverse barcode library to generate >100,000 barcode-containing partitions. [Product Link]

RRID:SCR_019326

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Agilent Seahorse XFe24 Analyzer

Seahorse XFe24 Analyzer measures OCR and ECAR of live cells in 24 well plate format. [Product Link]

RRID:SCR_019539

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Bio-Rad Chemidoc Gel Imaging System

Gel imaging system that enables visible light,RGB, far red, near infrared, FR, NIR, fluorescence and chemiluminescence detection. It visualizes proteins without gel staining. [Product Link]

RRID:SCR_019684

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Bruker Ultraflex III MALDI TOF/TOF Mass Spectrometer

MALDI (Matrix Assisted Laser Desorption/Ionization) tandem mass spectrometer specially designed for automated MS and MS/MS high throughput identification of proteins and peptides. Tandem Mass Spectrometry is a technique that utilizes more than one mass selective stage in a mass spectrometer. [Product Link]

RRID:SCR_019799

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Life Technologies QuantStudio 3 Real Time PCR System

Applied Biosystems QuantStudio 3 Real-Time PCR System is designed for users who need an , easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is best for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 3 system provides access to your data wherever and whenever you want. Using OptiFlex technology (featuring 4 coupled channels and white LED) and featuring three independent Veriflex temperature zones, the QuantStudio 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications. [Product Link]

RRID:SCR_020238

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Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

Identify and quantify more proteins, peptides, lipids, glycans and small molecules accurately and in less time with the Thermo Scientific Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer. The Q Exactive HF system combines a state-of-the-art segmented quadrupole for high-performance precursor ion selection with a high-resolution, accurate-mass (HR/AM) ultra-high-field Orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. Identify, quantify and confirm in a single analysis with a single instrument with the Q Exactive HF mass spectrometer. [Product Link]

RRID:SCR_020558

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Nikon AXR Laser Scanning Microscope with NSPARC

Applied Biosystems QuantStudio 3 Real-Time PCR System

Axon Instruments Axopatch 200B intergrating patch clamp amplifier

Patch clamp amplifier for recording cochlear potentials (CM, CAP, EP) from small mammals and microphonic response from zebrafish.

Bio-Techne Jess Automated Western Blot System

Capillary-based immunoassays and fully automated Western blot analysis

ISCAN Inc. ETL-200

eye-tracking system for VOR/OKR

MILLIPLEX LUMINEX L200 ANALYZER

Multiplex xMAP bead technology that allows for the simultaneous analysis of a broad selection of analytes (e.g. cytokines, hormones and adipokines) across a wide range of disease states, including metabolic disease, inflammation, neurodegenerative disease, toxicity, cancer and more.

(Equipment No Longer Active At this Facility)

Nikon Nikon AXR Laser Scanning Confocal Microscope with NSPARC

PPI Viewpoint

fast behavior system for high speed applications for zebrafish

ThermoFisher Thermo Vanquish Flex Binary UHPLC

A biocompatible quaternary UHPLC system for absolute quantitation of small molecules, lipidomics, and metabolomics

Tucker Davis Technologies TDT RZ6

High-frequency auditory signal processing

Viewpoint ZebraBox

Observation chamber for zebrafish behavioral studies

Waters ACQUITY

M-Class Chromatography System provides nanoflow technology with the straightforward setup found in an analytical-scale UPLC system

Zeiss 700 LSM Inverted Confocal Microscope

Zeiss 980 LSM Confocal Microscope with Airyscan 2

(Equipment No Longer Active At this Facility)

Zeiss PALM Combisystem

Widefield platform for micromanipulatino and microdissection of samples

(Equipment No Longer Active At this Facility)

Zeiss PALM Combisystem

Services Provided:

Analysis Workstations

Auditory & Vestibular Testing

Confocal Microscopy

Confocal/ Super Resolution Microscope

Consultation

Data Analysis And Interpretation

Data Processing And Interpretation

Differential Interference Contrast Microscopy

Experimental Design

Image Aquisition

Image Processing And Analysis

Laser Capture Microdissection

Live Cell Imaging

Mass Spectrometry

Mass Spectrometry - Proteins And Peptides

Microscopy

Real-time QPCR

Training

Zebrafish Behavioral Studies

Relevant Publications:

1.) Zallocchi M, Vijayakumar S, Fleegel J, Batalkina L, Brunette KE, Shukal D, Chen Z, Devuyst O, Liu H, He DZZ, Imami AS, Hamoud AA, McCullumsmith R, Conda-Sheridan M, De Campos LJ, Zuo J (2024 Aug 7). Piplartine attenuates aminoglycoside-induced TRPV1 activity and protects from hearing loss in mice. Science translational medicine, 16(759), eadn2140. . ID: 39110778.


2.) Sailor-Longsworth EJ, Lutze RD, Ingersoll MA, Kelmann RG, Ly K, Currier D, Chen T, Zuo J, Teitz T (2024 Aug). Oseltamivir (Tamiflu), a commonly prescribed antiviral drug, mitigates hearing loss in mice. Clinical and translational medicine, 14(8), e1803. . PMID: 39133201.


3.) Vijayakumar S, DiGuiseppi JA, Dabestani PJ, Ryan WG, Quevedo RV, Li Y, Diers J, Tu S, Fleegel J, Nguyen C, Rhoda LM, Imami AS, Hamoud AA, Lovas S, McCullumsmith RE, Zallocchi M, Zuo J (2024 Jun 21). In silico transcriptome screens identify epidermal growth factor receptor inhibitors as therapeutics for noise-induced hearing loss. Science advances, 10(25), eadk2299. . ID: 38896614.


4.) Lutze RD, Ingersoll MA, Thotam A, Joseph A, Fernandes J, Teitz T (2024 Jun 7). ERK1/2 Inhibition via the Oral Administration of Tizaterkib Alleviates Noise-Induced Hearing Loss While Tempering down the Immune Response International Journal of Molecular Sciences, 25(12), 6305. . PMCID: 11204379.


5.) Lutze RD, Ingersoll MA, Kelmann RG, Teitz T (2024 Jun 5). Trametinib, a MEK1/2 Inhibitor, Protects Mice from Cisplatin- and Noise-Induced Hearing Loss Pharmaceuticals, 17(6), 735. . PMCID: 11206450.


6.) Lutze RD, Ingersoll MA, Kelmann RG, Teitz T (2024 May 21). FDA-Approved MEK1/2 Inhibitor, Trametinib, Protects Mice from Cisplatin and Noise-Induced Hearing Loss bioRxiv, (), 2024.05.20.595056. . PMCID: 11142120.


7.) Sailor-Longsworth E, Lutze RD, Ingersoll MA, Kelmann RG, Ly K, Currier D, Chen T, Zuo J, Teitz T (2024 May 8). Oseltamivir (Tamiflu), a Commonly Prescribed Antiviral Drug, Mitigates Hearing Loss in Mice bioRxiv, (), 2024.05.06.592815. . PMCID: 11100672.


8.) Ingersoll MA, Lutze RD, Kelmann RG, Kresock DF, Marsh JD, Quevedo RV, Zuo J, Teitz T (2024 May 1). KSR1 Knockout Mouse Model Demonstrates MAPK Pathway's Key Role in Cisplatin- and Noise-induced Hearing Loss. The Journal of neuroscience : the official journal of the Society for Neuroscience, 44(18), . . PMID: 38548338.


9.) Ingersoll MA, Lutze RD, Pushpan CK, Kelmann RG, Liu H, May MT, Hunter WJ, He DZ, Teitz T (2023 Dec 22). Dabrafenib protects from cisplatin-induced hearing loss in a clinically relevant mouse model JCI Insight, 8(24), e171140. . PMCID: 10807719.


10.) Ingersoll MA, Lutze RD, Kelmann RG, Kresock DF, Marsh JD, Quevedo RV, Zuo J, Teitz T (2023 Nov 13). KSR1 knockout mouse model demonstrates MAPK pathway’s key role in cisplatin- and noise-induced hearing loss bioRxiv, (), 2023.11.08.566316. . PMCID: 10680565.


11.) Lutze RD, Ingersoll MA, Thotam A, Joseph A, Fernandes J, Teitz T (2023 Oct 20). ERK1/2 Inhibition Alleviates Noise-Induced Hearing Loss While Tempering Down the Immune Response. bioRxiv : the preprint server for biology, (), . . PMID: 37905140.


12.) Manickam V, Gawande DY, Stothert AR, Clayman AC, Batalkina L, Warchol ME, Ohlemiller KK, Kaur T (2023 Mar 22). Macrophages Promote Repair of Inner Hair Cell Ribbon Synapses following Noise-Induced Cochlear Synaptopathy. The Journal of neuroscience : the official journal of the Society for Neuroscience, 43(12), 2075-2089. . ID: 36810227.


13.) Madison J, Wilhelm K, Meehan DT, Delimont D, Samuelson G, Cosgrove D (2022 Sep). Glomerular basement membrane deposition of collagen α1(III) in Alport glomeruli by mesangial filopodia injures podocytes via aberrant signaling through DDR1 and integrin α2β1. The Journal of pathology, 258(1), 26-37. . ID: 35607980.


14.) Liu H, Giffen KP, Chen L, Henderson HJ, Cao TA, Kozeny GA, Beisel KW, Li Y, He DZ (2022 Apr 12). Molecular and cytological profiling of biological aging of mouse cochlear inner and outer hair cells. Cell reports, 39(2), 110665. . ID: 35417713.


15.) Giffen KP, Li Y, Liu H, Zhao XC, Zhang CJ, Shen RJ, Wang T, Janesick A, Chen BB, Gong SS, Kachar B, Jin ZB, He DZ (2022 Apr 8). Mutation of SLC7A14 causes auditory neuropathy and retinitis pigmentosa mediated by lysosomal dysfunction. Science advances, 8(14), eabk0942. . ID: 35394837.


16.) Hati S, Zallocchi M, Hazlitt R, Li Y, Vijayakumar S, Min J, Rankovic Z, Lovas S, Zuo J (2021 Dec 15). AZD5438-PROTAC: A selective CDK2 degrader that protects against cisplatin- and noise-induced hearing loss. European journal of medicinal chemistry, 226(), 113849. . ID: 34560429.


17.) Urbanek ME, Zuo J (2021 Sep 13). Genetic predisposition to tinnitus in the UK Biobank population. Scientific reports, 11(1), 18150. . ID: 34518561.


18.) Zallocchi M, Hati S, Xu Z, Hausman W, Liu H, He DZ, Zuo J (2021 Mar 8). Characterization of quinoxaline derivatives for protection against iatrogenically induced hearing loss. JCI insight, 6(5), . . ID: 33476306.


19.) Xu Z, Rai V, Zuo J (2021). TUB and ZNF532 Promote the Atoh1-Mediated Hair Cell Regeneration in Mouse Cochleae. Frontiers in cellular neuroscience, 15(), 759223. . ID: 34819838.


Description:

The Auditory & Vestibular Technology (AVT) Core was established to provide infrastructure to support Research Project Leaders and principle investigators associated with the Dr. Richard J. Bellucci Translational Hearing Center to conduct auditory and vestibular research across the full range of experimental model systems, from single molecule analysis to whole organism models. We also offer access to our services and instruments to outside users.