South Dakota State University
1390 College Ave
Rm 047
Brookings, SD 57007
https://www.sdstate.edu/biology-and-microbiology/functional-genomics-core-facility
RRID: RRID:SCR_023786
Life Technologies QuantStudio 6 Real Time PCR System
QuantStudio 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 96-well block in this case); other block types may be purchased separately. For a system that also supports TaqMan array cards, as well as automation, please see our QuantStudio 7 Flex system. The QuantStudio 6 Flex system can also be upgraded to a QuantStudio 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio 12K Flex system, which also accommodates TaqMan OpenArray plates. [Product Link]
RRID:SCR_020239
Nanodrop 2000c Spectrophotometer
Thermo Scientific NanoDrop 2000 and 2000c are full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, Protein and more. The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure sample volumes as small as 0.5?L. [Product Link]
RRID:SCR_020309
Olympus IX70 Inverted Fluorescence Tissue Culture Microscope
Inverted fluorescence tissue culture microscope capable of imaging specimens in brightfield, darkfield, phase contrast, hoffman modulation contrast, fluorescence, and differential interference contrast modes. [Product Link]
RRID:SCR_018604
Olympus Bx53 upright microscope
Olympus FV1200 confocal microscope
Biolayer Interferometry (BLI)
CDNA Synthesis
Cell Imaging
Image Aquisition
Image Processing And Analysis
Real-time qPCR
Widefield Microscopy
1.) Jedariforoughi A, Burke R, Chesak A, Gonzalez Hernandez JL, Hanson RL (2025 Oct 21). JNK activation dynamics drive distinct gene expression patterns over time mediated by mRNA stability NPJ Systems Biology and Applications, 11(), 117. . PMCID: 12540708.
2.) Otchere S, Shrestha P, Parmar HN, Perry JF, Hofmeister BL, Steyn M, Kaushik RS, Hoppe AD, Thiex NW, Hanson RL, Lopez-Mosqueda J, Imre G (2025 Oct 6). ALIX mediates reversible gasdermin-D pore formation via the endosomal pathway to limit pyroptosis by active membrane repair Cell Death & Disease, 16(1), 681. . PMCID: 12501301.
3.) Katwal P, Aftab S, Nelson E, Hildreth M, Li S, Wang X (2025 Jul 25). Interferon-Induced Transmembrane Protein 3 (IFITM3) Restricts PRRSV Replication via Post-Entry Mechanisms Microorganisms, 13(8), 1737. . PMCID: 12388377.
4.) Steyn M, Lawson SR, Nelson E, Imre G (2025 Mar 21). Protocol for simultaneous detection of PRRS virus infection and dying cell populations by a flow cytometry double-staining approach. STAR protocols, 6(1), 103688. . PMID: 40073019.
5.) Sarao SK, Sandhu AK, Hanson RL, Govil T, Brözel VS (2025 Mar 20). Phenotypic heterogeneity in Bradyrhizobium diazoefficiens USDA 110 Research Square, (), rs.3.rs-5754266. . PMCID: 11957192.
6.) Koirala A, Alshibli NA, Das BK, Brözel VS (2025 Jan 6). Bacterial Isolation from Natural Grassland on Nitrogen-Free Agar Yields Many Strains Without Nitrogenase Microorganisms, 13(1), 96. . PMCID: 11768025.
7.) Sarao SK, Sandhu AK, Hanson RL, Govil T, Brözel VS (2025 Jan). Bradyrhizobium diazoefficiens cultures display phenotypic heterogeneity. ISME communications, 5(1), ycaf054. . PMID: 40235686.
8.) Wollman J, Wanniarachchi K, Pradhan B, Huang L, Kerkvliet JG, Hoppe AD, Thiex NW (2024 Nov 21). Mannose receptor (MRC1) mediates uptake of dextran by bone marrow–derived macrophages Molecular Biology of the Cell, 35(12), ar153. . PMCID: 11656472.
9.) Plesselova S, Calar K, Axemaker H, Sahly E, Bhagia A, Faragher JL, Fink DM, de la Puente P (2024 Oct). Multicompartmentalized Microvascularized Tumor-on-a-Chip to Study Tumor-Stroma Interactions and Drug Resistance in Ovarian Cancer. Cellular and molecular bioengineering, 17(5), 345-367. . PMID: 39513004.
10.) Wollman J, Wanniarachchi K, Pradhan B, Huang L, Kerkvliet JG, Hoppe AD, Thiex NW (2024 Aug 13). Mannose receptor (MRC1) mediates uptake of dextran in macrophages via receptor-mediated endocytosis. bioRxiv : the preprint server for biology, (), . . PMID: 39211167.
11.) Sandhu AK, Fischer BR, Subramanian S, Hoppe AD, Brözel VS (2024 Jun 2). Self-growth suppression in Bradyrhizobium diazoefficiens is caused by a diffusible antagonist bioRxiv, (), 2024.06.01.596975. . PMCID: 11160724.
12.) Sarao SK, Sandhu AK, Hanson RL, Govil T, Brözel VS (2024 Mar 28). Bradyrhizobium diazoefficiens cultures display phenotypic heterogeneity ISME Communications, 5(1), ycaf054. . PMCID: 11996625.
13.) Das K, Schulte M, Gerhart M, Bayoumi H, Paulson D, Fink DM, Parrish C, Willand-Charnley R (2024). Early in vitro results indicate that de-O-acetylated sialic acids increase Selectin binding in cancers. Frontiers in oncology, 14(), 1443303. . PMID: 39717751.
The mission of the FGCF is to provide access and training on a range of instruments in the areas of genomics, molecular biology, imaging, and chemical/metabolite analysis to support research on the SDSU campus.
