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SDSU-Functional Genomics Core Facility

South Dakota State University

Contact Info:

1390 College Ave

Rm 047

Brookings, SD 57007

https://www.sdstate.edu/biology-and-microbiology/functional-genomics-core-facility

Grants and Identifiers:

RRID: RRID:SCR_023786

Instrumentation:

Life Technologies QuantStudio 6 Real Time PCR System

QuantStudio 6 Flex system accommodates the interchange of a 96-well, 96-well Fast, or 384-well block. The system is delivered with one block type (a 96-well block in this case); other block types may be purchased separately. For a system that also supports TaqMan array cards, as well as automation, please see our QuantStudio 7 Flex system. The QuantStudio 6 Flex system can also be upgraded to a QuantStudio 7 Flex system at a later date, as your needs change. For the ultimate in productivity, consider the QuantStudio 12K Flex system, which also accommodates TaqMan OpenArray plates. [Product Link]

RRID:SCR_020239

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Nanodrop 2000c Spectrophotometer

Thermo Scientific NanoDrop 2000 and 2000c are full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, Protein and more. The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure sample volumes as small as 0.5?L. [Product Link]

RRID:SCR_020309

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Olympus IX70 Inverted Fluorescence Tissue Culture Microscope

Inverted fluorescence tissue culture microscope capable of imaging specimens in brightfield, darkfield, phase contrast, hoffman modulation contrast, fluorescence, and differential interference contrast modes. [Product Link]

RRID:SCR_018604

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Licor Odyssey Fc

Olympus Bx53 upright microscope

Olympus FV1200 confocal microscope

Services Provided:

Biolayer Interferometry (BLI)

CDNA Synthesis

Cell Imaging

Image Aquisition

Image Processing And Analysis

Real-time qPCR

Widefield Microscopy

Relevant Publications:

1.) Koirala A, Alshibli NA, Das BK, Brözel VS (2025 Jan 6). Bacterial Isolation from Natural Grassland on Nitrogen-Free Agar Yields Many Strains Without Nitrogenase Microorganisms, 13(1), 96. . PMCID: 11768025.


2.) Wollman J, Wanniarachchi K, Pradhan B, Huang L, Kerkvliet JG, Hoppe AD, Thiex NW (2024 Nov 21). Mannose receptor (MRC1) mediates uptake of dextran by bone marrow–derived macrophages Molecular Biology of the Cell, 35(12), ar153. . PMCID: 11656472.


3.) Wollman J, Wanniarachchi K, Pradhan B, Huang L, Kerkvliet JG, Hoppe AD, Thiex NW (2024 Aug 13). Mannose receptor (MRC1) mediates uptake of dextran in macrophages via receptor-mediated endocytosis. bioRxiv : the preprint server for biology, (), . . PMID: 39211167.


4.) Sandhu AK, Fischer BR, Subramanian S, Hoppe AD, Brözel VS (2024 Jun 2). Self-growth suppression in Bradyrhizobium diazoefficiens is caused by a diffusible antagonist bioRxiv, (), 2024.06.01.596975. . PMCID: 11160724.


Description:

The mission of the FGCF is to provide access and training on a range of instruments in the areas of genomics, molecular biology, imaging, and chemical/metabolite analysis to support research on the SDSU campus.