University of Freiburg
Contact Info:
Marie Follo
Zentrum für Translationale Zellforschung
Breisacher Strasse 115
Freiburg 79106
https://www.uniklinik-freiburg.de/medizin1/forschung/lighthouse-core-facility.html
Grants and Identifiers:
RRID: RRID:SCR_023785
Other Citation IdentifiersDFG Project Number 450392965
Medical Faculty, University of Freiburg Project Number 2025/A2-Fol
Medical Faculty, University of Freiburg Project Number 2025/B3-Fol
Instrumentation:
BD Biosciences FACSAria III Cell Sorter
Instrument for cell sorting by BD Biosciences where positive air pressure forces sample cell through an optically gel-coupled cuvette . Hydrodynamic focusing guides particles in a single-file stream through the cuvette, where laser light intercepts the stream at the sample interrogation point.Fluorescence activated cell sorting instrument. Flow cytometer as high speed fixed alignment benchtop cell sorter. Enables multicolor analysis of up to 13 fluorescent markers and two scatter parameters at time. [Product Link]
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BD FACSAria Fusion Cell Sorter
Cell sorter has UV, Violet, Blue, Yellow/Green and Red lasers and can detect up to 18 colors. Can sort up to 4 populations simultaneously. Samples can be sorted into 12 x 75 mm FACS tubes or 1.5 ml Eppendorf tubes. Uses BD FACSDiva software to control setup, acquisition, and analysis of flow cytometry data from operator workstation. [Product Link]
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(Equipment No Longer Active At this Facility)
BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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Beckman Coulter CytoFLEX LX Flow Cytometer
This model is equipped with four lasers (405, 488 561 and 638nm), and is capable of detecting FSC, SSC and 16 colors. [Product Link]
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Beckman Coulter CytoFLEX SRT Cell Sorter
Offers multiple configurations with the option to easily upgrade. Instrument can be configured to use up to four lasers and up to 15-color detection to identify subtle differences between cells. Core Facility’s CytoFLEX SRT is four-laser platform (405, 488, 561 and 638nm) instrument capable of detecting FSC, SSC and 15-colors and is able to sort up to four separate populations from the same sample, and deposit them in variety of tubes and plates. Operates under CytExpert SRT Software. [Product Link]
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Beckman Coulter Life Sciences MoFlo Astrios EQ Cell Sorter
High speed flow cytometry sorter. [Product Link]
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(Equipment No Longer Active At this Facility)
Olympus IX83 Automated Fluorescence Microscope
The Olympus IX83 inverted microscope has been integrated into our IXplore systems. IXplore Systems are designed to provide solutions-based packages that suit your research application needs. [Product Link]
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Roche Light Cycler 480 qPCR Real Time PCR System
Real time PCR device that allows use of additional thermal block cycler units 96 well and 384 well format. [Product Link]
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Sartorius IncuCyte S3 Live Cell Analysis System
System that automatically captures and analyzes images of living cells around-the-clock for days, weeks, or months, while cells remain undisturbed inside standard tissue culture incubator. Kinetic, image-based measurements. Used for cell monitoring and surveillance, cell health and viability, migration and invasion, phenotypic cell based assays. [Product Link]
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(Equipment No Longer Active At this Facility)
Sony Biotechnology Inc. SP6800 Spectral Cell Analyzer
The Sony SP6800 Spectral Analyzer is an analyzer that uses spectral technology to collect photons from 420nm to 800nm. Spectral technology also simplifies multicolor panel design, by eliminating bandpass filters and conventional compensation matrices while delivering data and simplifying visualization for the study of heterogeneous populations. Global standardization mode automatically sets the system to a master specification with a single click. This capability eliminates instrument variability from day to day and across multiple instruments for greater reliability. Advanced electronics and patented optical technologies bring simplicity to Spectral Analysis workflows. Sony’s patented Flowpoint™ core stability and tracking system and automated QC ensure the highest resolution possible of target populations. [Product Link]
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Sony ID7000 Spectral Cell Analyzer
Flow cytometer designed for comprehensive, high-parameter analysis of heterogeneous cell populations. It utilizes spectral technology supporting up to 7 lasers and 186 detectors to detect dim/rare populations and distinguish over 44 colors, making it ideal for advanced immunology and oncology research. [Product Link]
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(Equipment No Longer Active At this Facility)
Zeiss LSM 710 Confocal Inverted Microscope
LSM 710 is a confocal is an inverted microscope that enables confocal microscopy for a wide variety of applications. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. [Product Link]
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(Equipment No Longer Active At this Facility)
Zeiss LSM 880 with Airyscan Confocal Laser Scanning Microscope
Fully enclosed laser scanning confocal imaging microscopefor heating and environmental control with media perfusion capabilities and CO2 control. It can be used for Live Cell Imaging, FRET and Anisotropy, FRAP and FLIP, FCS, Photoactivation/ -conversion. [Product Link]
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(Equipment No Longer Active At this Facility)
ZEISS LSM 980 with Airyscan 2 Microscope
Upright laser scanning confocal microscope with Airyscan 2 technology. Airyscan module is new detector concept featuring 32 channel GaAsP-PMT area detector. Airyscan detector brings simultaneous improvements in spatial resolution and signal-to-noise ratio in comparison with conventional confocal microscopy. [Product Link]
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(Equipment No Longer Active At this Facility)
Zeiss LSM 980 NLO MP with Airyscan confocal microscope
(Equipment No Longer Active At this Facility)
Zeiss CellDicoverer 7 with LSM 900
Combined confocal/widefield system with LSM 900 scan head and climate chamber for live cell imaging.
Zeiss CellDiscoverer 7 with LSM 900 AiryScan2
Combined confocal/widefield system with LSM 900 scan head and AiryScan2. Fully integrated climate chamber for optimal live cell imaging.
(Equipment No Longer Active At this Facility)
Zeiss LSM 980 NLO MP with Airyscan Confocal
Confocal Microscope
Services Provided:
3D & 4D Image Processing Stations
Confocal/ Super Resolution Microscope
Fluorescence Microscope With Optical Sectioning Capability
High Dimensional Flow Cytometry Analysis
Relevant Publications:
Description:
Lighthouse Core Facility (LCF) is a recognized core facility of the Medical Faculty of the University of Freiburg. It is also registered in the RIsources Database of the German Research Foundation (DFG) under the number RI_00416. Lighthouse specializes in the areas of flow cytometry and cell sorting; light microscopy (confocal, widefield fluorescence, high content screening, lightsheet, slide scanning, highly multiplexed microscopy); and digital PCR / QPCR. It is located at the Medical Center - University of Freiburg. The Lighthouse host institutions include the Dept. of Medicine I, the Center for Chronic Immunodeficiency (CCI), the CCCF / DKTK, and the Medical Faculty of the University of Freiburg.
