University of California Berkeley
Contact Info:
Mary West
UC Berkeley, QB3
B129 Stanley Hall
Berkeley, CA 94720-3220
https://qb3.berkeley.edu/facility/ctaf-htsf/
Grants and Identifiers:
RRID: RRID:SCR_023741
Instrumentation:
Bio-Rad CFX96 Real-Time PCR Detection System
The CFX96 Touch System is a real-time PCR detection system that combines optical technology with temperature control. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, thermal cycler performance, unrivaled stand-alone functionality, and easy-to-use software, the CFX96 Touch System is designed to advance your qPCR. [Product Link]
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BioTek Lionheart FX Automated Microscope
Microscope that offers up to 60x air; 60x and 100x oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. [Product Link]
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Bruker Ultima 2Pplus Multiphoton Microscope
Multiphoton microscope that is designed for intravital imaging, with fully motorized control of objective X-Y-Z position, as well as two axes of rotation for precise imaging orientation. Second scan path enables simultaneous imaging and photoactivation. [Product Link]
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(Equipment No Longer Active At this Facility)
Thermo Fisher Attune Nxt Nxt
2020 Attune NxT Flow Cytometer makes multiparametric flow cytometry available to both new and experienced researchers. [Product Link]
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Services Provided:
Luminex Bead Based Cytokine Assays
Multiplexed Immunofluorescence
Processing & Embedding- Histology
Shared Instrumentation Oversight & Maintenance
Single Particle Extracellular Vesicle Flow Cytometry
Relevant Publications:
Description:
The QB3 Cell and Tissue Analysis Facility provides cell culture equipment and instrumentation needed to grow and assess mammalian cells including flow cytometry, hypoxia chambers, automated epifluorescence, confocal, and multiphoton microscopy, as well as a histology suite with cryosectioning equipment.
