University of British Columbia
Andy Sham
RM 211, W 28th Ave,
Vancouver, BC V5Z 4H4
https://www.bcchr.ca/gut4health
RRID: RRID:SCR_023673
Bio-Rad QX200 AutoDG Droplet Digital PCR System
Droplet generator that provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. [Product Link]
RRID:SCR_019714
Cell Culture
Clinical Research
Droplet Digital PCR
ELISA
Metagenomics Analysis
Microbiological Culturing
Microbiome
Mycoplasma Detection
Real-time qPCR
Tissue Culture
1.) Graef FA, Berger B, Bahr LS, Stange R, Michalsen A, Paul F, Vallance BA, Jacobson K (2025). Fasting elicits gut microbiome signature changes that extend to type 1 diabetes patients. Frontiers in endocrinology, 16(), 1623800. . PMID: 40881123.
2.) Ghezzi H, Fan YM, Ng KM, Burckhardt JC, Pepin DM, Lin X, Ziels RM, Tropini C (2024 Jul 9). PUPpy: a primer design pipeline for substrain-level microbial detection and absolute quantification mSphere, 9(7), e00360-24. . PMCID: 11288016.
3.) Lunken GR, Golding L, Schick A, Majdoubi A, Lavoie PM, Vallance BA (2022 Dec 22). Gut microbiome and dietary fibre intake strongly associate with IgG function and maturation following SARS-CoV-2 mRNA vaccination. Gut, (), . pii: gutjnl-2022-328556. doi: 10.1136/gutjnl-2022-328556. ID: 36549875.
4.) Graef FA, Celiberto LS, Allaire JM, Kuan MTY, Bosman ES, Crowley SM, Yang H, Chan JH, Stahl M, Yu H, Quin C, Gibson DL, Verdu EF, Jacobson K, Vallance BA (2021 Aug). Fasting increases microbiome-based colonization resistance and reduces host inflammatory responses during an enteric bacterial infection. PLoS pathogens, 17(8), e1009719. . ID: 34352037.
5.) Lunken GR, Tsai K, Schick A, Lisko DJ, Cook L, Vallance BA, Jacobson K (2021). Prebiotic Enriched Exclusive Enteral Nutrition Suppresses Colitis via Gut Microbiome Modulation and Expansion of Anti-inflammatory T Cells in a Mouse Model of Colitis. Cellular and molecular gastroenterology and hepatology, 12(4), 1251-1266. . ID: 34214707.
The primary goal of Gut4Health is to facilitate microbiome studies for researchers at BC Children’s Hospital Research Institute and the University of British Columbia. The facility is a centralized and cost-effective resource for processing and storing biological specimens, and sequencing and analysis of microbial communities for various research studies, including basic science, clinical, translational, and epidemiological projects.
