University of Pennsylvania
Contact Info:
Drew Weissman
, PAhttps://rnainnovation.med.upenn.edu/scientific-groups/mrna-core/
Grants and Identifiers:
RRID: RRID:SCR_023665
Instrumentation:
Services Provided:
Relevant Publications:
Description:
The Engineered mRNA and Targeted Nanomedicine Core is designed to address infrastructural barrier to clinical and translational research in gene therapy, vaccine development (cancer and infectious disease), stem cell reprograming, and other non-viral gene therapy-based applications by providing high quality in vitro transcribed messenger RNA (IVT-mRNA) and lipid nanoparticles (LNPs). Our goal and focus are to lower the experimental barriers to PIs interested in exploring the mRNA technology, and lipid nanoparticles for various applications including tissue and cell targeting. We offer the following services: (1) Optimization and production of in vitro transcribed mRNA. (2) Small-scale production of sequence optimized in vitro transcribed mRNA. (3) Incorporation of modified nucleosides to modulate activity. (4) Off the shelf in vitro transcribed mRNA encoding reporter genes (i.e. eGFP, mCherry, and Luc), or cancer antigens (i.e. OVA). (5) Labelled mRNA (i.e. Cy 3, Cy 5.5, Cy 7) for nanoparticle tracking and microscopy. (6) Encapsulation into commercially available proprietary (i.e. MC3, KC2 …) and non-proprietary lipid nanoparticles (i.e. DOTAP…) through the LNP core.
