Université Catholique de Louvain
Davide Brusa
Avenue Hippocrate, 55
Brussels 1000
https://uclouvain.be/en/research-institutes/irec/cytoflux
RRID: RRID:SCR_023664
Agilent NovoCyte Advanteon Flow Cytometer
Advanteon Flow Cytometer accommodates multicolor flow cytometry assays and provides flexibility of 1, 2, or 3 laser options. [Product Link]
RRID:SCR_019522
BD Scientific FACSCanto™ Flow Cytometer
The BD FACSCanto™ is a flow cytometry system that performs reliable performance, accuracy, and ease-of-use for clinical diagnostic services. The BD FACSCanto system has 10-color capability, optical enhancements, and a 4-3-3 configuration deliver high sensitivity and resolution for accurate results. [Product Link]
RRID:SCR_018055
Miltenyi AutoMACS Magnetic Cell Sorter
Benchtop manager cell sorter for cell isolation in automated workflows. It sorts cells directly from whole blood or bone marrow without density gradient centrifugation or erythrocyte lysis. [Product Link]
RRID:SCR_018596
Miltenyi gentleMACS Dissociator Tissue Dissociator
Benchtop instrument for semi automated dissociation of tissues into single cell suspensions or thorough homogenates. Single sample or two samples in parallel can be processed. [Product Link]
RRID:SCR_020267
Analysis Workstations
Assays and Measurements
Blood Cell Count
Cell Sorting
Clinical Research
Cloning
Consultation
Cytokine Assays
Data Analysis
Data Analysis And Interpretation
Data Processing
Data Processing And Interpretation
Data Visualization
DNA Analysis
FACS Cell Sorting
Flow Cytometric Analysis
Flow Cytometry Data Analysis
High Dimensional Flow Cytometry Analysis
Immunofluorescence
Multi-color Flow Cytometry
Phenotyping
Plate Reader
Single Particle Extracellular Vesicle Flow Cytometry
Single-Cell Analysis
Staining
Training
1.) Dadashzadeh A, Moghassemi S, Grubliauskaité M, Vlieghe H, Brusa D, Amorim CA (2022 Dec 26). Medium supplementation can influence the human ovarian cells in vitro. Journal of ovarian research, 15(1), 137. . ID: 36572931.
2.) van Dievoet MA, Brusa D, Octave M, Horman S, Stephenne X (2022 Oct 3). Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel. Platelets, 33(7), 1096-1099. doi: 10.1080/09537104.2022.2027359. ID: 35037555.
3.) Capeloa T, Krzystyniak J, d (2022 Mar 16). MitoQ Inhibits Human Breast Cancer Cell Migration, Invasion and Clonogenicity. Cancers, 14(6), . . ID: 35326667.
4.) d (2021 Sep 26). The Short-Term Exposure to SDHI Fungicides Boscalid and Bixafen Induces a Mitochondrial Dysfunction in Selective Human Cell Lines. Molecules (Basel, Switzerland), 26(19), . . ID: 34641386.
5.) Piaggeschi G, Rolla S, Rossi N, Brusa D, Naccarati A, Couvreur S, Spector TD, Roederer M, Mangino M, Cordero F, Falchi M, Visconti A (2021). Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women. Frontiers in immunology, 12(), 637974. . ID: 33767708.
6.) Orsi M, Palmai-Pallag M, Yakoub Y, Ibouraadaten S, De Beukelaer M, Bouzin C, Bearzatto B, Ambroise J, Gala JL, Brusa D, Lison D, Huaux F (2021). Monocytic Ontogeny of Regenerated Macrophages Characterizes the Mesotheliomagenic Responses to Carbon Nanotubes. Frontiers in immunology, 12(), 666107. . ID: 34194430.
7.) Chae H, Augustin R, Gatineau E, Mayoux E, Bensellam M, Antoine N, Khattab F, Lai BK, Brusa D, Stierstorfer B, Klein H, Singh B, Ruiz L, Pieper M, Mark M, Herrera PL, Gribble FM, Reimann F, Wojtusciszyn A, Broca C, Rita N, Piemonti L, Gilon P (2020 Dec). SGLT2 is not expressed in pancreatic α- and β-cells, and its inhibition does not directly affect glucagon and insulin secretion in rodents and humans. Molecular metabolism, 42(), 101071. . ID: 32896668.
8.) Schoonjans CA, Mathieu B, Joudiou N, Zampieri LX, Brusa D, Sonveaux P, Feron O, Gallez B (2020 Oct 15). Targeting Endothelial Cell Metabolism by Inhibition of Pyruvate Dehydrogenase Kinase and Glutaminase-1. Journal of clinical medicine, 9(10), . . ID: 33076309.
9.) Montiel V, Bella R, Michel LYM, Esfahani H, De Mulder D, Robinson EL, Deglasse JP, Tiburcy M, Chow PH, Jonas JC, Gilon P, Steinhorn B, Michel T, Beauloye C, Bertrand L, Farah C, Dei Zotti F, Debaix H, Bouzin C, Brusa D, Horman S, Vanoverschelde JL, Bergmann O, Gilis D, Rooman M, Ghigo A, Geninatti-Crich S, Yool A, Zimmermann WH, Roderick HL, Devuyst O, Balligand JL (2020 Oct 7). Inhibition of aquaporin-1 prevents myocardial remodeling by blocking the transmembrane transport of hydrogen peroxide. Science translational medicine, 12(564), . pii: eaay2176. doi: 10.1126/scitranslmed.aay2176. ID: 33028705.
10.) Zampieri LX, Grasso D, Bouzin C, Brusa D, Rossignol R, Sonveaux P (2020 Sep). Mitochondria Participate in Chemoresistance to Cisplatin in Human Ovarian Cancer Cells. Molecular cancer research : MCR, 18(9), 1379-1391. doi: 10.1158/1541-7786.MCR-19-1145. ID: 32471883.
11.) Alevra Sarika N, Payen VL, Fléron M, Ravau J, Brusa D, Najimi M, Pauw E, Eppe G, Mazzucchelli G, Sokal EM, Rieux AD, Taghdouini AE (2020 May 30). Human Liver-Derived Extracellular Matrix for the Culture of Distinct Human Primary Liver Cells. Cells, 9(6), . . ID: 32486126.
12.) Verhaegen C, Kautbally S, Zapareto DC, Brusa D, Courtoy G, Aydin S, Bouzin C, Oury C, Bertrand L, Jacques PJ, Beauloye C, Horman S, Kefer J (2020). Early thrombogenicity of coronary stents: comparison of bioresorbable polymer sirolimus-eluting and bare metal stents in an aortic rat model. American journal of cardiovascular disease, 10(2), 72-83. . ID: 32685265.
CytoFlux consists of tools for tissues dissociation and single cell preparation, analysis and cell sorting under the supervision of a research logistician.
It offers the following services:
- Samples preparations and multiparametric panels consultancy
- Acquisition and analysis of multiparametric samples
- Cell Sorting
- Data Analysis
