University of Florida Health
George Tsaprailis
130 Scripps Way
1B2
Jupiter, FL 33458
https://scripps.ufl.edu/cores-and-technologies/mass-spectrometry-and-proteomics/
RRID: RRID:SCR_023576
Thermo Scientific Orbitrap Fusion Tribrid Mass Spectrometer
Combines quadrupole, ion trap, and Orbitrap mass analysis into Tribrid architecture to provide depth of analysis. Enables analyzing of low-abundance, high-complexity samples to identify compounds and to quantify accurately and elucidate structures thoroughly. [Product Link]
RRID:SCR_020573
Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer
Identify, quantify and confirm more compounds rapidly and with confidence using the Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer. This benchtop LC-MS/MS system combines quadruple precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection to deliver performance and versatility. The Q Exactive Mass Spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology. [Product Link]
RRID:SCR_020565
Bruker Daltonics Tims-TOF Pro2
Mass Spectrometry
Protein Extraction/Purification
Proteomics
Quantitative Phosphoproteomics
Quantitative Proteomics - TMT
1.) Watkins JM, Douglas CJ, Cusic R, Seath CP, Burke JM (2026 Mar 24). RNase L regulates the antiviral proteome by accelerating mRNA decay, inhibiting nuclear mRNA export, and repressing transcription. Cell reports, 45(3), 116924. . PMCID: 13206644.
2.) Dagar S, Fernandez A, Ramírez-Jarquín UN, Lopez-Huerta VG, Mirza E, Mohapatra C, Zuniga I, Urban NT, Crynen G, Tsaprailis G, Subramaniam S (2026 Mar 20). Membrane-associated Rhes-Slc4a7 complex orchestrates tunneling nanotube formation and mutant Huntingtin spread. Science Advances, 12(12), . . PMCID: 13004034.
3.) Wang S, Brischigliaro M, Zhang Y, Wu C, Zheng W, Barrientos A, Xiong Y (2026 Feb 9). Structural basis of TACO1-mediated efficient mitochondrial translation. Nature communications, 17(1), . . PMCID: 12996406.
4.) Wang Y, Zhao C, Wang T, Steele A, Joshi M, Mori S, Chatterjee A, Shen B, Disney MD, Wasa M (2025 Nov 26). Chemical Tagging of N-Alkylamine-Containing Natural Products and Pharmaceuticals through C(sp(3))-H Functionalization. Journal of the American Chemical Society, 147(47), 43424-43437. . PMCID: 12908569.
5.) Gomes FP, Durbin KR, Schauer K, Nwachukwu JC, R Kobylski R, Njeri JW, Seath CP, Saviola AJ, McClatchy DB, Diedrich JK, Garrett PT, Papa AB, Ciolacu I, Kelleher NL, Nettles KW, Yates JR 3rd (2025 Aug). Native top-down proteomics enables discovery in endocrine-resistant breast cancer. Nature chemical biology, 21(8), 1205-1213. . PMCID: 12307125.
6.) Bertone AL, Reinemeyer C, Tsaprailis G, Ragland D, Leise B (2025 Jul 23). Cryopreserved equine umbilical cord tissue allograft characterization and biocompatibility in vivo in musculoskeletal tissues: a controlled study BMC Medicine, 23(), 439. . PMCID: 12288288.
7.) Watkins JM, Douglas CJ, Cusic R, Seath CP, Burke JM (2025 Jul 21). RNase L regulates the antiviral proteome by accelerating mRNA decay, inhibiting nuclear mRNA export, and repressing RNAPII-mediated transcription. bioRxiv : the preprint server for biology, (), . . PMID: 40777276.
8.) Chanda K, Grinman E, Clark K, Sadhu A, Raveendra B, Swarnkar S, Puthanveettil SV (2025 Jun 24). The lncRNA Gas5 is an activity-responsive scaffold for mediating cAMP-dependent synaptic plasticity Science signaling, 18(892), eadn2044. . PMCID: 12285921.
9.) Chanda K, Grinman E, Clark K, Sadhu A, Raveendra B, Swarnkar S, Puthanveettil SV (2025 Jun 24). The lncRNA Gas5 is an activity-responsive scaffold that mediates cAMP-dependent synaptic plasticity. Science signaling, 18(892), eadn2044. . PMID: 40554602.
10.) Tong J, Watkins JM, Burke JM, Kodadek T (2025 Jun 14). Assessing the Suitability of Deubiquitylases As Substrates For Targeted Protein Degradation bioRxiv, (), 2025.06.13.659525. . PMCID: 12259181.
11.) Brischigliaro M, Krüger A, Moran JC, Antonicka H, Ahn A, Shoubridge EA, Rorbach J, Barrientos A (2024 Sep 9). The human mitochondrial translation factor TACO1 alleviates mitoribosome stalling at polyproline stretches. Nucleic acids research, 52(16), 9710-9726. . PMID: 39036954.
12.) Carter TR, Milosevich N, Dada L, Shaum JB, Sharpless KB, Kitamura S, Erb MA (2024 Apr 4). SuFEx-based chemical diversification for the systematic discovery of CRBN molecular glues Bioorganic & medicinal chemistry, 104(), 117699. . PMCID: 11195152.
13.) Dagar S, Sharma M, Tsaprailis G, Tapia CS, Crynen G, Joshi PS, Shahani N, Subramaniam S (2024 Apr). Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease. Molecular & cellular proteomics : MCP, 23(4), 100746. . PMID: 38447791.
The Mass Spectrometry and Proteomics Core has one mission: to provide a wide range of state-of-the-art mass spectrometry-based proteomics services to assist with protein characterization, identification and quantification, and apply these technologies to solve relevant biological problems.
