Search |

Add/Edit My Facility

Mass Spectrometry and Proteomics

University of Florida Health

Contact Info:

George Tsaprailis

130 Scripps Way

1B2

Jupiter, FL 33458

https://scripps.ufl.edu/cores-and-technologies/mass-spectrometry-and-proteomics/

Grants and Identifiers:

RRID: RRID:SCR_023576

Instrumentation:

Thermo Scientific Orbitrap Fusion Tribrid Mass Spectrometer

Combines quadrupole, ion trap, and Orbitrap mass analysis into Tribrid architecture to provide depth of analysis. Enables analyzing of low-abundance, high-complexity samples to identify compounds and to quantify accurately and elucidate structures thoroughly. [Product Link]

RRID:SCR_020573

cite this instrument

Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer

Identify, quantify and confirm more compounds rapidly and with confidence using the Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer. This benchtop LC-MS/MS system combines quadruple precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection to deliver performance and versatility. The Q Exactive Mass Spectrometer is equally useful for untargeted or targeted screening and a broad range of qualitative and quantitative applications in drug discovery, proteomics, environmental and food safety, clinical research and forensic toxicology. [Product Link]

RRID:SCR_020565

cite this instrument

Bruker Daltonics Tims-TOF Pro2

Services Provided:

Mass Spectrometry

Protein Extraction/Purification

Proteomics

Quantitative Phosphoproteomics

Quantitative Proteomics - TMT

Relevant Publications:

1.) Brischigliaro M, Krüger A, Moran JC, Antonicka H, Ahn A, Shoubridge EA, Rorbach J, Barrientos A (2024 Sep 9). The human mitochondrial translation factor TACO1 alleviates mitoribosome stalling at polyproline stretches. Nucleic acids research, 52(16), 9710-9726. . PMID: 39036954.


2.) Carter TR, Milosevich N, Dada L, Shaum JB, Sharpless KB, Kitamura S, Erb MA (2024 Apr 4). SuFEx-based chemical diversification for the systematic discovery of CRBN molecular glues Bioorganic & medicinal chemistry, 104(), 117699. . PMCID: 11195152.


3.) Dagar S, Sharma M, Tsaprailis G, Tapia CS, Crynen G, Joshi PS, Shahani N, Subramaniam S (2024 Apr). Ribosome Profiling and Mass Spectrometry Reveal Widespread Mitochondrial Translation Defects in a Striatal Cell Model of Huntington Disease. Molecular & cellular proteomics : MCP, 23(4), 100746. . PMID: 38447791.


Description:

The Mass Spectrometry and Proteomics Core has one mission: to provide a wide range of state-of-the-art mass spectrometry-based proteomics services to assist with protein characterization, identification and quantification, and apply these technologies to solve relevant biological problems.