Biotage Selekt Automated Flash Purification System

The Biotage ® Selekt is an automated flash purification system. The system has two column channels with an external ELSD (evaporative light-scattering detector) for flash chromatography to purify compounds with little or no UV absorption. Features: Column Channels: 2 Flow Rate Range: 1-300 mL/min Pressure Range: 0-30 bar (0-3,000 kPa: 0-435 psi) QR code reader RFID enabled rack

Biotage V-10 Touch Evaporator

The Biotage® V-10 Touch rapidly dries samples dissolved in both aqueous and organic solvents. It easily evaporates HPLC fractions from purification, high boiling point solvents from synthesis and features a dry-down onto silica technique for easier dry load capabilities prior to purification. Solvent Compatibility: Boiling points from 30 °C to 160 °C (up to 205 °C with an external vacuum pump). The system is not suitable for solutions containing HCl at any concentration. Features: Heating: 20 ºC to 70 ºC Rotational Speed: 3000 to 8000 rpm Condenser: Refrigerated condenser temperature -25 ºC. Automated and manual draining; and manual defrost Vial Compatibility: 30 and 20 mL scintillation vials and 8 and 4 mL vials Solvent Reclamation: Up to 98% of solvent vapors under typical conditions Safety Concerns: The Biotage V-10 is not compatible with the following solutions: HCl (hydrochloric acid) NaOH (sodium hydroxide) HNO₃ (nitric acid) H₂SO₄ (sulphuric acid) H₂O₂ (hydrogen peroxide) NaClO (sodium hypochlorite)

Carbon M2 3D Printer

The Carbon M2 3D printer uses continuous liquid interface production technology (CLIP) to rapidly create physical objects from 3D models. Carbon's tunable photochemical process involves controlling the oxygen flux through a light- and oxygen-permeable window, which creates a thin (10’s of micrometers thick) region within the resin pool where oxygen inhibits photopolymerization. As cross-sectional images of the 3D model are projected into the resin pool from below, the physical object continuously materializes from just above this thin oxygen-rich region. This technology produces “layerless” components with smooth external surfaces and solid cross-sectional areas of printed features. Components created with the Carbon M2 are printed roughly 25 to 100 times faster than traditional SLA printing and have more consistent mechanical properties. Specifications: Build Volume (X x Y x Z): 189 x 118 x 326 mm (7.4 x 4.6 x 12.8 in) XY; Z Resolution: 75 µm; 25, 50, or 100 µm General Accuracy: +/- 70 µm + 1 µm per mm Production Repeatability Accuracy: +/- 40 µm

Cellink BioX 3D Bioprinter

Applications Drug delivery, Soft robotics, 3D Cell Culture, Biomaterial Development, Tissue Models & Tissue Engineering, Organs-in-a-chip. Print living cells. Supports different resin system with different gelation stimuli. Advanced Functionality and Versatility Compatible with a wide range of materials. Precise printhead and printbed temperature control: printhead temperature range of 4°C to 250°C and a printbed temperature range between 4°C and 65°C; compatible with temperature-sensitive materials like collagen and gelatin. 1 µm motor resolution Universal Compatibility The BIO X’s open material platform is an open system that is compatible with a wide range of materials. Compatible with custom made and commercial inks. 7 exchangeable print heads. Patented Clean Chamber Technology Built-in UV sterilization and a HEPA filter bring the sterility of the biosafety cabinet to the benchtop. Dual high-powered fans channel air through a HEPA H14 filter to remove 99.995% of unwanted particles and microorganisms as part of the Clean Chamber™ technology, which also includes UV-C germicidal lights, rounded edges and positive air pressure. Initiate Clean Chamber before beginning your experiment to ensure full sterility around the print area for the duration of the print.

Cellink Lumen X DLP Printer

The Lumen X leverages digital light processing (DLP) printing to offer users high resolution, high throughput and high fidelity, enhancing applications in microfluidics, cell-laden hydrogels, macroporous structures and more. The BioPACIFIC MIP Lumen X bioprints with with near UV light (385 nm) and can achieve complex branching and tapering of vessels. Advanced Functionality and Versatility Compatible with a wide range of biocompatible photoinks. Compared to extruded scaffolds, Lumen X scaffolds are significantly more isotropic, allowing users to control mechanical properties with geometry in all dimensions. DLP technology coupled with Volumetric PhotoInks allow the Lumen X to build watertight channels in hydrogels with greater consistency and precision than extrusion. Printing Specifications Projected Image: 1280 x 800 pixels Pixel resolution (XY): 50 µm Z-precision (motor-driven): 5µm Max build volume 65 x 35 x 50 mm Projected light wavelength: 385 nm Heated platform: up to 60°C Disposable, quick-change sterile vats Advantages Print living cells Disposable, quick change sterile vats Heated platform

Chemspeed Robotic Polymer Synthesis Platform

The Robotic Polymer Synthesis Platform integrates robotic handling with an automated powder and liquid handler/dispenser and high-throughput characterization, enabling the characterization of monomers, polymers, and material properties on the same timescale with which they can be made, overcoming traditional challenges to high-throughput materials discovery. Additional features include temperature-controlled mixing stations with small scale reaction plates, photochemistry capability, and the option to conduct reactions under superheated conditions and pressure.

Custom-built High-Throughput Micro-Rheometer

The custom-built microrheology platform enables the rapid analysis of material viscoelasticity and provides insights into microstructure, emergence of phase transitions, material stability, and the presence of intermolecular interactions. The system includes motorized stages for high-speed scanning through multi-well samples, and imaging- and scattering-based tools for detection of material fluctuations or deformations under loading. User-friendly software facilitates rapid post-processing and data analysis. The microrheology platform is fully integrated onto an inverted optical microscope system (Zeiss Axio Observer) capable of fluorescence (DAPI, FITC, TRITC, DSRed, CY5, and 96 HE), Phase Contrast, Bright-field, and Dark Field imaging techniques. The imaging platform includes six different objectives paired with a CMOS camera capable of 128 fps at full resolution and up to 1000 fps when cropped. Long-term observations of living biological samples are permitted by customized environmental controls (temperature, RH, and CO2).

Custom-built Next Generation SAXS/WAXS System

A state-of-the-art liquid gallium X-ray source and a large-area pixel array detector are the key components of a fully dedicated small and wide-angle X-ray scattering (SAXS/WAXS) system. The high brilliance liquid metal jet X-ray source is a newly developed X-ray microfocus source that can increase the X-ray flux by 10X, which is crucial for measurements on weakly scattering biological and polymeric samples. The pixel array detector has single photon counting sensitivity and a large active area (5X larger) which dramatically increases the speed of data collection. The combination of the high flux source and large area detector enables data collection with a 50X increase in speed or sensitivity, thus providing a significant boost in research capability for a diverse array of projects.

Custom-built Solution Mask Liquid Lithography (SMaLL) 3D printing system

A custom-built Solution Mask Liquid Lithography (SMaLL) 3D printing system enables the direct incorporation of new, novel bioderived monomers. SMaLL provides users with a universal 3D printing strategy based on orthogonal material sets, enabling users to precisely tune properties and applications of 3D printed objects with properties ranging from rigid to flexible, stimuli-responsive to cross-linked thermosets and biocompatible in the same object. This 3D printing system consists of a scanner, multiple-wavelength projection source, an automated build stage, and multiple reservoirs for user-defined resins, and is meant to provide users with a versatile and flexible tool that can use in-house dual-resin formulations or user-designed materials.

Formlabs Form2 SLA Printer

The Formlabs Form 2 printers are liquid resin stereolithographic 3D printers capable of producing high resolution accurate models out of a variety of materials. Liquid resin printers use a bath of reactive resin which is precisely cured using a 405nm laser. This printer is particularly well suited for thin high aspect ratio features and models requiring great surface accuracy. The lab stocks several different solid and elastomeric resins. The build volume is 145 x 145 x 175 mm. The layer thickness is 25-100um. The FormWash station is used to clean residual resin, and the FormCure UV station is available for parts that benefit from post curing in a heated chamber with UV illumination.

Gyros Protein Technologies Prelude X Peptide Synthesizer

The Prelude X system (Gyros Protein Technologies) enables users to synthesize novel peptides and related structures (peptoids and other solid-phase synthesized sequence specific materials) not available for commercial purchase, providing a lower cost solution for producing peptides with non-natural amino-acids, and supporting the synthesis of branching or cyclic peptides. Multi-user accessibility is facilitated via 6 reaction stations, enabling different sequences, scales, and protocols on each reactor. Typical scales span 10 mg to 2 g of resin in 10- or 40-mL reaction vessels (RVs) with induction heating up to 90°C for each RV to accelerate difficult deprotections and couplings. Agitation is performed by nitrogen bubbling, oscillation mixing, or a combination of these. The Prelude X has also been pre-configured with a number of optimized methods for rapid peptide and peptoid synthesis.

Gyros Protein Technologies Symphony X Peptide Synthesizer

The Symphony X (Gyros Protein Technologies) is a flexible, automated synthesizer that enables the preparation of novel peptides, peptoids, and other solid-phase synthesis sequence specific materials. It provides a viable route to non-commercial products, branched or cyclic materials, and a low-cost solution for non-natural amino acid-based peptides. The system also includes the ability to perform in-situ pre-activation, special reagent additions with no waste of precious starting materials, as well as automated and fully customizable cleavage. Multi-user accessibility is facilitated via 24 fully independent reaction stations, enabling different sequences, scales, and protocols on multiple reactors simultaneously. Typical scales span 10 mg to 2 g of resin in 10- or 40-mL reaction vessels (RVs) with IR heating up to 90ºC for one RV to accelerate difficult deprotections and couplings. Agitation of each RV is performed by nitrogen bubbling, oscillation mixing, or a combination of these. The Symphony X at BioPACIFIC has also been pre-configured with a number of optimized methods for rapid peptide and peptoid synthesis.

MonoPrinter Mono1 DLP Printer

Mono1 is a light-driven printer that uses a 405 nm light to convert liquid resin into solid objects. Mono1 uses a projected light source (projector) to cure the entire layer at once. Specifications: Print technology: DLP Projected image: 1280 x 800 px Pixel resolution (XY): 25 – 25 µm Excitation wavelength: 405 nm Print speed: 25.4 mm/hour (varies with resin and layer thickness) Z-precision (motor-driven): 25 µm Max build volume: 96 X 56 X 130 mm (700 mL) Slicer software: MonoWare

MonoPrinter Mono3 DLP Printer

Mono3 is a light-driven printer that uses three different LEDs 459, 520, 617 nm to convert liquid resin into solid objects. Mono3 uses a Digital Light Processing (DLP) chip to project a patterned light that selectively exposes and hardens the resin. This technology allows for the entire layer to be exposed at once, allowing for fast build speeds independent of object complexity. Specifications: Print technology: DLP Projected image: 1280 x 800 px Pixel resolution (XY): 25 – 70 µm Layer thickness: 5 – 100 µm Excitation wavelength: 459, 520, 617 nm Power: 10 – 30 mW/cm^2 Print speed: 25.4 mm/hour (varies with resin and layer thickness) Z-precision (motor-driven): 25 µm Max build volume: 134 x 76 x 125 mm (1.27 L) Slicer software: MonoWare

MonoPrinter Mono3Z2 Panchromatic Printer

The Mono3Z2 panchromatic printer is a visible light printer that uses five different LED sources (405 nm (violet), 460 nm (blue), 525 nm (green), 615 nm (red), and 730 nm (NIR)) to print objects from 3D models. This printer can use two different LEDs at the same time to create multilateral objects using panchromatic photopolymer resins. This range of wavelengths can improve biocompatibility, enables a greater penetration depth, and reduces scattering. Specifications: Build area (X x Y): 38.4 x 21.6 mm Build volume (mL): 125 mL XY: Resolution: 20 µm Layer thickness: 10 - 300 µm Print Speed: 30 mm/hour maximum

Optics11 Pavone Multi-indenter

The Optics11 Pavone is a high-throughput mechanical characterization tool (modulus, adhesion, creep, stress relaxation). The instrument allows for testing of up to two 96-well plates in a single run, with Brightfield and Phase imaging capabilities. Calibrated Probes: Load: 200 pN - 2 mN Diameter: 3 - 50 µm Indentation speed: 0.01 - 100 µm/s Elastic modulus range: 1 kPa - 1 GPa Temperature: room temperature to 50 ºC Imaging capabilities: Brightfield Phase 20 x objective Mechanical Characterization modes: Quasi-static indentation Step-response for creep and stress relaxation Dynamic/oscillatory (0.01 - 10 Hz) Adhesion

Shimadzu Nexera Hybrid HPLC

The Shimadzu Nexera Hybrid HPLC system at BioPACIFIC MIP combines analytical chromatography for method development and fraction re-analysis with a preparative HPLC for fraction collection, eliminating the time-consuming tasks involved in moving samples and fractions between normally separate systems. The HPLC system at BioPACIFIC MIP further improves upon the typically UV-based fractionation processes and subsequent off-line mass spectrometry (MS) by inclusion of an inline single quadrupole MS detector. Direct on-line MS information ensures only fractions containing the target compound are collected. Efficiency in scale-up is maximized through use of a single system that can directly identify target compounds, automatically method scout for optimal column and focused gradients, and determine the purity of collected fractions following a purification run. The Shimadzu Nexera features: Dual prep and analytical flow paths with dedicated UV-Vis and PDA detector; A single quad MS detector with ESI and APCI ionization (2000 m/z max); Automated injection and column switching with matched C4 and C18 analytical and semi-prep columns; Verification of fraction purity with re-injection on the analytical side.

Stratasys F270 FDM Printer

The Stratasys F270 is a fast and precise fused deposition modeling (FDM) printer. The printer is typically set up with ABS and a soluble filament that is dissolved in a heated caustic bath. The build volume is 308 x 254 x 308 mm. The minimum layer thickness is 187um.

Texture Technologies Texture Analyzer

The Texture Technologies Texture Analyzer (TA) provides mechanical testing capabilities for soft materials, including small-strain contact mechanics (E, τporo, adhesive Gc), uniaxial compression (E, W, σf, τ, hysteresis), uniaxial extension (E, W, τ, σmax, εf, hysteresis), pure shear tests (E, W, Gc, fatigue, hysteresis), peel tests (Gc, crack velocity), lap shear tests (adhesive strength), puncture tests (Γo, Pc, k'E), and beam bending (E, σf). The TA can resolve ~1mN of force via a 50N load cell, and can accommodate scanning speeds of 0.01-40mm/sec over a distance of up to 370mm and with a 1µm displacement resolution. The TA is very light (33 pounds), so it can be used in a fume hood if there are any solvents that need to be vented.

ThermoFisher Laboratory Automation System - Living Biofoundry

An automated, high-throughput platform for gene assembly, amplification, transformation, strain growth, and metabolite analysis enables the production of bio-based monomers and polymers with precise repeat units, domains and chirality directly from microorganisms. By providing an equipment set focused on automation, control, and high-throughput pathway assembly at the gene level and metabolite detection at the cellular level, the Living Bioreactor will enable biosynthetic manufacturing of commodity monomers and polymers. A ThermoFisher Laboratory Automation System (LAS) serves as the cornerstone technology for the facility, enabling execution of automated customized synthetic biology and workflows at >500 samples-per-week. The LAS is equipped with over of over 10 functional instrumental components, including: a state-of-the-art SpinnakerTM microplate robot, automated incubators, reagent dispensers, thermal cyclers, plate sealer, and carousels/racks that are seamlessly integrated through the MOMENTUMTM application programming interface that is fully-compatible with laboratory information management systems (LIMS).

ThermoFisher Spectra 300 TEM - MicroED

MicroED excels at detecting atomic periodicities smaller than 1 Å and ab initio determination of molecular structures at this scale. Better than ~0.8 Å periodicities can be determined in crystals too small to be analyzed using traditional x-ray techniques. MicroED structure solutions reveal all atoms in an assembly, even hydrogens and for semi-crystalline biopolymers, and regions of local order/disorder are revealed. Importantly, in the best of cases, these structures can be rapidly determined (within 1h) from powders. Thus, the promising materials generated from the foundry (both small molecules and polymers) can rapidly be characterized by this novel electron diffraction technique. The Spectra 300C is a next-generation high-stability transmission electron microscope. The Spectra’s high brightness X-CFEG field emission gun offers high current in a focused electron probe for 4DSTEM acquisition. This tool is equipped with the latest software and hardware to enable rapid microED data collection at either room temperature or liquid nitrogen temperature. Key features: 30, 60, 120 to 300 kV Alignments C-TWIN Lens, Gap = 11 mm, Cs = 2.7 mm X-CFEG Cold Field Emission Gun Piezo and Motor Driven Computer-controlled Compustage +/- 80o single tilt Gatan Elsa Cryo-Transfer holder +/- 60/20o dual tilt analytical holder +/- 60/20o dual tilt Gatan 915 Cryo-Transfer holder STEM, Panther Detector, HAADF, iDPC Ceta-D High Speed (4k x 4k, 40 fps) 4DSTEM package MicroED Package (small beam blocker) S/TEM tomography package Velox, image analysis software (TFS) 5th Generation Octagon with fast kV changes

Ultimaker 3 Extended

The Ultimaker printers are Fused Deposition Modeling (FDM) printers with dual print heads and heated build platforms. They can accommodate most thermoplastic structural resins including support material. The build volume of the Ultimaker 3 is 215 x 215 x 300 mm, and 330 x 240 x 300 mm for the Ultimaker 5. The minimum layer thickness is 20 um.

Ultimaker S5 FDM Printer

The Ultimaker printers are Fused Deposition Modeling (FDM) printers with dual print heads and heated build platforms. They can accommodate most thermoplastic structural resins including support material. The build volume of the Ultimaker 3 is 215 x 215 x 300 mm, and 330 x 240 x 300 mm for the Ultimaker 5. The minimum layer thickness is 20 um.

Vapourtec R-Series Flow Chemistry System

The Vapourtec flow chemistry R-series system is a flexible, precise, and automatable platform designed for efficient synthesis, reaction optimization, and scale-up. The user-friendly walk-up interface allows all users to leverage the advantages of flow chemistry, including precise temperature control, shortened reaction times, low risk in handling dangerous compounds, and scalability. The Vapourtec system at BioPACIFIC features a range of reactors offering diverse conditions for thermal, photochemical, electrochemical, and heterogeneous reactions. Automated reagent addition Fraction collection triggered by inline analytics including UV-Vis, NMR, and FT-IR FlowIR FT-IR analysis from Mettler Toledo 60 MHz 1H NMR flow-cell based analysis from Magritek – no deuterated solvents necessary! UV-vis analysis with variable wavelength (190–750 nm) Photochemistry supported at 365, 400, and 450 nm wavelengths Electrochemistry with broad electrode compatibility and constant current or voltage modes Temperatures spanning –70 to 250 °C and wide-ranging flow rates (0.02–10 mL/min)