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HSC Cell Imaging Core

University of Utah

Contact Info:

Xiang Wang

30 North 2030 East

Building 585

Salt Lake City, UT 84112

https://cores.utah.edu/cell-imaging/

Grants and Identifiers:

RRID: RRID:SCR_023496

Instrumentation:

Nikon A1 Confocal Laser Microscope

Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]

RRID:SCR_020318

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Nikon A1R Confocal Laser Scanning Microscope

Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]

RRID:SCR_020317

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Olympus FV1000 Confocal Microscope

Next generation imaging system designed for high resolution, confocal observation of both fixed and living cells.FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens.In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photoactivation. [Product Link]

RRID:SCR_020337

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Zeiss LSM 710 Confocal Inverted Microscope

LSM 710 is a confocal is an inverted microscope that enables confocal microscopy for a wide variety of applications. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. [Product Link]

RRID:SCR_018063

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Zeiss LSM 880 with Airyscan Confocal Laser Scanning Microscope

Fully enclosed laser scanning confocal imaging microscopefor heating and environmental control with media perfusion capabilities and CO2 control. It can be used for Live Cell Imaging, FRET and Anisotropy, FRAP and FLIP, FCS, Photoactivation/ -conversion. [Product Link]

RRID:SCR_020925

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Services Provided:

In-vivo Imaging

Scanning 20X Brightfield

Scanning 20X Flourescent

Scanning 40X Brightfield

Scanning 40X Fluorescent

Relevant Publications:

Description:

The HSC Cell Imaging Core offers exceptional services to both internal and external researchers at the University of Utah, as well as customers from various companies. Our core provides access to cutting-edge optical microscopes and imaging analysis software, and our experienced members offer comprehensive training for patrons to use the core or take advantage of our Assisted Imaging service. Additionally, we can offer extensive collaboration for complex projects. With the support of the University and our patrons, the HSC Cell Imaging Core has expanded to six different locations, making it easy for around 300 patrons annually to access our 15+ standard and state-of-the-art microscopes across the campus.