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UArizona Functional Genomics Core

University of Arizona

Contact Info:

Matthew Kaplan

1657 East Helen Street

University of Arizona

Tucson, AZ 85719

http://fgc.arizona.edu

Grants and Identifiers:

RRID: RRID:SCR_023433

Instrumentation:

Services Provided:

Assay Development

Assays and Measurements

Automated Immunoassay Platforms

Automated Liquid Handling

Cell Culture

Cell Imaging

Confocal Microscopy

Drug Discovery

End-to-end Service

Fluorescence Spectroscopy

Genomics

High Content Screening

High-throughput Screening

Image Aquisition

Immunoassay

Immunofluorescence

Library Services

Live Cell Imaging

Optical Imaging

Pipeline Development

Plate Reader

Project Management

SiRNA Screening (human, Genome Wide)

Tissue Culture

Training

Whole Slide Imaging

Relevant Publications:

Description:

The Functional Genomics Core’s mission is to provide researchers at the University of Arizona with the capacity to run high-throughput compound, RNAi, and deletion library based screens for the purpose of network analysis, drug and/or target discovery. We will work with you to develop an assay, carryout a screen and/or analyze the data. The equipment in the laboratory is also available for general (low-throughput) use. Our facility is setup in a modular fashion to ensure flexibility. We will develop a custom pipeline to meet your research goals. Our Biomek FX liquid handling workstation with an integrated Liconic shaker incubator (temperature, humidity, and CO2 controlled) can be used to setup and grow most cell types in 96 well plates. This robot is equipped to add RNAi constructs, drugs or other stimuli to each plate. The Biomek FX can also be used to perform high-throughput biochemical assays. Cell based assays can be carried out using either the Operetta CLS high-content analysis system or the CLARIOstar plus. Both instruments have powerful, but easy to use software for data analysis.