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UArizona Flow Cytometry and Human Immune Monitoring Shared Resource

University of Arizona

Contact Info:

Mark Curry

1515 N Campbell Ave

Tucson, AZ 85724

https://www.cores.arizona.edu/facilities/flowcytometry

Grants and Identifiers:

RRID: RRID:SCR_023432

Instrumentation:

Services Provided:

Cell Sorting

FACS Cell Sorting

Flow Cytometric Analysis

Flow Cytometry Data Analysis

Imaging Flow Cytometry

Imaging Flow Cytomtry

Multi-color Flow Cytometry

Spectral Flow Cytometry

Relevant Publications:

1.) Seka DJ, Schulz AK, Thaker TM, Tomasiak TM (2024 May 22). The N-terminal signature motif on the transporter MCT1 is critical for CD147-mediated trafficking The Journal of Biological Chemistry, 300(6), 107333. . PMCID: 11176948.


Description:

Flow Cytometry Our state-of-the-art flow cytometry instrumentation is available for data acquisition, analysis, and cell sorting, and the technical expertise to interpret results, and develop methods. We offer information about new techniques, and applications of flow cytometry through workshops and seminars, and provide training to interested facility users who wish to run their own samples. Individual consultation services are available to discuss the specifics of each project. Flow cytometry is a powerful tool that measures the functional and structural characteristics of heterogeneous mixtures of cells and particles in suspension based on their ability to scatter light. The cell sorting function separates these cells physically into their different classes. Researchers have the capability to analyze and sort cells by differences in physiology, metabolism, morphology and other characteristics. The ability to distinguish different cell types is limited only by the ability to attach specific fluorescent markers to the cells. The Flow Cytometry component is jointly supported by RII and the UACC. Human Immune Monitoring The Human Immune Monitoring Facility provides comprehensive immune profiling, sample preparation, and clinical trial support. Our mission is to provide technical expertise to clinical investigators and research scientists who want to characterize the immune system by offering up-to-date immune monitoring services for clinical and translational studies. Our services include multispectral flow cytometry, metabolic analysis, blood services such as PBMC isolation and serum/plasma extraction, and a full spectrum of immunological services. We also offer custom assays upon request and will work with interested investigators to help get their projects up and running. Biobanking services are also available upon request. We offer training, assisted, unassisted, and technician operated modalities for all our instruments and will meet investigators at their desired level of engagement. All services are available during regular business hours (M-F 8am - 4pm) with 24 hour access to select instruments arriving in the near future. The Human Immune Monitoring component is supported by the UACC and the University of Arizona Personalized Defense Strategic Initiative.