Centre for Genomic Regulation

Contact Info:

Òscar Fornas

88 Dr. Aiguader St.

Barcelona

https://www.crg.eu/en/programmes-groups/crgupf-flow-cytometry-unit

Grants and Identifiers:

RRID: RRID:SCR_023372

Instrumentation:

Services Provided:

Relevant Publications:

Description:

Flow cytometry studies optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of parameters of individual particles simultaneously, quickly and on a large number of individualized particles in suspension. Flow cytometers use lasers as a source of light excitement; therefore, it must be possible to mark the particles with one or more fluorescent substances. Information is also collected regarding the size and structural complexity of each particle. This multiparameter study of each particle enables us to analyse subpopulations in complex samples through electronic sorting. Some cytometers are also equipped with sorting and collection modules for particles of interest. The most important applications of flow cytometry include those relating to the study of cell surface receptors, nuclear and cytoplasmic antigens, DNA content, enzyme activity, cell integrity and membrane permeability and calcium flows. The Unit currently hosts five analyzers and two sorters, and is therewith the largest Becton Dickinson site in Spain. The sorters are operated by the facility personnel, who also support the users in experimental design and data analysis. The analyzers are operated by the users themselves, after they have been trained. The facility personnel helps with the experimental design and supervises and monitors the experiments when necessary.