Max Planck Institute for Molecular Genetics
Contact Info:
Jan Peychl/Britta Schroth-Diez
Max Placnk Institute of Molecular Cell Biology and Genetics
Pfotenhauerstr. 108
Dresden 01307
Grants and Identifiers:
RRID: RRID:SCR_023307
Instrumentation:
Olympus FluoView 300 Confocal Microscope
Confocal microscopy can improve conventional fluorescence images by recording fluorescence generated from the focal plane within the sample, while rejecting all other light coming from above or below the focal plane. The efficient point-scan/pinhole-detection confocal optics of the FluoView systems virtually eliminate out of focus light to produce high-contrast images with superb resolution. The FluoView systems are fully integrated workstations that incorporate user-friendly image acquisition and image analysis software with high-resolution confocal optics that require no user alignment. An , Windows-based graphic user interface allows new users to quickly generate images in various scan modes, such as XY, XZ, XT, XYZ, XYT, and XYZT. Standard image formats, including TIFF and AVI, permit easy, direct export of FluoView images to off-line analysis packages. XY scanning is performed with a pair of galvanometric mirrors, yielding a wide scanning range to cover up to a field number of 20. The optical zoom (up to 10x magnification) can be performed by narrowing the scanning range while maintaining the maximum pixel resolution of up to 2048 x 2048 pixels. [Product Link]
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Zeiss Axio Vert series Axiovert 200 inverted microscope
Inverted microscope that is used for the examination of cell and tissue culture of sediments in culture flasks, Petri dishes, microtiter plates, etc. in transmitted and reflected light. It hass attachment possibilities for various tools (micromanipulation), different light sources, temperature control devices. [Product Link]
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Zeiss Lightsheet Z.1 Lightsheet Fluorescence Microscope
Light sheet fluorescence microscope for the imaging of large cleared specimens. Lightsheet Z.1 with Clr Plan-Apochromat 20x/1.0 Corr nd=1.38 is used to perform experiments with tissue cleared by Scale medium (Hama et al, Nat Neurosci. 2011), which has a refractive index of n=1.38. [Product Link]
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Zeiss LSM 780 Confocal Laser Scanning Microscope
Laser Scanning Microscopes (LSM) for high resolution imaging of biological or material samples. The compact scanning module can be fitted to an inverted (Axio Observer.Z1 RP or SP) or upright (Axio Imager.Z2, Axio Imager.M2 or Axio Examiner) microscope. [Product Link]
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Zeiss LSM 880 with Airyscan Confocal Laser Scanning Microscope
Fully enclosed laser scanning confocal imaging microscopefor heating and environmental control with media perfusion capabilities and CO2 control. It can be used for Live Cell Imaging, FRET and Anisotropy, FRAP and FLIP, FCS, Photoactivation/ -conversion. [Product Link]
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Zeiss PALM MicroBeam PALM
PALM MicroBeam scanning electron microscope for isolating uncontaminated source material simple. [Product Link]
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Services Provided:
Confocal Microscope With FCS/FLIM Capabilities
Confocal/ Super Resolution Microscope
Correlative Light Electron Microscopy
Fluorescence Microscope With Optical Sectioning Capability
Multiphoton/ Harmonic Generation Microscope
Spinning Disk Confocal Imaging
Relevant Publications:
Description:
The mission of the MPI-CBG Light Microscopy Facility (MPI-CBG LMF) is to provide 24/7 access to state-of-the-art light microscopy techniques and instruments as well as to follow future trends. We assist through consultation, training, and project collaborations. The LMF advises users during design and execution of their imaging projects. We provide support with selecting the most suitable imaging techniques, microscope systems, and setting up imaging experiments. We offer our expertise in imaging techniques, such as Spinning disk confocal microscopy Laser scanning confocal microscopy 2-photon microscopy Light sheet microscopy Wide-field microscopy Total internal reflection fluorescence (TIRF) microscopy Laser capture microdissection Extended resolution microscopy Quantitative phase imaging Live cell and organoid imaging Photomanipulation Furthermore, the LMF is constantly exploring new commercial technologies that can support the research mission of the MPI-CBG. We are also involved in multiple outreach and educational activities in Dresden, Germany and beyond.
