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UArizona Imaging Cores - Electron

University of Arizona

Contact Info:

Paula M Tonino

1333 N Martin Ave.

Life Sciences North

Tucson, AZ 85719

https://research.arizona.edu/facilities/search/imaging-cores-electron-life-sciences-north

Grants and Identifiers:

RRID: RRID:SCR_023279

Instrumentation:

(Equipment No Longer Active At this Facility)

Reichert-Jung Ultracut E Microtome

Ultracut E microtome is designed for extremely precise cutting, and achieves consistent high quality ultra thin sections for electron microscopy, or semi-thin sections for light microscopy. It features a continuous feed range from 0 to 2.5,?m and has adjustable cutting speeds of 0.1 to 50 mm/sec. Comes with a control box with power cord, a microtome sectioning unit, and a microscope assembly with AO Stereostar 570 zoom body (0.7X - 4.2X) with attached 10X eyepieces. It has a 6V, 10W halogen illumination system. [Product Link]

RRID:SCR_020426

cite this instrument

Reichert-Jung Ultracut E Ultramicrotome

The Reichert-Jung Ultracut E Ultramicrotome is used for cutting thick and ultrathin sections, on either glass or diamond knifes, for TEM or SEM

(Equipment No Longer Active At this Facility)

Electron Microscopy Sciences EMS100X Glow Discharge

The EMS100X produces a plasma current of 0 - 50 mA with Positive or Negative Polarity with 60 mm dia. Aluminium electrode.

EMS 100X Glow Discharge

The EMS 100X produce a plasma current of 0 - 50 mA with Positive or Negative Polarity with 60 mm dia. Aluminium electrode.

(Equipment No Longer Active At this Facility)

FEI FEI Tecnai G2 Spirit BT TEM

The FEI Tecnai G2 Spirit BT transmission electron microscope is a general-purpose, high resolution instrument that enables the ultrastructural evaluation of biological and non-biological samples with bright-field, dark- field and diffraction modes of operation.

FEI Tecnai G2 Spirit BT TEM

The FEI Tecnai G2 Spirit BT transmission electron microscope is a general-purpose, high resolution instrument that enables the ultrastructural evaluation of biological and non-biological samples with bright-field, dark- field and diffraction modes of operation.

(Equipment No Longer Active At this Facility)

Leica Leica Ultracut UCT Ultramicrotome

The Leica Ultracut UCT Ultramicrotome is used for cutting thick and ultrathin sections, on either glass or diamond knifes, for TEM or SEM.

Leica Ultracut UCT Ultramicrotome

The Leica Ultracut UCT Ultramicrotome is used for cutting thick and ultrathin sections, on either glass or diamond knifes, for TEM or SEM.

RMC Ultramicrotome PowerTome PT XL

The PTXL model of the RMC PowerTome series. RMC PowerTomes – ultramicrotomes for the preparation of ultra- and semi-thin sections for electron microscopy with patented Power Drive® technology. Mechanical stroke force particularly advantageous for cutting hard and large specimen. Automatic sectioning range: 5nm to 10µm. With digital section counter and feed totalizer. Control of the PTXL via control unit.

Services Provided:

Data Analysis And Interpretation

Electron Microscopy

Image Aquisition

Image Processing And Analysis

Immuno Electron Microscopy

In Situ Hybridization

Microscopy

Microtomy/Electron

Microtomy/Light

Negative Staining For TEM

Protocol Development

Sample Preparation

Staining

Tissue Embed

Training

Transmission Electron Microscopy

Relevant Publications:

1.) Hurtado KA, Schnellmann RG (2024). Mitophagy regulates mitochondrial number following pharmacological induction of mitochondrial biogenesis in renal proximal tubule cells. Frontiers in pharmacology, 15(), 1344075. . PMID: 38375036.


2.) Barefield DY, Tonino P, Woulfe KC, Rahmanseresht S, O'Leary TS, Burnham HV, Wasserstrom JA, Kirk JA, Previs MJ, Granzier HL, McNally EM (2023 Dec 19). Myosin-binding protein H-like regulates myosin-binding protein distribution and function in atrial cardiomyocytes. Proceedings of the National Academy of Sciences of the United States of America, 120(51), e2314920120. . PMID: 38091294.


3.) Hurtado KA, Janda J, Schnellmann RG (2023 Dec). Lasmiditan restores mitochondrial quality control mechanisms and accelerates renal recovery after ischemia-reperfusion injury. Biochemical pharmacology, 218(), 115855. . PMID: 37866804.


4.) Thota LNR, Lopez Rosales JE, Placencia I, Zemskov EA, Tonino P, Michael AN, Black SM, Chignalia AZ (2023 Sep 26). The Pulmonary Endothelial Glycocalyx Modifications in Glypican 1 Knockout Mice Do Not Affect Lung Endothelial Function in Physiological Conditions. International journal of molecular sciences, 24(19), . . PMID: 37834029.


5.) Lindqvist J, Kolb J, de Winter J, Tonino P, Hourani Z, Labeit S, Ottenheijm C, Granzier H (2022 Jul 23). Removal of MuRF1 Increases Muscle Mass in Nemaline Myopathy Models, but Does Not Provide Functional Benefits. International journal of molecular sciences, 23(15), . . ID: 35897687.


6.) Biquand A, Spinozzi S, Tonino P, Cosette J, Strom J, Elbeck Z, Knöll R, Granzier H, Lostal W, Richard I (2021 Sep 15). Titin M-line insertion sequence 7 is required for proper cardiac function in mice. Journal of cell science, 134(18), . . ID: 34401916.


7.) Roxas JL, Ramamurthy S, Cocchi K, Rutins I, Harishankar A, Agellon A, Wilbur JS, Sylejmani G, Vedantam G, Viswanathan VK (2022 Jan-Dec). Enteropathogenic Escherichia coli regulates host-cell mitochondrial morphology. Gut microbes, 14(1), 2143224. . ID: 36476073.


Description:

The Imaging Cores - Electron provides full Biological Transmission Electron Microscopy (TEM) related research, consultation, and imaging services to faculty, scientists and students at the University of Arizona campus, external research institutions and industrial community.