University of Colorado Anschutz Medical Campus
Cassandra Levens
13001 E 17th Pl
Aurora, CO 80045
https://medschool.cuanschutz.edu/rheumatology/research/research-services/gnotobiotic-core-service-center
RRID: RRID:SCR_023245
Allentown Sentry SPP Rack
Sealed Positive Pressure Mouse Caging
Class Biologically Clean 3-ft flexible film isolators
Class Biologically Clean 5-ft flexible film isolators
Animal Husbandry
Colony Management
Gnotobiotic Mice
1.) Parker KD, Maurya AK, Ibrahim H, Rao S, Hove PR, Kumar D, Kant R, Raina B, Agarwal R, Kuhn KA, Raina K, Ryan EP (2021 Feb 3). Dietary Rice Bran-Modified Human Gut Microbial Consortia Confers Protection against Colon Carcinogenesis Following Fecal Transfaunation. Biomedicines, 9(2), . . ID: 33546192.
2.) Lee JS, Wang RX, Goldberg MS, Clifford GP, Kao DJ, Colgan SP (2020 Jun 26). Microbiota-Sourced Purines Support Wound Healing and Mucous Barrier Function. iScience, 23(6), 101226. . ID: 32554188.
3.) Wang RX, Lee JS, Campbell EL, Colgan SP (2020 May 26). Microbiota-derived butyrate dynamically regulates intestinal homeostasis through regulation of actin-associated protein synaptopodin. Proceedings of the National Academy of Sciences of the United States of America, 117(21), 11648-11657. . ID: 32398370.
4.) Rodrigues M, McBride SW, Hullahalli K, Palmer KL, Duerkop BA (2019 Nov). Conjugative Delivery of CRISPR-Cas9 for the Selective Depletion of Antibiotic-Resistant Enterococci. Antimicrobial agents and chemotherapy, 63(11), . . ID: 31527030.
5.) Price VJ, McBride SW, Hullahalli K, Chatterjee A, Duerkop BA, Palmer KL (2019 Jul 24). Enterococcus faecalis CRISPR-Cas Is a Robust Barrier to Conjugative Antibiotic Resistance Dissemination in the Murine Intestine. mSphere, 4(4), . . ID: 31341074.
6.) Soderborg TK, Clark SE, Mulligan CE, Janssen RC, Babcock L, Ir D, Young B, Krebs NF, Lemas DJ, Johnson LK, Weir T, Lenz LL, Frank DN, Hernandez TL, Kuhn KA, D (2019 Jul 1). Author Correction: The gut microbiota in infants of obese mothers increases inflammation and susceptibility to NAFLD. Nature communications, 10(1), 2965. . ID: 31263097.
7.) Chatterjee A, Johnson CN, Luong P, Hullahalli K, McBride SW, Schubert AM, Palmer KL, Carlson PE Jr, Duerkop BA (2019 Jun). Bacteriophage Resistance Alters Antibiotic-Mediated Intestinal Expansion of Enterococci. Infection and immunity, 87(6), . . ID: 30936157.
8.) Li SX, Sen S, Schneider JM, Xiong KN, Nusbacher NM, Moreno-Huizar N, Shaffer M, Armstrong AJS, Severs E, Kuhn K, Neff CP, McCarter M, Campbell T, Lozupone CA, Palmer BE (2019 Apr). Gut microbiota from high-risk men who have sex with men drive immune activation in gnotobiotic mice and in vitro HIV infection. PLoS pathogens, 15(4), e1007611. . ID: 30947289.
9.) Soderborg TK, Clark SE, Mulligan CE, Janssen RC, Babcock L, Ir D, Young B, Krebs N, Lemas DJ, Johnson LK, Weir T, Lenz LL, Frank DN, Hernandez TL, Kuhn KA, D (2018 Oct 26). The gut microbiota in infants of obese mothers increases inflammation and susceptibility to NAFLD. Nature communications, 9(1), 4462. . ID: 30367045.
10.) Alexeev EE, Lanis JM, Kao DJ, Campbell EL, Kelly CJ, Battista KD, Gerich ME, Jenkins BR, Walk ST, Kominsky DJ, Colgan SP (2018 May). Microbiota-Derived Indole Metabolites Promote Human and Murine Intestinal Homeostasis through Regulation of Interleukin-10 Receptor. The American journal of pathology, 188(5), 1183-1194. . ID: 29454749.
11.) Kuhn KA, Schulz HM, Regner EH, Severs EL, Hendrickson JD, Mehta G, Whitney AK, Ir D, Ohri N, Robertson CE, Frank DN, Campbell EL, Colgan SP (2018 Mar). Bacteroidales recruit IL-6-producing intraepithelial lymphocytes in the colon to promote barrier integrity. Mucosal immunology, 11(2), 357-368. . ID: 28812548.
12.) Zheng L, Kelly CJ, Battista KD, Schaefer R, Lanis JM, Alexeev EE, Wang RX, Onyiah JC, Kominsky DJ, Colgan SP (2017 Oct 15). Microbial-Derived Butyrate Promotes Epithelial Barrier Function through IL-10 Receptor-Dependent Repression of Claudin-2. Journal of immunology (Baltimore, Md. : 1950), 199(8), 2976-2984. . ID: 28893958.
13.) Kelly CJ, Zheng L, Campbell EL, Saeedi B, Scholz CC, Bayless AJ, Wilson KE, Glover LE, Kominsky DJ, Magnuson A, Weir TL, Ehrentraut SF, Pickel C, Kuhn KA, Lanis JM, Nguyen V, Taylor CT, Colgan SP (2015 May 13). Crosstalk between Microbiota-Derived Short-Chain Fatty Acids and Intestinal Epithelial HIF Augments Tissue Barrier Function. Cell host & microbe, 17(5), 662-71. . ID: 25865369.
14.) Trikha SRJ, Lee DM, Ecton KE, Wrigley SD, Vazquez AR, Litwin NS, Thomas KN, Wei Y, Battson ML, Johnson SA, Kuhn KA, Colgan SP, Gentile CL, Weir TL (2021 Jan-Dec). Transplantation of an obesity-associated human gut microbiota to mice induces vascular dysfunction and glucose intolerance. Gut microbes, 13(1), 1940791. . ID: 34313540.
The UCD AMC gnotobiotic core provides investigators germ free (axenic/sterile) and gnotobiotic (flora-defined) mice. We provide a space in which investigators can complete their own studies or hire the core to complete experimental manipulations. Our core facility includes 12 large soft-sided vinyl isolators for breeding and 8 small soft-sided vinyl isolators for experiments. We also have the option between using the Allentown positive pressure racks and static cage for experimental use.
