McLaughlin Research Institute

Contact Info:

Teresa Gunn

1520 23rd St South

McLaughlin Research Institute

Great Falls, MT 59405

https://www.mclaughlinresearch.org/gemma-core-2

Grants and Identifiers:

RRID: RRID:SCR_023108

1P20GM152335-01

Instrumentation:

Services Provided:

Assisted Reproductive Technologies (Rodent IVF, ICSI)

CRISPR Injection Services

CRISPR/Cas Project Design

Embryo Cryopreservation & Recovery Services

ES Cell Injection

Fresh/Frozen Sperm IVF

Transgenics

Relevant Publications:

1.) Dennaoui R, Rädler PD, Wicker MN, Vistisen K, Ferraiuolo RM, Triplett AA, Shrestha H, Liner TA, Manthey KC, Rui H, Cardiff RD, Gunn TM, Perou CM, Wagner KU (2025 Jul 7). Overexpression of TSG101 causes the development of adenosquamous mammary carcinoma. Breast cancer research : BCR, 27(1), 126. . ID: 40624726.

2.) Walker WP, Ratz-Mitchem ML, Wagner KU, Gunn TM (2025 May 28). Neuronal Deletion of Tumor Susceptibility Gene 101 (Tsg101) Causes Rapid Apoptotic Loss of Hippocampal CA3 Neurons Biomolecules, 15(6), 786. . PMCID: 12191344.

3.) Ratz-Mitchem ML, Leary G, Grindeland A, Silvius D, Guter J, Kavanaugh MP, Gunn TM (2023 Dec). Generation and characterization of a knock-in mouse model for spastic tetraplegia, thin corpus callosum, and progressive microcephaly (SPATCCM). Mammalian genome : official journal of the International Mammalian Genome Society, 34(4), 572-585. . ID: 37642681.

4.) Ratz ML, Leary G, Grindeland A, Silvius D, Guter J, Kavanaugh MP, Gunn TM (2023 Apr 24). Generation and characterization of a knock-in mouse model for Spastic Tetraplegia, Thin Corpus Callosum, and Progressive Microcephaly (SPATCCM). Research square, (), . . ID: 37162879.

5.) Kong JH, Young CB, Pusapati GV, Espinoza FH, Patel CB, Beckert F, Ho S, Patel BB, Gabriel GC, Aravind L, Bazan JF, Gunn TM, Lo CW, Rohatgi R (2021 Oct 1). Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength. Development (Cambridge, England), 148(19), . . ID: 34486668.

6.) Kong JH, Young CB, Pusapati GV, Patel CB, Ho S, Krishnan A, Lin JI, Devine W, Moreau de Bellaing A, Athni TS, Aravind L, Gunn TM, Lo CW, Rohatgi R (2020 Nov 23). A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development. Developmental cell, 55(4), 432-449.e12. . ID: 32966817.

Description:

This facility provides services necessary for investigators to create, rederive or cryopreserve mouse models. Specific services offered include: CRISPR/Cas9-mediated gene editing (experimental design service and/or consulting also provided); pronuclear Injection; ES Cell Injection; In Vitro Fertilization; rederivation of mouse lines to specific pathogen free status; embryo and sperm cryopreservation and recovery.