University of Michigan–Ann Arbor
Ashu Tripathi
210 Washtenaw Ave.
Ann Arbor, MI 48109
https://www.lsi.umich.edu/science/centers-technologies/natural-products-discovery-core
RRID: RRID:SCR_023105
Agilent 6545 LC/Q-TOF
LF/Q-TOF is used for untargeted sample analysis and enables broad screening, comprehensive profiling, and identification of unknowns. [Product Link]
RRID:SCR_019429
Agilent RapidFire 365 High-Throughput Mass Spectrometry System
Mass Spectrometry System replaces HPLC with automated solid phase extraction SPE to increase throughput of LC MS analyses. [Product Link]
RRID:SCR_019530
Gilson GX-271 HPLC Liquid Handler System
HPLC liquid handler with midsize bed capacity and fraction collector. It has maximum flow rates up to 200 mL per min, injection loop volumes of 2 uL to 25 mL, and max sample mass of 15 grams per injection. [Product Link]
RRID:SCR_018579
Biotage Selekt Flash Chromatography
Biotage SPE Dry (Microplate system)
Biotage V10 Touch with Gilson Handler
Bruker 800 Mhz with CryoPorbe
(Equipment No Longer Active At this Facility)
Shimadzu Shimadzu LCMS-2020
Consultation
Deconvolution
Drug Discovery
Genomics
Mass Spectrometry
Metabolomics
Natural Product Analysis
1.) Zhuang Y, Yang F, Menon A, Song JM, Espinoza RV, Schultz PJ, Garner AL, Tripathi A (2023 Jul 28). An ECD and NMR/DP4+ Computational Pipeline for Structure Revision and Elucidation of Diphenazine-Based Natural Products. Journal of natural products, 86(7), 1801-1814. doi: 10.1021/acs.jnatprod.3c00306. ID: 37463274.
2.) Mahjour B, Zhang R, Shen Y, McGrath A, Zhao R, Mohamed OG, Lin Y, Zhang Z, Douthwaite JL, Tripathi A, Cernak T (2023 Jul 3). Rapid planning and analysis of high-throughput experiment arrays for reaction discovery. Nature communications, 14(1), 3924. . ID: 37400469.
3.) Otify AM, Mohamed OG, El-Amier YA, Saber FR, Tripathi A, Younis IY (2023 Mar 9). Bioherbicidal Activity and Metabolic Profiling of Allelopathic Metabolites of Three Cassia species using UPLC-qTOF-MS/MS and Molecular Networking. Metabolomics : Official journal of the Metabolomic Society, 19(3), 16. doi: 10.1007/s11306-023-01980-5. ID: 36892715.
4.) Ostrowski MP, La Rosa SL, Kunath BJ, Robertson A, Pereira G, Hagen LH, Varghese NJ, Qiu L, Yao T, Flint G, Li J, McDonald SP, Buttner D, Pudlo NA, Schnizlein MK, Young VB, Brumer H, Schmidt TM, Terrapon N, Lombard V, Henrissat B, Hamaker B, Eloe-Fadrosh EA, Tripathi A, Pope PB, Martens EC (2022 Apr). Mechanistic insights into consumption of the food additive xanthan gum by the human gut microbiota. Nature microbiology, 7(4), 556-569. doi: 10.1038/s41564-022-01093-0. ID: 35365790.
5.) Mohamed OG, Dorandish S, Lindow R, Steltz M, Shoukat I, Shoukat M, Chehade H, Baghdadi S, McAlister-Raeburn M, Kamal A, Abebe D, Ali K, Ivy C, Antonova M, Schultz P, Angell M, Clemans D, Friebe T, Sherman D, Casper AM, Price PA, Tripathi A (2021 Jul 27). Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique. Marine drugs, 19(8), . . ID: 34436264.
6.) Robertson AW, Sandoval J, Mohamed OG, Zhuang Y, Gallagher EE, Schmidt J, Caratelli L, Menon A, Schultz PJ, Torrez RM, Hay CL, Bell BA, Price PA, Garner AL, Tripathi A (2021 Jun 10). Discovery of Surfactins as Inhibitors of MicroRNA Processing Using Cat-ELCCA. ACS medicinal chemistry letters, 12(6), 878-886. . ID: 34141065.
Library of >50,000 NPEs with technology and expertise to develop candidates identified through HTS into unique, bioactive, patentable small molecules.
