Virginia Commonwealth University
Contact Info:
Kalyan Mallempati
1101 E. Marshall St
Sanger Hall Rm. 5-028 Richmond, VA 23298
Richmond, VA 23298
https://research.vcu.edu/cores/genomics/
Grants and Identifiers:
RRID: RRID:SCR_023050
Instrumentation:
10X Genomics Chromium Plus Genetic Analyzer
Genetic analyzer, referred to as Chromium Controller for combining large partition numbers with a diverse barcode library to generate >100,000 barcode-containing partitions. [Product Link]
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Agilent 4150 TapeStation System
This TapeStation System analyzes nucleic acids for up to 16 samples per run. [Product Link]
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Agilent 4200 TapeStation System
TapeStation System is used to analyze DNA and RNA samples for quality control. [Product Link]
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(Equipment No Longer Active At this Facility)
Agilent TapeStation Laptop
TapeStation Laptop is used to standardize data acquisition and analysis. [Product Link]
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Covaris E220evolution Focused-Ultrasonicator
Focused ultrasonicator that enables multi sample, batch preparation, capable of processing wide range of sample types and volumes. [Product Link]
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Illumina MiSeq System
Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]
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Illumina NextSeq 1000 and NextSeq 2000 Sequencing Systems
Benchtop sequencers that allow you to explore new science across variety of current and emerging applications, with higher efficiency and fewer restraints. [Product Link]
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Nanodrop Qubit 3 Fluorometer
Benchtop fluorometer that can be used for the quantitation of DNA, RNA, microRNA, and protein using the highly sensitive and accurate fluorescence-based Qubit quantitation assays. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 3.0 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion Personal Genome Machine (PGM) Sequencer. You can also use the Qubit 3.0 Fluorometer to directly measure the fluorescence of samples or to create new assay(s) using the MyQubit software pre-programmed into the instrument. [Product Link]
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Qiagen QIAcube
DNA/RNA Protein Purification System for automated purification of nucleic acids and proteins in molecular biology applications. [Product Link]
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Sage Science BluePippin system
Used for DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field (100bp ? 50kb).BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination. [Product Link]
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Thermo Scientific™ Nanodrop 2000 microvolume spectrophotometer
Thermo Scientific™ NanoDrop 2000 is a full-spectrum, UV-Vis spectrophotometers that quantifies and assesses purity of DNA, RNA, Protein and more. The NanoDrop 2000 and its series companion 2000c have patented sample retention technology that measure sample volumes as small as 0.5μL. [Product Link]
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(Equipment No Longer Active At this Facility)
10X Genomics 10x genomics visium cytassist
The 10x Genomics Visium CytAssist is a compact, benchtop instrument that enables whole transcriptome spatial profiling by transferring hybridized transcriptomic probes from standard histological slides to specially designed Visium capture slides. It captures a brightfield image of the tissue, aligns it precisely, and then transfers the probes to the Visium slide, allowing for spatial gene expression analysis of fresh-frozen or FFPE tissue sections that have been stained for H&E or immunofluorescence.This process maximizes experimental efficiency and allows for unbiased discovery by leveraging standard histology workflows and enabling pre-screening of tissue sections for their biological relevance
10X genomics Visium Cytassist
The 10x Genomics Visium CytAssist is a compact, benchtop instrument that enables whole transcriptome spatial profiling by transferring hybridized transcriptomic probes from standard histological slides to specially designed Visium capture slides. It captures a brightfield image of the tissue, aligns it precisely, and then transfers the probes to the Visium slide, allowing for spatial gene expression analysis of fresh-frozen or FFPE tissue sections that have been stained for H&E or immunofluorescence. This process maximizes experimental efficiency and allows for unbiased discovery by leveraging standard histology workflows and enabling pre-screening of tissue sections for their biological relevance
Qiagen QIAcuity Digital PCR System
The QIAGEN QIAcuity is a digital PCR (dPCR) system that offers walk-away automation for the complete workflow, including partitioning, thermocycling, and imaging, into a single instrument to provide absolute quantification. It utilizes proprietary microfluidic Nanoplates with numerous partitions for high-precision sample analysis. The system is designed for speed and efficiency, delivering results in approximately 2 hours, and is scalable with different instrument configurations to accommodate various laboratory throughput needs.
Services Provided:
Illumina RNA/DNA NGS Library Construction
Sequencing - Next Generation Sequencing (NGS)
Visium HD Spatial Transcriptomics
Relevant Publications:
Description:
The Genomics Core facility in Richmond VA, maintains a significant portfolio of instrumentation to support advanced sample/library preparation, high-throughput sequencing, real-time PCR and analysis.
