Salk Institute for Biological Studies

Contact Info:

Carolyn O'Conner

http://www.salk.edu/science/core-facilities/flow-cytometry/

Grants and Identifiers:

RRID: RRID:SCR_014839

Instrumentation:

Services Provided:

Relevant Publications:

1.) Lorenzini MH, Balderson B, Sajeev K, Ho AJ, McVicker G (2025 Aug 28). Joint single-cell profiling of Cas9 edits and transcriptomes reveals widespread off-target events and effects on gene expression bioRxiv, (), 2025.02.07.636966. . PMCID: 12407703.

2.) Esparza-Moltó PB, Goswami AV, Bozkurt S, Münch C, Newman LE, Moyzis AG, Rojas GR, Guan D, Jones JR, Gage FH, Shadel GS (2025 Aug 12). ROS-dependent localization of glycolytic enzymes to mitochondria Redox Biology, 86(), 103812. . PMCID: 12359183.

3.) Jaquish A, Phung E, Gong X, Baldominos P, Galván-Peña S, Magill I, Bursulaya I, Marina E, Bertrand K, Chambers C, Muñoz-Rojas AR, Agudo J, Mathis D, Benoist C, Ramanan D (2025 Jul 29). Mammary intraepithelial lymphocytes and intestinal inputs shape T cell dynamics in lactogenesis Nature immunology, 26(8), 1411-1422. . PMCID: 12369998.

4.) Miller CN, Jarrell-Hurtado S, Haag MV, Sara Ye Y, Simenc M, Alvarez-Maldonado P, Behnami S, Zhang L, Swift J, Papikian A, Yu J, Colt K, Ecker JR, Michael TP, Law JA, Busch W (2025 May 5). A single-nuclei transcriptome census of the Arabidopsis maturing root identifies that MYB67 controls phellem cell maturation. Developmental cell, 60(9), 1377-1391.e7. . PMID: 39793584.

5.) Strutzenberg TS, Mann MD, Li X, Shin H, Kelsey J, Aiyer S, Yu J, Gray G, Zhang Z, Shan Z, Zhou B, Zheng Y, Griffin PR, Lyumkis D (2025 Mar 12). Nucleosome Engagement Regulates RORγt Structure and Dynamics bioRxiv, (), 2025.03.10.642251. . PMCID: 11952427.

6.) Ma S, Dahabieh MS, Mann TH, Zhao S, McDonald B, Song WS, Chung HK, Farsakoglu Y, Garcia-Rivera L, Hoffmann FA, Xu S, Du VY, Chen D, Furgiuele J, LaPorta MA, Jacobs E, DeCamp LM, Oswald BM, Sheldon RD, Ellis AE, Liu L, He P, Wang Y, Jang C, Jones RG, Kaech SM (2025 Feb 7). Nutrient-driven histone code determines exhausted CD8(+) T cell fates. Science (New York, N.Y.), 387(6734), eadj3020. . PMID: 39666821.

7.) Xu Z, Kuhlmann-Hogan A, Xu S, Tseng H, Chen D, Tan S, Sun M, Tripple V, Bosenberg M, Miller-Jensen K, Kaech SM (2025 Feb 1). Scavenger Receptor CD36 in Tumor-Associated Macrophages Promotes Cancer Progression by Dampening Type I Interferon Signaling Cancer research, 85(3), 462-476. . PMCID: 11788022.

8.) Xu Z, Kuhlmann-Hogan A, Xu S, Tseng H, Chen D, Tan S, Sun M, Tripple V, Bosenberg M, Miller-Jensen K, Kaech SM (2025 Feb 1). Scavenger Receptor CD36 in Tumor-Associated Macrophages Promotes Cancer Progression by Dampening Type-I IFN Signaling. Cancer research, 85(3), 462-476. . PMID: 39546763.

9.) Cochrane WG, Bare GA, Joyce GF, Horning DP (2024 Oct 22). Cross-chiral exponential amplification of an RNA enzyme Proceedings of the National Academy of Sciences of the United States of America, 121(44), e2413668121. . PMCID: 11536142.

10.) Vallmajo-Martin Q, Ma Z, Srinivasan S, Murali D, Dravis C, Mukund K, Subramaniam S, Wahl GM, Lytle NK (2024 Oct 9). The molecular chronology of mammary epithelial cell fate switching bioRxiv, (), 2024.10.08.617155. . PMCID: 11482796.

11.) Reina J, Vallmajo-Martin Q, Ning J, Michi AN, Yeung K, Wahl GM, Hunter T (2024 Apr 23). LHPP expression in triple-negative breast cancer promotes tumor growth and metastasis by modulating the tumor microenvironment. bioRxiv : the preprint server for biology, (), . . PMID: 38712081.

12.) Labarta-Bajo L, Deng J, Contreras M, Allen NJ (2023 Dec 15). Protocol for the purification and transcriptomic analysis of mouse astrocytes using GFAT. STAR protocols, 4(4), 102599. . PMID: 37742178.

13.) Zhou J, Zhang Z, Wu M, Liu H, Pang Y, Bartlett A, Peng Z, Ding W, Rivkin A, Lagos WN, Williams E, Lee CT, Miyazaki PA, Aldridge A, Zeng Q, Salinda JLA, Claffey N, Liem M, Fitzpatrick C, Boggeman L, Yao Z, Smith KA, Tasic B, Altshul J, Kenworthy MA, Valadon C, Nery JR, Castanon RG, Patne NS, Vu M, Rashid M, Jacobs M, Ito T, Osteen J, Emerson N, Lee J, Cho S, Rink J, Huang HH, Pinto-Duartec A, Dominguez B, Smith JB, O'Connor C, Zeng H, Chen S, Lee KF, Mukamel EA, Jin X, Margarita Behrens M, Ecker (2023 Dec). Brain-wide correspondence of neuronal epigenomics and distant projections. Nature, 624(7991), 355-365. . PMID: 38092919.

14.) Chen HV, Lorenzini MH, Lavalle SN, Sajeev K, Fonseca A, Fiaux PC, Sen A, Luthra I, Ho AJ, Chen AR, Guruvayurappan K, O'Connor C, McVicker G (2023 Apr 6). Deletion mapping of regulatory elements for GATA3 in T cells reveals a distal enhancer involved in allergic diseases. American journal of human genetics, 110(4), 703-714. . PMID: 36990085.

Description:

Core facility dedicated to advancing research projects requiring cell sorting and/or analysis of cell populations by flow cytometry. The core offers training on and access to shared use instrumentation for analytical flow cytometry, services for fluorescence activated cell sorting (FACS) as well as technical consultation and support on experimental design, execution, and data analysis.