University of Rochester Medical Center
Matthew Cochran
601 Elmwood Avenue
Rochester, NY 14642
https://www.urmc.rochester.edu/research/flow-core.aspx
RRID: RRID:SCR_012360
(Equipment No Longer Active At this Facility)
BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
RRID:SCR_019601
Agilent Seahorse XFe96 Lew Zealand
Cellular metabolomics
BD Accuri C6+ Pepe
2 (B, R) laser, 4 fluorescent parameters
BD FACSAriaII Statler
4 (V, B, G, R) lasers, 18 fluorescent parameters
BD FACSAriaII Waldorf
4 (V, B, G, R) lasers, 18 fluorescent parameters
(Equipment No Longer Active At this Facility)
BD LSRII
4 (V,B,G,R) lasers, 18 fluorescent parameters
BD LSRII Animal
4 (V,B,G,R) lasers, 18 fluorescent parameters
BD LSRII Fozzie
4 (V,B,G,R) lasers, 18 fluorescent parameters
BD LSRII Oscar
4 (V,B,G,R) lasers, 18 fluorescent parameters
BD LSR_Fortessa Camilla the Chicken
5 (UV, V, B, YG, R) laser, 18 fluorescent parameter
BD LSR_Fortessa Dr. Teeth
5 (UV, V, B, YG, R) laser, 18 fluorescent parameter
BD Symphony A1 Janice
4 (V, B, YG, R), 16 fluorescent parameters
BioRad S3e Scooter
2 (B, YG) lasers, 4 fluorescent parameters
Cytek Aurora Constantine
4 (UV, V, B, R) laser full spectrum cytometer
Cytek Aurora Sweetums
5 (UV, V, B, YG, R) laser full spectrum cytometer
Luminex Image Stream MarkII Big Bird
2 camera, 10 fluorescent parameter imaging flow cytometer
Malvern Nanosight NS300 Bean Bunny
Small particle detection
Nexcelom Celigo Stinky the Stinkweed
Imaging cytometer
Standard Bio Tools Helios Ludo
Mass Cytometer
Analysis Workstations
Assay Development
Assays and Measurements
Automated Immunoassay Platforms
Cell Imaging
Cell Sorting
Clinical Trial Support
Computational - Application Development
Computational - Bioinformatics
Consultation
Consultations
CyTOF
Cytokine Assays
Data Analysis
Data Analysis And Interpretation
Data Visualization
DNA Analysis
FACS Cell Sorting
Flow Cytometric Analysis
Flow Cytometry Data Analysis
Imaging Flow Cytometry
Imaging Flow Cytomtry
Immune Monitoring
Immunoassay
Light Scattering
Luminex Bead Based Cytokine Assays
Luminex Services
Mass Cytometry
Multi-color Flow Cytometry
Nanosight Nanoparticle Analysis
Protocol Development
RNA Flow Cytometry
Spectral Flow Cytometry
Staining
Training
1.) Burke RM, Dirkx RA Jr, Quijada P, Lighthouse JK, Mohan A, O (2021 May 28). Prevention of Fibrosis and Pathological Cardiac Remodeling by Salinomycin. Circulation research, 128(11), 1663-1678. . ID: 33825488.
2.) Box A, DeLay M, Tighe S, Chittur SV, Bergeron A, Cochran M, Lopez P, Meyer EM, Saluk A, Thornton S, Brundage K (2020 Sep). Evaluating the Effects of Cell Sorting on Gene Expression. Journal of biomolecular techniques : JBT, 31(3), 100-111. . ID: 32982601.
3.) Bhowmick D, Sheridan RTC, Bushnell TP, Spalding KL (2020 Aug). Practical Guidelines for Optimization and Characterization of the Beckman Coulter CytoFLEX™ Platform. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 97(8), 800-810. doi: 10.1002/cyto.a.23998. ID: 32150325.
4.) Cossarizza A, Chang HD, Radbruch A, Acs A, Adam D, Adam-Klages S, Agace WW, Aghaeepour N, Akdis M, Allez M, Almeida LN, Alvisi G, Anderson G, Andrä I, Annunziato F, Anselmo A, Bacher P, Baldari CT, Bari S, Barnaba V, Barros-Martins J, Battistini L, Bauer W, Baumgart S, Baumgarth N, Baumjohann D, Baying B, Bebawy M, Becher B, Beisker W, Benes V, Beyaert R, Blanco A, Boardman DA, Bogdan C, Borger JG, Borsellino G, Boulais PE, Bradford JA, Brenner D, Brinkman RR, Brooks AES, Busch DH, Büscher M, Bu (2019 Oct). Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European journal of immunology, 49(10), 1457-1973. . ID: 31633216.
5.) Hollenbach SJ, Cochran M, Harrington A (2019 Aug). "Provoked" feto-maternal hemorrhage may represent insensible cell exchange in pregnancies from 6 to 22 weeks gestational age. Contraception, 100(2), 142-146. doi: 10.1016/j.contraception.2019.03.051. ID: 30980826.
6.) Stone SL, Peel JN, Scharer CD, Risley CA, Chisolm DA, Schultz MD, Yu B, Ballesteros-Tato A, Wojciechowski W, Mousseau B, Misra RS, Hanidu A, Jiang H, Qi Z, Boss JM, Randall TD, Brodeur SR, Goldrath AW, Weinmann AS, Rosenberg AF, Lund FE (2019 May 21). T-bet Transcription Factor Promotes Antibody-Secreting Cell Differentiation by Limiting the Inflammatory Effects of IFN-γ on B Cells. Immunity, 50(5), 1172-1187.e7. . ID: 31076359.
7.) Zumaquero E, Stone SL, Scharer CD, Jenks SA, Nellore A, Mousseau B, Rosal-Vela A, Botta D, Bradley JE, Wojciechowski W, Ptacek T, Danila MI, Edberg JC, Bridges SL Jr, Kimberly RP, Chatham WW, Schoeb TR, Rosenberg AF, Boss JM, Sanz I, Lund FE (2019 May 15). IFNγ induces epigenetic programming of human T-bet(hi) B cells and promotes TLR7/8 and IL-21 induced differentiation. eLife, 8(), . . ID: 31090539.
8.) Bandyopadhyay G, Huyck HL, Misra RS, Bhattacharya S, Wang Q, Mereness J, Lillis J, Myers JR, Ashton J, Bushnell T, Cochran M, Holden-Wiltse J, Katzman P, Deutsch G, Whitsett JA, Xu Y, Mariani TJ, Pryhuber GS (2018 Oct 1). Dissociation, cellular isolation, and initial molecular characterization of neonatal and pediatric human lung tissues. American journal of physiology. Lung cellular and molecular physiology, 315(4), L576-L583. . ID: 29975103.
9.) Kyle JE, Clair G, Bandyopadhyay G, Misra RS, Zink EM, Bloodsworth KJ, Shukla AK, Du Y, Lillis J, Myers JR, Ashton J, Bushnell T, Cochran M, Deutsch G, Baker ES, Carson JP, Mariani TJ, Xu Y, Whitsett JA, Pryhuber G, Ansong C (2018 Sep 7). Cell type-resolved human lung lipidome reveals cellular cooperation in lung function. Scientific reports, 8(1), 13455. . ID: 30194354.
10.) Cossarizza A, Chang HD, Radbruch A, Akdis M, Andrä I, Annunziato F, Bacher P, Barnaba V, Battistini L, Bauer WM, Baumgart S, Becher B, Beisker W, Berek C, Blanco A, Borsellino G, Boulais PE, Brinkman RR, Büscher M, Busch DH, Bushnell TP, Cao X, Cavani A, Chattopadhyay PK, Cheng Q, Chow S, Clerici M, Cooke A, Cosma A, Cosmi L, Cumano A, Dang VD, Davies D, De Biasi S, Del Zotto G, Della Bella S, Dellabona P, Deniz G, Dessing M, Diefenbach A, Di Santo J, Dieli F, Dolf A, Donnenberg VS, Dörner T, Eh (2017 Oct). Guidelines for the use of flow cytometry and cell sorting in immunological studies. European journal of immunology, 47(10), 1584-1797. . ID: 29023707.
11.) Barsky LW, Black M, Cochran M, Daniel BJ, Davies D, DeLay M, Gardner R, Gregory M, Kunkel D, Lannigan J, Marvin J, Salomon R, Torres C, Walker R (2016 Nov). International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 89(11), 1017-1030. doi: 10.1002/cyto.a.23016. ID: 27813253.
12.) Misra RS, Bhattacharya S, Huyck HL, Wang JC, Slaunwhite CG, Slaunwhite SL, Wightman TR, Secor-Socha S, Misra SK, Bushnell TP, Reynolds AM, Ryan RM, Quataert SA, Pryhuber GS, Mariani TJ (2016 Oct). Flow-based sorting of neonatal lymphocyte populations for transcriptomics analysis. Journal of immunological methods, 437(), 13-20. . ID: 27438473.
13.) Kuzin II, Kates SL, Ju Y, Zhang L, Rahimi H, Wojciechowski W, Bernstein SH, Burack R, Schwarz EM, Bottaro A (2016 Jul). Increased numbers of CD23(+) CD21(hi) Bin-like B cells in human reactive and rheumatoid arthritis lymph nodes. European journal of immunology, 46(7), 1752-7. . ID: 27105894.
14.) Kingsley PD, Greenfest-Allen E, Frame JM, Bushnell TP, Malik J, McGrath KE, Stoeckert CJ, Palis J (2013 Feb 7). Ontogeny of erythroid gene expression. Blood, 121(6), e5-e13. . ID: 23243273.
15.) León B, Ballesteros-Tato A, Misra RS, Wojciechowski W, Lund FE (2012 Jun). Unraveling effector functions of B cells during infection: the hidden world beyond antibody production. Infectious disorders drug targets, 12(3), 213-21. . ID: 22394173.
16.) Hilchey SP, Rosenberg AF, Hyrien O, Secor-Socha S, Cochran MR, Brady MT, Wang JC, Sanz I, Burack WR, Quataert SA, Bernstein SH (2011 Sep 29). Follicular lymphoma tumor-infiltrating T-helper (T(H)) cells have the same polyfunctional potential as normal nodal T(H) cells despite skewed differentiation. Blood, 118(13), 3591-602. . ID: 21821704.
17.) Williams MA, Bauer S, Lu W, Guo J, Walter S, Bushnell TP, Lillehoj EP, Georas SN (2010). Deletion of the mucin-like molecule muc1 enhances dendritic cell activation in response to toll-like receptor ligands. Journal of innate immunity, 2(2), 123-43. . ID: 20375631.
18.) Wojciechowski W, Harris DP, Sprague F, Mousseau B, Makris M, Kusser K, Honjo T, Mohrs K, Mohrs M, Randall T, Lund FE (2009 Mar 20). Cytokine-producing effector B cells regulate type 2 immunity to H. polygyrus. Immunity, 30(3), 421-33. . ID: 19249230.
19.) Wang JC, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA (2009 Feb 28). An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform. Journal of immunological methods, 341(1), 106-16. . ID: 19049809.
20.) McGrath KE, Bushnell TP, Palis J (2008 Jul 31). Multispectral imaging of hematopoietic cells: where flow meets morphology. Journal of immunological methods, 336(2), 91-7. . ID: 18539294.
21.) Ryan EP, Bushnell TP, Friedman AE, Rahman I, Phipps RP (2008 Mar). Cyclooxygenase-2 independent effects of cyclooxygenase-2 inhibitors on oxidative stress and intracellular glutathione content in normal and malignant human B-cells. Cancer immunology, immunotherapy : CII, 57(3), 347-58. . ID: 17668203.
22.) McGrath KE, Kingsley PD, Koniski AD, Porter RL, Bushnell TP, Palis J (2008 Feb 15). Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus. Blood, 111(4), 2409-17. . ID: 18032705.
23.) Neff-LaFord H, Teske S, Bushnell TP, Lawrence BP (2007 Jul 1). Aryl hydrocarbon receptor activation during influenza virus infection unveils a novel pathway of IFN-gamma production by phagocytic cells. Journal of immunology (Baltimore, Md. : 1950), 179(1), 247-55. . ID: 17579044.
24.) Li H, Wojciechowski W, Dell (2006 Sep 15). IFN-gamma and T-bet expression in human dendritic cells from normal donors and cancer patients is controlled through mechanisms involving ERK-1/2-dependent and IL-12-independent pathways. Journal of immunology (Baltimore, Md. : 1950), 177(6), 3554-63. . ID: 16951314.
25.) Kingsley PD, Malik J, Emerson RL, Bushnell TP, McGrath KE, Bloedorn LA, Bulger M, Palis J (2006 Feb 15). "Maturational" globin switching in primary primitive erythroid cells. Blood, 107(4), 1665-72. . ID: 16263786.
26.) Wojciechowski W, Li H, Marshall S, Dell (2005 Jun 15). Enhanced expression of CD20 in human tumor B cells is controlled through ERK-dependent mechanisms. Journal of immunology (Baltimore, Md. : 1950), 174(12), 7859-68. . ID: 15944291.
27.) Xing L, Bushnell TP, Carlson L, Tai Z, Tondravi M, Siebenlist U, Young F, Boyce BF (2002 Jul). NF-kappaB p50 and p52 expression is not required for RANK-expressing osteoclast progenitor formation but is essential for RANK- and cytokine-mediated osteoclastogenesis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 17(7), 1200-10. . ID: 12096833.
The URMC Flow Cytometry Resource is equipped with a wide array of instrumentation that is rarely matched the world over. This includes 8 traditional analytical cytometers, two full spectrum cytometers, 3 cell sorters, 2 imaging cytometers, a small(nano) particle detection system, a Metabolomics instrument, a BioPlex200, and a mass cytometer.
