National Institute on Drug Abuse

Contact Info:

Christopher Richie

251 Bayview Blvd

Suite 200 Rm 06A729

Baltimore, MD 21224

https://irp.drugabuse.gov/organization/core-facilities/genetic-engineering/

Grants and Identifiers:

RRID: RRID:SCR_022969

Instrumentation:

Services Provided:

Cell Culture

Cloning

Consultation

CRISPR

Digital PCR

DNA Isolation

Viral Vectors

Relevant Publications:

1.) Rich JA, Gurung S, Coates-Park S, Liu Y, Govil A, Stetler-Stevenson WG, Peeney D (2024 Dec 12). Protocol to study secretome interactions using extracellular proximity labeling STAR Protocols, 5(4), 103509. . PMCID: 11699400.

2.) Peeney D, Gurung S, Rich JA, Coates-Park S, Liu Y, Toor J, Jones J, Richie CT, Jenkins LM, Stetler-Stevenson WG (2024 Oct 4). Mapping Extracellular Protein-Protein Interactions Using Extracellular Proximity Labeling (ePL). Journal of proteome research, 23(10), 4715-4728. . PMID: 39238192.

3.) Solomon MG, Nennig SE, Cotton MR, Whiting KE, Fulenwider HD, Schank JR (2024 Aug 27). Neurokinin-1 receptors in the nucleus accumbens shell influence sensitivity to social defeat stress and stress-induced alcohol consumption in male mice Addiction neuroscience, 13(), 100174. . PMCID: 11720327.

4.) Peeney D, Gurung S, Rich JA, Coates-Park S, Liu Y, Toor J, Jones J, Richie CT, Jenkins LM, Stetler-Stevenson WG (2024 Jan 15). Extracellular Proximity Labeling Reveals an Expanded Interactome for the Matrisome Protein TIMP2 Research Square, (), rs.3.rs-3857263. . PMCID: 10836090.

5.) Belilos A, Gray C, Sanders C, Black D, Mays E, Richie C, Sengupta A, Hake H, Francis TC (2023 Dec 26). Nucleus accumbens local circuit for cue-dependent aversive learning. Cell reports, 42(12), 113488. . PMID: 37995189.

6.) Belilos A, Gray C, Sanders C, Black D, Mays E, Richie CT, Sengupta A, Hake HS, Francis TC (2023 Oct 9). Nucleus Accumbens Local Circuit for Cue-Dependent Aversive Learning. bioRxiv : the preprint server for biology, (), . . PMID: 36798245.

7.) Bossert JM, Mejias-Aponte CA, Saunders T, Altidor L, Emery M, Fredriksson I, Batista A, Claypool SM, Caldwell KE, Reiner DJ, Chow JJ, Foltz M, Kumar V, Seasholtz A, Hughes E, Filipiak W, Harvey BK, Richie CT, Vautier F, Gomez JL, Michaelides M, Kieffer BL, Watson SJ, Akil H, Shaham Y (2023 Mar 8). Effect of Selective Lesions of Nucleus Accumbens µ-Opioid Receptor-Expressing Cells on Heroin Self-Administration in Male and Female Rats: A Study with Novel Oprm1-Cre Knock-in Rats. The Journal of neuroscience : the official journal of the Society for Neuroscience, 43(10), 1692-1713. . ID: 36717230.

8.) Green BL, Grant RRC, Richie CT, Chatterjee B, Sampaio De Melo M, Barr FG, Pacak K, Agarwal SK, Nilubol N (2022 Jul 1). Novel GLCCI1-BRAF fusion drives kinase signaling in a case of pheochromocytomatosis. European journal of endocrinology, 187(1), 185-196. doi: 10.1530/EJE-21-0797. ID: 35861986.

9.) Owlett LD, Karaahmet B, Le L, Belcher EK, Dionisio-Santos D, Olschowka JA, Elliott MR, O (2022 Feb 7). Gas6 induces inflammation and reduces plaque burden but worsens behavior in a sex-dependent manner in the APP/PS1 model of Alzheimer Journal of neuroinflammation, 19(1), 38. . ID: 35130912.

10.) Garcia-Keller C, Scofield MD, Neuhofer D, Varanasi S, Reeves MT, Hughes B, Anderson E, Richie CT, Mejias-Aponte C, Pickel J, Hope BT, Harvey BK, Cowan CW, Kalivas PW (2020 Oct 28). Relapse-Associated Transient Synaptic Potentiation Requires Integrin-Mediated Activation of Focal Adhesion Kinase and Cofilin in D1-Expressing Neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(44), 8463-8477. . ID: 33051346.

11.) Venniro M, Russell TI, Ramsey LA, Richie CT, Lesscher HMB, Giovanetti SM, Messing RO, Shaham Y (2020 Apr 7). Abstinence-dependent dissociable central amygdala microcircuits control drug craving. Proceedings of the National Academy of Sciences of the United States of America, 117(14), 8126-8134. . ID: 32205443.

12.) Hartman JH, Richie CT, Gordon KL, Mello DF, Castillo P, Zhu A, Wang Y, Hoffer BJ, Sherwood DR, Meyer JN, Harvey BK (2019 Dec). MANF deletion abrogates early larval Caenorhabditis elegans stress response to tunicamycin and Pseudomonas aeruginosa. European journal of cell biology, 98(5), 151043. . ID: 31138438.

13.) Campbell LA, Richie CT, Maggirwar NS, Harvey BK (2019 Dec). Cas9 Ribonucleoprotein Complex Delivery: Methods and Applications for Neuroinflammation. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 14(4), 565-577. doi: 10.1007/s11481-019-09856-z. ID: 31172397.

14.) Bäck S, Dossat A, Parkkinen I, Koivula P, Airavaara M, Richie CT, Chen YH, Wang Y, Harvey BK (2019 Sep 13). Neuronal Activation Stimulates Cytomegalovirus Promoter-Driven Transgene Expression. Molecular therapy. Methods & clinical development, 14(), 180-188. doi: 10.1016/j.omtm.2019.06.006. ID: 31380464.

15.) Galvan A, Raper J, Hu X, Paré JF, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom PH, Oler JA, Kalin NH, Hall RA, Smith Y (2019 Sep). Ultrastructural localization of DREADDs in monkeys. The European journal of neuroscience, 50(5), 2801-2813. doi: 10.1111/ejn.14429. ID: 31063250.

16.) Nelson BS, Fulenwider HD, Nennig SE, Smith BM, Sequeira MK, Chimberoff SH, Richie CT, Cheng K, Rice KC, Harvey BK, Heilig M, Schank JR (2019 Aug 10). Escalated Alcohol Self-Administration and Sensitivity to Yohimbine-Induced Reinstatement in Alcohol Preferring Rats: Potential Role of Neurokinin-1 Receptors in the Amygdala. Neuroscience, 413(), 77-85. . ID: 31242442.

17.) Bäck S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK (2019 Apr 3). Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Neuron, 102(1), 105-119.e8. . ID: 30792150.

18.) Pardo-Garcia TR, Garcia-Keller C, Penaloza T, Richie CT, Pickel J, Hope BT, Harvey BK, Kalivas PW, Heinsbroek JA (2019 Mar 13). Ventral Pallidum Is the Primary Target for Accumbens D1 Projections Driving Cocaine Seeking. The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(11), 2041-2051. . ID: 30622165.

19.) Zallar LJ, Tunstall BJ, Richie CT, Zhang YJ, You ZB, Gardner EL, Heilig M, Pickel J, Koob GF, Vendruscolo LF, Harvey BK, Leggio L (2019 Feb). Development and initial characterization of a novel ghrelin receptor CRISPR/Cas9 knockout wistar rat model. International journal of obesity (2005), 43(2), 344-354. doi: 10.1038/s41366-018-0013-5. ID: 29453460.

20.) Campbell LA, Coke LM, Richie CT, Fortuno LV, Park AY, Harvey BK (2019 Jan 2). Gesicle-Mediated Delivery of CRISPR/Cas9 Ribonucleoprotein Complex for Inactivating the HIV Provirus. Molecular therapy : the journal of the American Society of Gene Therapy, 27(1), 151-163. . ID: 30389355.

21.) Trychta KA, Heathward EJ, Sulima A, Bäck S, Farokhnia M, Richie CT, Leggio L, Rice KC, Harvey BK (2018 Dec). Extracellular esterase activity as an indicator of endoplasmic reticulum calcium depletion. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 23(8), 756-765. doi: 10.1080/1354750X.2018.1490968. ID: 30095301.

22.) Li X, Carreria MB, Witonsky KR, Zeric T, Lofaro OM, Bossert JM, Zhang J, Surjono F, Richie CT, Harvey BK, Son H, Cowan CW, Nestler EJ, Shaham Y (2018 Aug 1). Role of Dorsal Striatum Histone Deacetylase 5 in Incubation of Methamphetamine Craving. Biological psychiatry, 84(3), 213-222. . ID: 29397902.

23.) Penttinen AM, Parkkinen I, Voutilainen MH, Koskela M, Bäck S, Their A, Richie CT, Domanskyi A, Harvey BK, Tuominen RK, Nevalaita L, Saarma M, Airavaara M (2018). Pre-α-pro-GDNF and Pre-β-pro-GDNF Isoforms Are Neuroprotective in the 6-hydroxydopamine Rat Model of Parkinson Frontiers in neurology, 9(), 457. doi: 10.3389/fneur.2018.00457. ID: 29973907.

24.) Campbell LA, Richie CT, Zhang Y, Heathward EJ, Coke LM, Park EY, Harvey BK (2017 Dec). In vitro modeling of HIV proviral activity in microglia. The FEBS journal, 284(23), 4096-4114. doi: 10.1111/febs.14293. ID: 29114997.

25.) Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez LA, Ellis RJ, Richie CT, Harvey BK, Dannals RF, Pomper MG, Bonci A, Michaelides M (2017 Aug 4). Chemogenetics revealed: DREADD occupancy and activation via converted clozapine. Science (New York, N.Y.), 357(6350), 503-507. . ID: 28774929.

26.) Hamra FK, Richie CT, Harvey BK (2017 Aug). Long Evans rat spermatogonial lines are effective germline vectors for transgenic rat production. Transgenic research, 26(4), 477-489. doi: 10.1007/s11248-017-0025-2. ID: 28608322.

27.) Sharpe MJ, Marchant NJ, Whitaker LR, Richie CT, Zhang YJ, Campbell EJ, Koivula PP, Necarsulmer JC, Mejias-Aponte C, Morales M, Pickel J, Smith JC, Niv Y, Shaham Y, Harvey BK, Schoenbaum G (2017 Jul 24). Lateral Hypothalamic GABAergic Neurons Encode Reward Predictions that Are Relayed to the Ventral Tegmental Area to Regulate Learning. Current biology : CB, 27(14), 2089-2100.e5. . ID: 28690111.

28.) Wright AM, Zapata A, Hoffman AF, Necarsulmer JC, Coke LM, Svarcbahs R, Richie CT, Pickel J, Hope BT, Harvey BK, Lupica CR (2021 Jul-Aug). Effects of Withdrawal from Cocaine Self-Administration on Rat Orbitofrontal Cortex Parvalbumin Neurons Expressing <i>Cre recombinase</i>: Sex-Dependent Changes in Neuronal Function and Unaltered Serotonin Signaling. eNeuro, 8(4), . . ID: 34083381.

Description:

Mission: The Genetic Engineering and Viral Vector Core (GEVVC) provides support to NIDA-IRP researchers by facilitating studies of brain function under physiological and pathological conditions through developing and producing genetic tools capable of modulating and monitoring the molecules, cells and circuits in the nervous system. Services: The NIDA GEVVC provides services in three main categories: 1) the custom design and/or production of viral vectors/nanoparticles, 2) in vitro molecular and cell biology services, 3) consultation and training.