Carleton University

Contact Info:

Leila Mostaço-Guidolin

1125 Colonel By Dr

Ottawa, ON K1S 5B6

https://teamhubottawa.com/

Grants and Identifiers:

RRID: RRID:SCR_022968

Instrumentation:

Services Provided:

3D Bioprinting

Biochemical Analysis

Bioimage Informatics

Cell Culture

Confocal Microscopy

Confocal/ Super Resolution Microscope

Consultation

Consultations

Data Analysis

Data Analysis And Interpretation

Data Processing

Data Processing And Interpretation

Data Visualization

Discovery Science

Fluorescence Microscope With Optical Sectioning Capability

Mass Spectrometry

Microscopy

Modeling And Simulation

Optical Imaging

Optical Spectroscopy

Outreach

Raman Spectroscopy

Sample Preparation

Super-resolution Microscopy

Tissue Culture

Western Blot

Relevant Publications:

1.) Poole JJA, Mostaço-Guidolin LB (2021 Jul 12). Optical Microscopy and the Extracellular Matrix Structure: A Review. Cells, 10(7), . . ID: 34359929.

2.) Allen CH, Ahmed D, Raiche-Tanner O, Chauhan V, Mostaço-Guidolin L, Cassol E, Murugkar S (2021 Jul 7). Label-free two-photon imaging of mitochondrial activity in murine macrophages stimulated with bacterial and viral ligands. Scientific reports, 11(1), 14081. doi: 10.1038/s41598-021-93043-9. ID: 34234166.

3.) Mostaço-Guidolin L, Rosin NL, Hackett TL (2017 Aug 15). Imaging Collagen in Scar Tissue: Developments in Second Harmonic Generation Microscopy for Biomedical Applications. International journal of molecular sciences, 18(8), . . ID: 28809791.

Description:

The TEAM Hub has four research facilities: 1. BioImaging Research Node houses our state-of-the-art commercial and custom imaging systems. Focused on optical imaging. 2. Tissue Engineering & Biomaterials Testing Research Node is equipped with a microfluidics 3D bioprinter and mechanical testers, which facilitate the development of artificial 3D biological tissue models and the biomechanical testing of different biomaterials and biomedical samples. 3. The Biochemistry and Molecular Biology Research Node houses our hypoxia chamber and systems for the characterization and quantification of proteins, peptides, and nucleic acids. 4. The Cellular Biology Research Node has been designed to support the development and use of 3D tissue models. It houses a flow cytometer for phenotyping and performing functional testing of cells and bacteria; a Seahorse Analyzer for evaluation of metabolic activity of cells; and an Incucyte for live-cell imaging of cells and tissues in culture.