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TEAM Hub - Tissue Engineering and Applied Materials Core Facility

Carleton University

Contact Info:

Leila Mostaço-Guidolin

1125 Colonel By Dr

Ottawa, ON K1S 5B6

https://teamhubottawa.com/

Grants and Identifiers:

RRID: RRID:SCR_022968

Instrumentation:

Sartorius IncuCyte S3 Live Cell Analysis System

System that automatically captures and analyzes images of living cells around-the-clock for days, weeks, or months, while cells remain undisturbed inside standard tissue culture incubator. Kinetic, image-based measurements. Used for cell monitoring and surveillance, cell health and viability, migration and invasion, phenotypic cell based assays. [Product Link]

RRID:SCR_023147

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Zeiss Axioplan 2 imaginig system

Upright microscopy imaging platform for complex research that is optimized for light transmission and extremely high contrast fluorescence imaging. This complete system comes fully configured for best performance, range and functionality. [Product Link]

RRID:SCR_020918

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ZEISS LSM 980 with Airyscan 2 Microscope

Upright laser scanning confocal microscope with Airyscan 2 technology. Airyscan module is new detector concept featuring 32 channel GaAsP-PMT area detector. Airyscan detector brings simultaneous improvements in spatial resolution and signal-to-noise ratio in comparison with conventional confocal microscopy. [Product Link]

RRID:SCR_025048

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Agilent Seahorse XFp Analyzer

Aspect Biosystems RX1 Microfluidics 3D Bioprinter

Baker Ruskinn InvivO2 400 Hypoxic Workstation

Custom Custom-built Multimodal NLO Microscope

Custom Custom-built Raman Microspectrometer

Sartorius Incucyte® S3 Live-Cell Analysis Instrument

Zeiss LSM 980 with Airyscan 2 Confocal Laser Scanning Microscope and NLOM module

Services Provided:

3D Bioprinting

Biochemical Analysis

Bioimage Informatics

Cell Culture

Confocal Microscopy

Confocal/ Super Resolution Microscope

Consultation

Consultations

Data Analysis

Data Analysis And Interpretation

Data Processing

Data Processing And Interpretation

Data Visualization

Discovery Science

Fluorescence Microscope With Optical Sectioning Capability

Mass Spectrometry

Microscopy

Modeling And Simulation

Optical Imaging

Optical Spectroscopy

Outreach

Raman Spectroscopy

Sample Preparation

Super-resolution Microscopy

Tissue Culture

Western Blot

Relevant Publications:

1.) Kaianathbhatta A, Al Daraawi M, Kunchur NN, Mejlaoui R, Versey Z, Cassol E, Mostaço-Guidolin LB (2026 Jan 24). MitoTex (Mitochondria Texture Analysis User Interface): Open-Source Framework for Textural Characterization and Classification of Mitochondrial Structures. International journal of molecular sciences, 27(3), . . PMCID: 12897202.


2.) Poole JJA, Mostaço-Guidolin LB (2021 Jul 12). Optical Microscopy and the Extracellular Matrix Structure: A Review. Cells, 10(7), . . ID: 34359929.


3.) Allen CH, Ahmed D, Raiche-Tanner O, Chauhan V, Mostaço-Guidolin L, Cassol E, Murugkar S (2021 Jul 7). Label-free two-photon imaging of mitochondrial activity in murine macrophages stimulated with bacterial and viral ligands. Scientific reports, 11(1), 14081. doi: 10.1038/s41598-021-93043-9. ID: 34234166.


4.) Mostaço-Guidolin L, Rosin NL, Hackett TL (2017 Aug 15). Imaging Collagen in Scar Tissue: Developments in Second Harmonic Generation Microscopy for Biomedical Applications. International journal of molecular sciences, 18(8), . . ID: 28809791.


Description:

The TEAM Hub has four research facilities: 1. BioImaging Research Node houses our state-of-the-art commercial and custom imaging systems. Focused on optical imaging. 2. Tissue Engineering & Biomaterials Testing Research Node is equipped with a microfluidics 3D bioprinter and mechanical testers, which facilitate the development of artificial 3D biological tissue models and the biomechanical testing of different biomaterials and biomedical samples. 3. The Biochemistry and Molecular Biology Research Node houses our hypoxia chamber and systems for the characterization and quantification of proteins, peptides, and nucleic acids. 4. The Cellular Biology Research Node has been designed to support the development and use of 3D tissue models. It houses a flow cytometer for phenotyping and performing functional testing of cells and bacteria; a Seahorse Analyzer for evaluation of metabolic activity of cells; and an Incucyte for live-cell imaging of cells and tissues in culture.