Sign In

Search |

Add/Edit My Facility

UTA CBRS Sauer Structural Biology Laboratory

The University of Texas at Austin

Contact Info:

Axel Brilot

102 E 24th street

FNT B.107

Austin, TX 78712

https://research.utexas.edu/cbrs/cores/cem/

Grants and Identifiers:

RRID: RRID:SCR_022951

Instrumentation:

FEI Aquilos 2 Cryo-FIB for Life Sciences

Sample preparation tool used for the preparation of thin, electron transparent lamellas for high resolution cryo electron tomography or MicroED of micro crystals. [Product Link]

RRID:SCR_019880

cite this instrument

FEI Glacios Cryo-TEM (Transmission Electron Microscope) for Life Sciences

Microscope that features 200 kV XFEG optics and ensures a robust and contamination-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing. [Product Link]

RRID:SCR_019883

cite this instrument

Gatan BioContinuum Imaging Filter Transmission Electron Microscope (TEM) Imaging and Spectroscopy

Microscope imaging filter that is optimized for cryo-EM and cryo-ET to gain further insight into system function and disease progression at the molecular level. [Product Link]

RRID:SCR_019924

cite this instrument

Gatan K3 Camera Transmission Electron Microscope (TEM) Imaging and Spectroscopy

Camera that is optimized for low-dose electron microscopy (EM) applications in both life science and materials science research and captures 1,500 full frames per second. [Product Link]

RRID:SCR_019937

cite this instrument

Jeol JEOL JEM-1400Flash Transmission Electron Microscope

Transmission electron microscope that can be used to first view samples at low magnification before studying fine structures of interest at high magnification. [Product Link]

RRID:SCR_020179

cite this instrument

FEI FEI Titan Krios G3

300kV cryo-capable transmission electron microscope equipped with 12-grid autoloader.

(Equipment No Longer Active At this Facility)

Thermo-Fisher Scientific Thermo-Fisher Scientific Falcon IV direct electron detector

Fourth generation direct electron detector from Thermo-Fisher scientific. The camera is capable of achieving electon counting at a frame rate of 250fps and saving electron counting events in eer format.

Services Provided:

Biological photography/Photomicrography

Consultation

Cryo-Electron Microscopy

Electron Microscopy

Microscopy

Sample Preparation

Structural Biology

Structure Determination

Training

Transmission Electron Microscopy

Relevant Publications:

1.) Malady BT, Papagiannoula A, Kamatar A, Sarkar S, Lebrun GT, Wang L, Hayden CC, Lafer EM, Owen DJ, Milles S, Stachowiak JC (2025 Oct 15). Of condensates and coats - reciprocal regulation of clathrin assembly and the growth of protein networks Nature Communications, 16(), 9139. . PMCID: 12528380.


2.) von Jeinsen NA, Ward DJ, Bergin M, Lambrick SM, Williamson DM, Langford RM, Dawson LF, Rana V, Shivaswamy S, Zhou X, Mikesh M, Gordon VD, Wren BW, Brown KA, Dastoor PC (2025 Oct 3). Surface visualisation of bacterial biofilms using neutral atom microscopy Journal of Microscopy, 301(1), 107-115. . PMCID: 12746369.


3.) Nayak A, Nayak D, Jia L, Ruben EA, Viswanadhapalli S, dos Santos Bury P, Nassar KM, Yu CH, Tumanova AA, Stratton CM, Ebadi P, Ivanov DN, Sung P, Vadlamudi RK, Wasmuth EV, Olsen SK (2025 Sep 25). Cryo-EM structures reveal the molecular mechanism of SUMO E1–E2 thioester transfer Nature Structural & Molecular Biology, 32(12), 2441-2453. . PMCID: 12700794.


4.) McCafferty CL, Hoogerbrugge G, Papoulas O, Schwartz EA, Ritchey S, Taylor DW, Brilot AF, Marcotte EM (2025 Sep 22). The CAGE complex: a hollow, megadalton, protein assembly in prokaryotic and eukaryotic microbes bioRxiv, (), 2025.09.22.677704. . PMCID: 12485934.


5.) Heiser BJ, Lewis MM, Mohammadi Zerankeshi M, Netemeyer EK, Hernandez AM, Marras AE, Ghosh D (2025 Jul 18). Systematic screening of excipients to stabilize aerosolized lipid nanoparticles for enhanced mRNA delivery Rsc Pharmaceutics, (), . . PMCID: 12272335.


6.) Musalgaonkar S, Yelland JN, Chitale R, Rao S, Ozadam H, Taylor DW, Cenik C, Johnson AW (2025 Feb 3). The assembly factor Reh1 is released from the ribosome during its initial round of translation Nature Communications, 16(), 1278. . PMCID: 11791190.


7.) Park J, Bartzoka F, von Beck T, Li ZN, Mishina M, Hebert LS, Kain J, Liu F, Sharma S, Cao W, Eddins DJ, Kumar A, Kim JE, Lee JS, Wang Y, Schwartz EA, Brilot AF, Satterwhite E, Towers DM, McKnight E, Pohl J, Thompson MG, Gaglani M, Dawood FS, Naleway AL, Stevens J, Kennedy RB, Jacob J, Lavinder JJ, Levine MZ, Gangappa S, Ippolito GC, Sambhara S, Georgiou G (2025 Feb). Molecular features of the serological IgG repertoire elicited by egg-based, cell-based, or recombinant haemagglutinin-based seasonal influenza vaccines: a comparative, prospective, observational cohort study. The Lancet. Microbe, 6(2), 100935. . PMID: 39667375.


8.) Musalgaonkar S, Yelland J, Chitale R, Rao S, Ozadam H, Cenik C, Taylor D, Johnson A (2023 Oct 23). The Ribosome Assembly Factor Reh1 is Released from the Polypeptide Exit Tunnel in the Pioneer Round of Translation. bioRxiv : the preprint server for biology, (), . . PMID: 37961559.


9.) Afsar M, Liu G, Jia L, Ruben EA, Nayak D, Sayyad Z, Bury PD, Cano KE, Nayak A, Zhao XR, Shukla A, Sung P, Wasmuth EV, Gack MU, Olsen SK (2023 Aug 8). Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer Nature Communications, 14(), 4786. . PMCID: 10409785.


10.) Rawal Y, Jia L, Meir A, Zhou S, Kaur H, Ruben EA, Kwon Y, Bernstein KA, Jasin M, Taylor AB, Burma S, Hromas R, Mazin AV, Zhao W, Zhou D, Wasmuth EV, Greene EC, Sung P, Olsen SK (2023 Jul). Structural insights into BCDX2 complex function in homologous recombination. Nature, 619(7970), 640-649. . PMID: 37344589.


11.) Jara MO, Warnken ZN, Sahakijpijarn S, Thakkar R, Kulkarni VR, Christensen DJ, Koleng JJ, Williams RO III (2022 Nov 23). Oral Delivery of Niclosamide as an Amorphous Solid Dispersion That Generates Amorphous Nanoparticles during Dissolution Pharmaceutics, 14(12), 2568. . PMCID: 9785291.


Description:

The Sauer Structural Biology Laboratory (SSBL) is a state-of-the-art cryo-EM facility. The SSBL offers assisted and unassisted use of our high-end TEMs and ancillary sample preparation equipment, training, and collaborations.