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UTA CBRS Sauer Structural Biology Laboratory

The University of Texas at Austin

Contact Info:

Axel Brilot

102 E 24th street

FNT B.107

Austin, TX 78712

https://research.utexas.edu/cbrs/cores/cem/

Grants and Identifiers:

RRID: RRID:SCR_022951

Instrumentation:

FEI Glacios Cryo-TEM (Transmission Electron Microscope) for Life Sciences

Microscope that features 200 kV XFEG optics and ensures a robust and contamination-free pathway throughout the entire workflow, from sample preparation and optimization to image acquisition and data processing. [Product Link]

RRID:SCR_019883

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Gatan BioContinuum Imaging Filter Transmission Electron Microscope (TEM) Imaging and Spectroscopy

Microscope imaging filter that is optimized for cryo-EM and cryo-ET to gain further insight into system function and disease progression at the molecular level. [Product Link]

RRID:SCR_019924

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Gatan K3 Camera Transmission Electron Microscope (TEM) Imaging and Spectroscopy

Camera that is optimized for low-dose electron microscopy (EM) applications in both life science and materials science research and captures 1,500 full frames per second. [Product Link]

RRID:SCR_019937

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FEI FEI Titan Krios G3

300kV cryo-capable transmission electron microscope equipped with 12-grid autoloader.

Thermo-Fisher Scientific Thermo-Fisher Scientific Falcon IV direct electron detector

Fourth generation direct electron detector from Thermo-Fisher scientific. The camera is capable of achieving electon counting at a frame rate of 250fps and saving electron counting events in eer format.

Services Provided:

Biological photography/Photomicrography

Consultation

Cryo-Electron Microscopy

Electron Microscopy

Microscopy

Sample Preparation

Structural Biology

Structure Determination

Training

Transmission Electron Microscopy

Relevant Publications:

1.) Musalgaonkar S, Yelland J, Chitale R, Rao S, Ozadam H, Cenik C, Taylor D, Johnson A (2023 Oct 23). The Ribosome Assembly Factor Reh1 is Released from the Polypeptide Exit Tunnel in the Pioneer Round of Translation. bioRxiv : the preprint server for biology, (), . . PMID: 37961559.


2.) Afsar M, Liu G, Jia L, Ruben EA, Nayak D, Sayyad Z, Bury PD, Cano KE, Nayak A, Zhao XR, Shukla A, Sung P, Wasmuth EV, Gack MU, Olsen SK (2023 Aug 8). Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer Nature Communications, 14(), 4786. . PMCID: 10409785.


3.) Afsar M, Liu G, Jia L, Ruben EA, Nayak D, Sayyad Z, Bury PDS, Cano KE, Nayak A, Zhao XR, Shukla A, Sung P, Wasmuth EV, Gack MU, Olsen SK (2023 Aug 8). Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer. Nature communications, 14(1), 4786. . PMID: 37553340.


4.) Rawal Y, Jia L, Meir A, Zhou S, Kaur H, Ruben EA, Kwon Y, Bernstein KA, Jasin M, Taylor AB, Burma S, Hromas R, Mazin AV, Zhao W, Zhou D, Wasmuth EV, Greene EC, Sung P, Olsen SK (2023 Jul). Structural insights into BCDX2 complex function in homologous recombination. Nature, 619(7970), 640-649. . PMID: 37344589.


5.) Jara MO, Warnken ZN, Sahakijpijarn S, Thakkar R, Kulkarni VR, Christensen DJ, Koleng JJ, Williams RO III (2022 Nov 23). Oral Delivery of Niclosamide as an Amorphous Solid Dispersion That Generates Amorphous Nanoparticles during Dissolution Pharmaceutics, 14(12), 2568. . PMCID: 9785291.


6.) Jara MO, Warnken ZN, Sahakijpijarn S, Thakkar R, Kulkarni VR, Christensen DJ, Koleng JJ, Williams RO 3rd (2022 Nov 23). Oral Delivery of Niclosamide as an Amorphous Solid Dispersion That Generates Amorphous Nanoparticles during Dissolution. Pharmaceutics, 14(12), . . PMID: 36559062.


Description:

The Sauer Structural Biology Laboratory (SSBL) is a state-of-the-art cryo-EM facility. The SSBL offers assisted and unassisted use of our high-end TEMs and ancillary sample preparation equipment, training, and collaborations.