Harvard Medical School
Contact Info:
Michael Peluso
77 Avenue Louis Pasteur
NRB 837
Boston, MA 02115
https://www.dfhcc.harvard.edu/research/core-facilities/mouse-engineering/
Grants and Identifiers:
RRID: RRID:SCR_022950
Instrumentation:
Services Provided:
Embryo Cryopreservation & Recovery Services
Relevant Publications:
Description:
The Mouse Engineering Core was established in 1992 operating at the BWH, and is now located at Harvard Medical School. The Mouse Engineering Core provides services for the generation of transgenic and knockout mice using state-of-the-art facilities and equipment. The Core has all of the equipment necessary for generation of transgenic mice including a Nikon Diaphot microscope equipped with Nomarski Optics and Narishige micromanipulators for microinjections, Nikon surgical microscopes for egg isolation and transfer, a sutter needle puller and a de Fonbrune microforge. The facility maintains mice necessary for egg donors, egg recipients, and vasectomized males. ES cell culture services have been offered for 15 years. The core offers electroporation of targeting vectors into ES cells and provides investigators with DNA to identify ES cells carrying the desired recombination events; and ES cell expansion for microinjection into blastocysts. The core\'s newest services include CRISPR injections, sperm and embryo cryopreservation, and reanimation with fresh or frozen sperm via IVF or the thawing and implanting embryos.
