University of Ottawa

Contact Info:

James Taylor

451 Smyth Rd

Ottawa, ON K1H 8M5

https://www2.uottawa.ca/faculty-medicine/core-facilities/gem

Grants and Identifiers:

RRID: RRID:SCR_022954

Instrumentation:

Services Provided:

Cloning

Consultation

CRISPR

CRISPR/Cas Project Design

DNA Analysis

Relevant Publications:

1.) Akuma M, Kim M, Zhu C, Wiljer E, Gaudreau-Lapierre A, Patterson LD, Egevad L, Tanguay S, Trinkle-Mulcahy L, Stanford WL, Riazalhosseini Y, Russell RC (2025 Apr 16). Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinoma Cell Death & Disease, 16(1), 307. . PMCID: 12003641.

2.) Suk TR, Part CE, Zhang JL, Nguyen TT, Heer MM, Caballero-Gómez A, Grybas VS, McKeever PM, Nguyen B, Ali T, Callaghan SM, Woulfe JM, Robertson J, Rousseaux MW (2025 Mar 28). A stress-dependent TDP-43 SUMOylation program preserves neuronal function Molecular Neurodegeneration, 20(), 38. . PMCID: 11951803.

3.) Minarrieta L, Annis MG, Audet-Delage Y, Kuasne H, Pacis A, St-Louis C, Nowakowski A, Biondini M, Khacho M, Park M, Siegel PM, St-Pierre J (2024 Nov 6). Mitochondrial elongation impairs breast cancer metastasis Science Advances, 10(45), eadm8212. . PMCID: 11540020.

4.) Dias AP, Rehmani T, Applin BD, Salih M, Tuana B (2024 Oct). SLMAP3 is crucial for organogenesis through mechanisms involving primary cilia formation. Open biology, 14(10), rsob240206. . PMID: 39417621.

Description:

A facility assisting researchers in mammalian genome editing and molecular biology, including CRISPR genetic libraries and screens as well as custom viral vector/particle production. Projects are customized to the researchers needs, from full-service cell line creation to design and cloning.