University of Chicago
Hardik Shah
900 E 57th St, Room 4142
Chicago, IL 60637
https://voices.uchicago.edu/metabolomics/
RRID: RRID:SCR_022932
Thermo Scientific Orbitrap ID-X Tribrid mass spectrometers
Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometers
Lipid Profiling (Lipidomics)
Polar Metabolite Profiling
Quantitative Targeted Analysis For Selected Metabolites
Stable Isotope Tracing
1.) Zhang FQ, Li J, Zhang R, Tu J, Xie Z, Tsuji T, Shah H, Ross MO, Lyu R, Matsuzaki J, Tabor A, Xue K, Choudhry F, Yin C, Youshanlouei HR, Shah S, Drazer MW, He YY, Bissonnette BM, Li Y, Mao H, Huang J, Dong L, Su R, He C, Odunsi K, Chen J, Fan H (2025 Sep 16). Zeaxanthin augments CD8(+) effector T cell function and immunotherapy efficacy. Cell reports. Medicine, 6(9), 102324. doi: 10.1016/j.xcrm.2025.102324. ID: 40897177.
2.) Liu R, Zhang Z, Kyaw AK, Grabińska KA, Shah H, Shen H (2025 Sep 9). Cellular pan-chain acyl-CoA profiling reveals SLC25A42/SLC25A16 in mitochondrial CoA import and metabolism. Nature metabolism, (), . doi: 10.1038/s42255-025-01358-y. ID: 40925986.
3.) Li S, Shapiro JW, Shah H, Higgs EF, Xie L, Li Y, Zha Y, Trujillo J, Cabanov A, Jones TA, Flood B, Hatogai K, Tonea R, Kline J, Gajewski TF (2025 Aug 1). Tumor Cell-Intrinsic Decr2 Regulates Ferroptosis and Immunotherapy Efficacy. Cancer immunology research, 13(8), 1284-1302. . ID: 40512146.
4.) Tiwari V, Jin B, Sun O, Lopez Gonzalez EDJ, Chen MH, Wu X, Shah H, Zhang A, Herman MA, Spracklen CN, Goodman RP, Brenner C (2025 Jun 25). Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in Citrin Deficiency. bioRxiv : the preprint server for biology, (), . . ID: 39763913.
5.) Hamanaka RB, Shin KWD, Atalay MV, Cetin-Atalay R, Shah H, Houpy Szafran JC, Woods PS, Meliton AY, Shamaa OR, Tian Y, Cho T, Mutlu GM (2025 Jun 17). Arginine promotes the activation of human lung fibroblasts independent of its metabolism. The Biochemical journal, 482(12), 823-38. doi: 10.1042/BCJ20253033. ID: 40454944.
6.) Pan X, Munan S, Zuckerman AL, Pon A, Violante S, Cross JR, Shah H, Cracan V (2025 Jun 5). A genetically encoded bifunctional enzyme mitigates redox imbalance and lipotoxicity via engineered Gro3P-Glycerol shunt. bioRxiv : the preprint server for biology, (), . . ID: 40501877.
7.) Wang Y, Wilfahrt D, Jonker P, Lontos K, Cai C, Cameron B, Xie B, Peralta RM, Schoedel ER, Gunn WG, AminiTabrizi R, Shah H, Rivadeneira DB, Muir A, Delgoffe GM (2025 May). Tumour interstitial fluid-enriched phosphoethanolamine suppresses T cell function. Nature cell biology, 27(5), 835-846. doi: 10.1038/s41556-025-01650-9. ID: 40258951.
8.) Jonker PB, Sadullozoda M, Cognet G, Saab JJ, Sokol KH, Wu VX, Kumari D, Sheehan C, Ozgurses ME, Agovino D, Croley G, Patel SA, Bock-Hughes A, Macleod KF, Shah H, Coloff JL, Lien EC, Muir A (2025 Mar 13). Microenvironmental arginine restriction sensitizes pancreatic cancers to polyunsaturated fatty acids by suppression of lipid synthesis bioRxiv, (), 2025.03.10.642426. . PMCID: 11952453.
9.) Wang B, Fu W, Ueda A, Shah H, Wu CF, Chi W, Zhuang X (2025 Mar 7). Genetic vitamin B6 deficiency and alcohol interaction in behavior and metabolism. bioRxiv : the preprint server for biology, (), . . ID: 40093095.
10.) Nengroo MA, Klein AT, Carr HS, Vidal-Cruchez O, Sahu U, McGrail DJ, Sahni N, Gao P, Asara JM, Shah H, Mendillo ML, Ben-Sahra I (2025 Mar 1). Succinate dehydrogenase activity supports de novo purine synthesis. bioRxiv : the preprint server for biology, (), . . ID: 40060604.
11.) Liu R, Ren X, Park YE, Feng H, Sheng X, Song X, AminiTabrizi R, Shah H, Li L, Zhang Y, Abdullah KG, Dubois-Coyne S, Lin H, Cole PA, DeBerardinis RJ, McBrayer SK, Huang H, Zhao Y (2025 Feb 4). Nuclear GTPSCS functions as a lactyl-CoA synthetase to promote histone lactylation and gliomagenesis. Cell metabolism, 37(2), 377-394.e9. . ID: 39642882.
12.) Hamanaka RB, Shin KWD, Atalay MV, Cetin-Atalay R, Shah H, Szafran JCH, Woods PS, Meliton AY, Shamaa OR, Tian Y, Cho T, Mutlu GM (2024 Nov 1). Role of Arginine and its Metabolism in TGF-β-Induced Activation of Lung Fibroblasts. bioRxiv : the preprint server for biology, (), . . ID: 39554075.
13.) Hecht F, Zocchi M, Tuttle ET, Ward NP, Smith B, Kang YP, Cazarin J, Soares ZG, Ozgurses ME, Zhao H, Sheehan C, Alimohammadi F, Munger LD, Trivedi D, Asantewaa G, Blick-Nitko SK, Zoeller JJ, Chen Y, Vasiliou V, Turner BM, Muir A, Coloff JL, Munger J, DeNicola GM, Harris IS (2024 Oct 13). Catabolism of extracellular glutathione supplies amino acids to support tumor growth. bioRxiv : the preprint server for biology, (), . . PMID: 39416022.
14.) Yang H, Zingaro VA, Lincoff J, Tom H, Oikawa S, Oses-Prieto JA, Edmondson Q, Seiple I, Shah H, Kajimura S, Burlingame AL, Grabe M, Ruggero D (2024 Sep). Remodelling of the translatome controls diet and its impact on tumorigenesis. Nature, 633(8028), 189-197. doi: 10.1038/s41586-024-07781-7. PMID: 39143206.
15.) Song X, Tiek D, Miki S, Huang T, Lu M, Goenka A, Iglesia R, Yu X, Wu R, Walker M, Zeng C, Shah H, Weng SHS, Huff A, Zhang W, Koga T, Hubert C, Horbinski CM, Furnari FB, Hu B, Cheng SY (2024 Apr 25). RNA splicing analysis deciphers developmental hierarchies and reveals therapeutic targets in adult glioma. The Journal of clinical investigation, 134(11), . . PMID: 38662454.
16.) Singh C, Jin B, Shrestha N, Markhard AL, Panda A, Calvo SE, Deik A, Pan X, Zuckerman AL, Ben Saad A, Corey KE, Sjoquist J, Osganian S, AminiTabrizi R, Rhee EP, Shah H, Goldberger O, Mullen AC, Cracan V, Clish CB, Mootha VK, Goodman RP (2024 Jan 2). ChREBP is activated by reductive stress and mediates GCKR-associated metabolic traits. Cell metabolism, 36(1), 144-158.e7. . PMID: 38101397.
The platform is equipped with 3 of the high-resolution Thermo Scientific Orbitrap Tribrid mass spectrometers( 2 IQX +1-IDX) coupled to – Vanquish Horizon UHPLC systems and dedicated to (1) full-scan metabolomics for polar metabolite profiling using HILIC chromatography and (2) full-scan non-polar lipid profiling using reverse phase chromatography. The platform has established in-house retention time and MS/MS databases of ~900 reference standards and actively sourcing depending on the sample types. We have experience in quantifying diverse groups of metabolites in a wide variety of matrices such as cells, culture medium, bodily fluid, animal tissue, exhaled breath condensate, etc., and performing stable isotope tracing experiments using 13C, 15N, and 2H-labeled substrates. We support hypothesis-generating untargeted and hypothesis-driven targeted metabolomics/lipidomics studies from the experimental design stage through data acquisition, data analysis, and interpretation.
