University of Chicago
Hardik Shah
900 E 57th St, Room 4142
Chicago, IL 60637
https://voices.uchicago.edu/metabolomics/
RRID: RRID:SCR_022932
Metabolomics Platform
1.) Jonker PB, Sadullozoda M, Cognet G, Saab JJ, Sokol KH, Wu VX, Kumari D, Sheehan C, Ozgurses ME, Agovino D, Croley G, Patel SA, Bock-Hughes A, Macleod KF, Shah H, Coloff JL, Lien EC, Muir A (2025 Mar 13). Microenvironmental arginine restriction sensitizes pancreatic cancers to polyunsaturated fatty acids by suppression of lipid synthesis bioRxiv, (), 2025.03.10.642426. . PMCID: 11952453.
2.) Hecht F, Zocchi M, Tuttle ET, Ward NP, Smith B, Kang YP, Cazarin J, Soares ZG, Ozgurses ME, Zhao H, Sheehan C, Alimohammadi F, Munger LD, Trivedi D, Asantewaa G, Blick-Nitko SK, Zoeller JJ, Chen Y, Vasiliou V, Turner BM, Muir A, Coloff JL, Munger J, DeNicola GM, Harris IS (2024 Oct 13). Catabolism of extracellular glutathione supplies amino acids to support tumor growth. bioRxiv : the preprint server for biology, (), . . PMID: 39416022.
3.) Yang H, Zingaro VA, Lincoff J, Tom H, Oikawa S, Oses-Prieto JA, Edmondson Q, Seiple I, Shah H, Kajimura S, Burlingame AL, Grabe M, Ruggero D (2024 Sep). Remodelling of the translatome controls diet and its impact on tumorigenesis. Nature, 633(8028), 189-197. doi: 10.1038/s41586-024-07781-7. PMID: 39143206.
4.) Song X, Tiek D, Miki S, Huang T, Lu M, Goenka A, Iglesia R, Yu X, Wu R, Walker M, Zeng C, Shah H, Weng SHS, Huff A, Zhang W, Koga T, Hubert C, Horbinski CM, Furnari FB, Hu B, Cheng SY (2024 Apr 25). RNA splicing analysis deciphers developmental hierarchies and reveals therapeutic targets in adult glioma. The Journal of clinical investigation, 134(11), . . PMID: 38662454.
5.) Singh C, Jin B, Shrestha N, Markhard AL, Panda A, Calvo SE, Deik A, Pan X, Zuckerman AL, Ben Saad A, Corey KE, Sjoquist J, Osganian S, AminiTabrizi R, Rhee EP, Shah H, Goldberger O, Mullen AC, Cracan V, Clish CB, Mootha VK, Goodman RP (2024 Jan 2). ChREBP is activated by reductive stress and mediates GCKR-associated metabolic traits. Cell metabolism, 36(1), 144-158.e7. . PMID: 38101397.
The platform is equipped with the high-resolution Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer(+UVPD)- Vanquish Horizon UHPLC system. We have experience in quantifying diverse groups of metabolites in a wide variety of matrices such as cells, culture medium, bodily fluid, animal tissue, exhaled breath condensate, etc., and performing stable isotope tracing experiments using 13C, 15N, and 2H-labeled substrates. We support hypothesis-generating untargeted and hypothesis-driven targeted metabolomics studies from the experimental design stage through data acquisition, data analysis, and interpretation.
