University of Chicago
Samuel Weng
900 E 57th St
Room 4140
Chicago, IL 60637
https://voices.uchicago.edu/proteomics/
RRID: RRID:SCR_022928
Thermo Scientific Dionex Ultimate 3000 system
UltiMate 3000 Nano LC is a Liquid Chromatography Mass Spectrometer system that has been designed to optimize low flow separations and facilitate easy coupling to mass spectrometry to provide the best resolution, sensitivity, and selectivity for nano LC and proteomics applications. Dionex has played a pioneering role in the field of nano HPLC, a technique that generally involves the application of columns with an internal diameter of 75,?m and low flow rates of around 300 nL/min. These configurations are best for analysis with limited sample starting amounts, due to the sensitivity enhancement of the smaller column i.d. Dionex offers several solutions to accommodate and robust nano LC as a front end to mass spectrometry and to fulfill three typical requirements: [Product Link]
RRID:SCR_020563
Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer
Thermo Scientific Orbitrap Exploris 480 Quadrupole Mass Spectrometer
Proteomics
1.) Mousaei Ghasroldasht M, Liakath Ali F, Park HS, Hadizadeh M, Weng SH, Huff A, Vafaei S, Al-Hendy A (2024 Apr 30). A Comparative Analysis of Naïve Exosomes and Enhanced Exosomes with a Focus on the Treatment Potential in Ovarian Disorders Journal of Personalized Medicine, 14(5), 482. . PMCID: 11122298.
2.) Song X, Tiek D, Miki S, Huang T, Lu M, Goenka A, Iglesia R, Yu X, Wu R, Walker M, Zeng C, Shah H, Weng SHS, Huff A, Zhang W, Koga T, Hubert C, Horbinski CM, Furnari FB, Hu B, Cheng SY (2024 Apr 25). RNA splicing analysis deciphers developmental hierarchies and reveals therapeutic targets in adult glioma. The Journal of clinical investigation, 134(11), . . PMID: 38662454.
3.) Hou Y, Zhang W, McGilvray PT, Sobczyk M, Wang T, Weng SHS, Huff A, Huang S, Pena N, Katanski CD, Pan T (2024 Feb 7). Engineered mischarged transfer RNAs for correcting pathogenic missense mutations. Molecular therapy : the journal of the American Society of Gene Therapy, 32(2), 352-371. . PMID: 38104240.
4.) Chaerkady R, Zhou Y, Delmar JA, Weng SHS, Wang J, Awasthi S, Sims D, Bowen MA, Yu W, Cazares LH, Sims GP, Hess S (2021 Jun 4). Characterization of Citrullination Sites in Neutrophils and Mast Cells Activated by Ionomycin via Integration of Mass Spectrometry and Machine Learning. Journal of proteome research, 20(6), 3150-3164. doi: 10.1021/acs.jproteome.1c00028. ID: 34008986.
5.) Uzozie AC, Ergin EK, Rolf N, Tsui J, Lorentzian A, Weng SSH, Nierves L, Smith TG, Lim CJ, Maxwell CA, Reid GSD, Lange PF (2021 Mar 15). PDX models reflect the proteome landscape of pediatric acute lymphoblastic leukemia but divert in select pathways. Journal of experimental & clinical cancer research : CR, 40(1), 96. doi: 10.1186/s13046-021-01835-8. ID: 33722259.
6.) Cazares LH, Chaerkady R, Samuel Weng SH, Boo CC, Cimbro R, Hsu HE, Rajan S, Dall (2020 Oct 20). Development of a Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of SARS-CoV-2 Spike Glycoprotein and Nucleoprotein. Analytical chemistry, 92(20), 13813-13821. doi: 10.1021/acs.analchem.0c02288. ID: 32966064.
7.) Weng SSH, Demir F, Ergin EK, Dirnberger S, Uzozie A, Tuscher D, Nierves L, Tsui J, Huesgen PF, Lange PF (2019 Nov). Sensitive Determination of Proteolytic Proteoforms in Limited Microscale Proteome Samples. Molecular & cellular proteomics : MCP, 18(11), 2335-2347. . ID: 31471496.
8.) Weng S, Li X, Li Y, Yu HZ (2016 Nov 21). Optical disc technology-enabled analytical devices: from hardware modification to digitized molecular detection. The Analyst, 141(22), 6190-6201. . ID: 27704085.
9.) Weng S, Li X, Niu M, Ge B, Yu HZ (2016 Jul 5). Blu-ray Technology-Based Quantitative Assays for Cardiac Markers: From Disc Activation to Multiplex Detection. Analytical chemistry, 88(13), 6889-96. doi: 10.1021/acs.analchem.6b01604. ID: 27268387.
10.) Fiel SA, Yang H, Schaffer P, Weng S, Inkster JA, Wong MC, Li PC (2015 Jun 17). Magnetic Droplet Microfluidics as a Platform for the Concentration of [18F]Fluoride and Radiosynthesis of Sulfonyl [18F]Fluoride. ACS applied materials & interfaces, 7(23), 12923-9. doi: 10.1021/acsami.5b02631. ID: 26000709.
11.) Li X, Weng S, Ge B, Yao Z, Yu HZ (2014 May 21). DVD technology-based molecular diagnosis platform: quantitative pregnancy test on a disc. Lab on a chip, 14(10), 1686-94. doi: 10.1039/c3lc51411k. ID: 24695902.
12.) Chakrabarti L, Chaerkady R, Wang J, Weng SHS, Wang C, Qian C, Cazares L, Hess S, Amaya P, Zhu J, Hatton D (2022 Jan-Dec). Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability. mAbs, 14(1), 2020081. doi: 10.1080/19420862.2021.2020081. ID: 35030984.
The mission of the facility is to work in collaboration with groups from UChicago and external organizations or companies to develop and utilize top-notch proteomics techniques to answer both basic and translational scientific questions.
