Van Andel Institute

Contact Info:

Tristan Kempston

333 Bostwick ave NE

Grand Rapids,, MI 49503

https://vivariumandtransgenicscore.vai.org/

Grants and Identifiers:

RRID: RRID:SCR_022914

Instrumentation:

Services Provided:

CRISPR

CRISPR Rosa26 & CRISPR H11

CRISPR/Cas Project Design

DNA Isolation

Embryo Cryopreservation & Recovery Services

Genome Engineering

Genotyping

Rederivation

Rederivation (Mouse)

Sperm Cryopreservation

Surgical Services

Transgenics

Relevant Publications:

1.) Panzeri I, Fagnocchi L, Apostle S, Tompkins M, Wolfrum E, Madaj Z, Hostetter G, Liu Y, Schaefer K, Yang CH, Bergsma A, Drougard A, Dror E, Chandler DP, Schramek D, Triche TJ Jr, Pospisilik JA (2025 Jan 24). TRIM28-dependent developmental heterogeneity determines cancer susceptibility through distinct epigenetic states Nature Cancer, 6(2), 385-403. . PMCID: 11864977.

2.) Panzeri I, Madaj Z, Fagnocchi L, Apostle S, Tompkins M, Hostetter G, Pospisilik JA (2024 Oct 16). Chronic obesity does not alter cancer survival in Tp53R270H/+ mice bioRxiv, (), 2024.10.14.618190. . PMCID: 11507782.

3.) Panzeri I, Madaj Z, Fagnocchi L, Apostle S, Tompkins M, Hostetter G, Pospisilik JA (2024 Oct 16). Chronic obesity does not alter cancer survival in Tp53 (R270H/+) mice. bioRxiv : the preprint server for biology, (), . . PMID: 39463991.

4.) Panzeri I, Fagnocchi L, Apostle S, Tompkins M, Wolfrum E, Madaj Z, Hostetter G, Liu Y, Schaefer K, Chih-Hsiang Y, Bergsma A, Drougard A, Dror E, Chandler D, Schramek D, Triche TJ Jr, Pospisilik JA (2023 Sep 15). Developmental priming of cancer susceptibility bioRxiv, (), 2023.09.12.557446. . PMCID: 10515831.

5.) Diegel CR, Kramer I, Moes C, Foxa GE, McDonald MJ, Madaj ZB, Guth S, Liu J, Harris JL, Kneissel M, Williams BO (2023 Aug 24). Inhibiting WNT secretion reduces high bone mass caused by Sost loss-of-function or gain-of-function mutations in Lrp5. Bone research, 11(1), 47. . PMID: 37612291.

6.) Waldhart AN, Lau KH, Dykstra H, Avequin T, Wu N (2023 May 19). Optimal HSF1 activation in response to acute cold stress in BAT requires nuclear TXNIP. iScience, 26(5), 106538. . PMID: 37168572.

7.) Liegel RP, Michalski MN, Vaidya S, Bittermann E, Finnerty E, Menke CA, Diegel CR, Zhong ZA, Williams BO, Stottmann RW (2023 Feb 15). Successful therapeutic intervention in new mouse models of frizzled 2-associated congenital malformations Development (Cambridge, England), 150(3), dev201038. . PMCID: 10112907.

8.) Diegel CR, Hann S, Ayturk UM, Hu JCW, Lim KE, Droscha CJ, Madaj ZB, Foxa GE, Izaguirre I, Transgenics Core VVA, Paracha N, Pidhaynyy B, Dowd TL, Robling AG, Warman ML, Williams BO (2020 May). An osteocalcin-deficient mouse strain without endocrine abnormalities. PLoS genetics, 16(5), e1008361. doi: 10.1371/journal.pgen.1008361. ID: 32463812.

9.) Dischinger PS, Tovar EA, Essenburg CJ, Madaj ZB, Gardner EE, Callaghan ME, Turner AN, Challa AK, Kempston T, Eagleson B, Kesterson RA, Bronson RT, Bowman MJ, Graveel CR, Steensma MR (2018). <i>NF1</i> deficiency correlates with estrogen receptor signaling and diminished survival in breast cancer. NPJ breast cancer, 4(), 29. doi: 10.1038/s41523-018-0080-8. ID: 30182054.

Description:

The mission of the VAI Transgenic Core is to provide genetically modified animal models for our collaborators in support of their research. When these models are preexisting this provision can take the form of rederivation or reconstitution for the purpose of importing animals into our Vivarium’s barrier. In the absence of preexisting models our Core works closely with partnering labs to generate their ideal mutation de novo, often using CRISPR technology. Usually beginning with a brief consultation, this process involves discussing factors such as ideal background strain, molecular methods for optimizing the intended genetic change, mutation induction strategies, screening approaches and eventual QC of the new mutant line with the overall goal of giving our collaborators a fully informed, realistic picture of their projects timeframe, pitfalls and workflow. Additionally, our Core provides mouse sperm and embryo cryopreservation services for the purpose of archiving and distributing valuable lines. The Van Andel Institute is an AAALAC-accredited facility. All procedures are conducted according to the NIH Guide for the Care and Use of Laboratory Animals. We understand how important excellence in animal care is for producing quality research and are committed to the highest quality standards in pursuit of this principle.