Van Andel Institute
Dan Rohrer
333 Bostwick Ave NE
Grand Rapids, MI 49503
https://pbc.vai.org/
RRID: RRID:SCR_022912
BioBanking
Biorepository
Blood Processing
CDNA Synthesis
Cryostat Histology
DNA Isolation
H&E Staining
Histology
Human Pathology Services - Diagnostics & Consultation
Immunofluorescence
Immunohistochemistry
Nucleic Acid Extraction
Prepared Slides
Processing & Embedding- Histology
Processing & Embedding- Histology
Repository
RNA Integrity
RNA Isolation
Sample Accessioning
Sample Preparation
Scanning 20X Brightfield
Scanning 40X Brightfield
Slide Scanning
Special Stains Histology
Staining
Tissue Embed
Tissue MicroArray
Whole Slide Imaging
1.) Beddows I, Djirackor S, Omran DK, Jung E, Shih NN, Roy R, Hechmer A, Olshen A, Adelmant G, Tom A, Morrison J, Adams M, Rohrer DC, Schwartz LE, Pearce CL, Auman H, Marto JA, Drescher CW, Drapkin R, Shen H (2025 Mar 26). Impact of BRCA mutations, age, surgical indication, and hormone status on the molecular phenotype of the human Fallopian tube Nature Communications, 16(), 2981. . PMCID: 11947093.
2.) Xue Z, Xuan H, Lau K, Su Y, Wegener M, Li K, Turner L, Adams M, Shi X, Wen H (2025 Mar 14). Expression of ENL YEATS domain tumor mutations in nephrogenic or stromal lineage impairs kidney development Nature Communications, 16(), 2531. . PMCID: 11909213.
3.) Panzeri I, Fagnocchi L, Apostle S, Tompkins M, Wolfrum E, Madaj Z, Hostetter G, Liu Y, Schaefer K, Yang CH, Bergsma A, Drougard A, Dror E, Chandler DP, Schramek D, Triche TJ Jr, Pospisilik JA (2025 Jan 24). TRIM28-dependent developmental heterogeneity determines cancer susceptibility through distinct epigenetic states Nature Cancer, 6(2), 385-403. . PMCID: 11864977.
4.) Vatsa N, Brynildsen JK, Goralski TM, Kurgat K, Meyerdirk L, Breton L, DeWeerd D, Brasseur L, Turner L, Becker K, Gallik KL, Bassett DS, Henderson MX (2024 Oct 22). Network analysis of α-synuclein pathology progression reveals p21-activated kinases as regulators of vulnerability bioRxiv, (), 2024.10.22.619411. . PMCID: 11526907.
5.) Panzeri I, Madaj Z, Fagnocchi L, Apostle S, Tompkins M, Hostetter G, Pospisilik JA (2024 Oct 16). Chronic obesity does not alter cancer survival in Tp53R270H/+ mice bioRxiv, (), 2024.10.14.618190. . PMCID: 11507782.
6.) Panzeri I, Madaj Z, Fagnocchi L, Apostle S, Tompkins M, Hostetter G, Pospisilik JA (2024 Oct 16). Chronic obesity does not alter cancer survival in Tp53 (R270H/+) mice. bioRxiv : the preprint server for biology, (), . . PMID: 39463991.
7.) Longo J, DeCamp LM, Oswald BM, Teis R, Reyes-Oliveras A, Dahabieh MS, Ellis AE, Vincent MP, Damico H, Gallik KL, Compton SE, Capan CD, Williams KS, Esquibel CR, Madaj ZB, Lee H, Roy DG, Krawczyk CM, Haab BB, Sheldon RD, Jones RG (2024 Oct 14). Glucose-dependent glycosphingolipid biosynthesis fuels CD8(+) T cell function and tumor control. bioRxiv : the preprint server for biology, (), . . PMID: 39464161.
8.) Xue Z, Qin L, Xuan H, Luo K, Huang M, Xie L, Su Y, Xu L, Harsh J, Dale B, Shi X, Chen X, Kaniskan HÜ, Jin J, Wen H (2024 Aug 28). A potent and selective ENL degrader suppresses oncogenic gene expression and leukemia progression Science Advances, 10(35), eado1432. . PMCID: 11352836.
9.) Goralski TM, Meyerdirk L, Breton L, Brasseur L, Kurgat K, DeWeerd D, Turner L, Becker K, Adams M, Newhouse DJ, Henderson MX (2024 Mar 26). Spatial transcriptomics reveals molecular dysfunction associated with cortical Lewy pathology Nature Communications, 15(), 2642. . PMCID: 10966039.
10.) Lubben N, Brynildsen JK, Webb CM, Li HL, Leyns CE, Changolkar L, Zhang B, Meymand ES, O’Reilly M, Madaj Z, DeWeerd D, Fell MJ, Lee VM, Bassett DS, Henderson MX (2024 Mar 4). LRRK2 kinase inhibition reverses G2019S mutation-dependent effects on tau pathology progression Translational Neurodegeneration, 13(), 13. . PMCID: 10910783.
11.) Yue F, Ku AT, Stevens PD, Michalski MN, Jiang W, Tu J, Shi Z, Dou Y, Wang Y, Feng XH, Hostetter G, Wu X, Huang S, Shroyer NF, Zhang B, Williams BO, Liu Q, Lin X, Li Y (2024 Jan 11). Loss of ZNRF3/RNF43 Unleashes EGFR in Cancer bioRxiv, (), 2024.01.10.574969. . PMCID: 10802575.
12.) Diegel CR, Michalski MN, Williams BO (2023 Oct 15). β-catenin-dependent High Bone Mass Induced by Loss of APC in Osteoblasts Does Not Require Lrp5 or Lrp6 microPublication Biology, 2023(), 10.17912/micropub.biology.001000. . PMCID: 10613880.
13.) Panzeri I, Fagnocchi L, Apostle S, Tompkins M, Wolfrum E, Madaj Z, Hostetter G, Liu Y, Schaefer K, Chih-Hsiang Y, Bergsma A, Drougard A, Dror E, Chandler D, Schramek D, Triche TJ Jr, Pospisilik JA (2023 Sep 15). Developmental priming of cancer susceptibility bioRxiv, (), 2023.09.12.557446. . PMCID: 10515831.
14.) Diegel CR, Kramer I, Moes C, Foxa GE, McDonald MJ, Madaj ZB, Guth S, Liu J, Harris JL, Kneissel M, Williams BO (2023 Aug 24). Inhibiting WNT secretion reduces high bone mass caused by Sost loss-of-function or gain-of-function mutations in Lrp5 Bone Research, 11(), 47. . PMCID: 10447437.
15.) Goralski T, Meyerdirk L, Breton L, Brasseur L, Kurgat K, DeWeerd D, Turner L, Becker K, Adams M, Newhouse D, Henderson MX (2023 May 17). Spatial transcriptomics reveals molecular dysfunction associated with Lewy pathology bioRxiv, (), 2023.05.17.541144. . PMCID: 10245657.
16.) Bagchi A, Beddows I, Cornelius A, Robinson GW, Jewell SD (2022 May). Rare cases of medulloblastoma with hypermutation. Cancer reports (Hoboken, N.J.), 5(5), e1521. doi: 10.1002/cnr2.1521. ID: 34351088.
17.) Cao L, Huang C, Cui Zhou D, Hu Y, Lih TM, Savage SR, Krug K, Clark DJ, Schnaubelt M, Chen L, da Veiga Leprevost F, Eguez RV, Yang W, Pan J, Wen B, Dou Y, Jiang W, Liao Y, Shi Z, Terekhanova NV, Cao S, Lu RJ, Li Y, Liu R, Zhu H, Ronning P, Wu Y, Wyczalkowski MA, Easwaran H, Danilova L, Mer AS, Yoo S, Wang JM, Liu W, Haibe-Kains B, Thiagarajan M, Jewell SD, Hostetter G, Newton CJ, Li QK, Roehrl MH, Fenyö D, Wang P, Nesvizhskii AI, Mani DR, Omenn GS, Boja ES, Mesri M, Robles AI, Rodriguez H, Bathe (2021 Sep 16). Proteogenomic characterization of pancreatic ductal adenocarcinoma. Cell, 184(19), 5031-5052.e26. . ID: 34534465.
18.) Satpathy S, Krug K, Jean Beltran PM, Savage SR, Petralia F, Kumar-Sinha C, Dou Y, Reva B, Kane MH, Avanessian SC, Vasaikar SV, Krek A, Lei JT, Jaehnig EJ, Omelchenko T, Geffen Y, Bergstrom EJ, Stathias V, Christianson KE, Heiman DI, Cieslik MP, Cao S, Song X, Ji J, Liu W, Li K, Wen B, Li Y, Gümüş ZH, Selvan ME, Soundararajan R, Visal TH, Raso MG, Parra ER, Babur Ö, Vats P, Anand S, Schraink T, Cornwell M, Rodrigues FM, Zhu H, Mo CK, Zhang Y, da Veiga Leprevost F, Huang C, Chinnaiyan AM, Wyczalko (2021 Aug 5). A proteogenomic portrait of lung squamous cell carcinoma. Cell, 184(16), 4348-4371.e40. . ID: 34358469.
19.) Bagchi A, Madaj Z, Engel KB, Guan P, Rohrer DC, Valley DR, Wolfrum E, Feenstra K, Roche N, Hostetter G, Moore HM, Jewell SD (2021 May). Impact of Preanalytical Factors on the Measurement of Tumor Tissue Biomarkers Using Immunohistochemistry. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 69(5), 297-320. doi: 10.1369/0022155421995600. ID: 33641490.
20.) Wang LB, Karpova A, Gritsenko MA, Kyle JE, Cao S, Li Y, Rykunov D, Colaprico A, Rothstein JH, Hong R, Stathias V, Cornwell M, Petralia F, Wu Y, Reva B, Krug K, Pugliese P, Kawaler E, Olsen LK, Liang WW, Song X, Dou Y, Wendl MC, Caravan W, Liu W, Cui Zhou D, Ji J, Tsai CF, Petyuk VA, Moon J, Ma W, Chu RK, Weitz KK, Moore RJ, Monroe ME, Zhao R, Yang X, Yoo S, Krek A, Demopoulos A, Zhu H, Wyczalkowski MA, McMichael JF, Henderson BL, Lindgren CM, Boekweg H, Lu S, Baral J, Yao L, Stratton KG, Bramer (2021 Apr 12). Proteogenomic and metabolomic characterization of human glioblastoma. Cancer cell, 39(4), 509-528.e20. . ID: 33577785.
21.) Huang C, Chen L, Savage SR, Eguez RV, Dou Y, Li Y, da Veiga Leprevost F, Jaehnig EJ, Lei JT, Wen B, Schnaubelt M, Krug K, Song X, Cieślik M, Chang HY, Wyczalkowski MA, Li K, Colaprico A, Li QK, Clark DJ, Hu Y, Cao L, Pan J, Wang Y, Cho KC, Shi Z, Liao Y, Jiang W, Anurag M, Ji J, Yoo S, Zhou DC, Liang WW, Wendl M, Vats P, Carr SA, Mani DR, Zhang Z, Qian J, Chen XS, Pico AR, Wang P, Chinnaiyan AM, Ketchum KA, Kinsinger CR, Robles AI, An E, Hiltke T, Mesri M, Thiagarajan M, Weaver AM, Sikora AG, Lu (2021 Mar 8). Proteogenomic insights into the biology and treatment of HPV-negative head and neck squamous cell carcinoma. Cancer cell, 39(3), 361-379.e16. . ID: 33417831.
22.) Jiang L, Wang M, Lin S, Jian R, Li X, Chan J, Dong G, Fang H, Robinson AE, Snyder MP (2020 Oct 1). A Quantitative Proteome Map of the Human Body. Cell, 183(1), 269-283.e19. . ID: 32916130.
23.) Ferraro NM, Strober BJ, Einson J, Abell NS, Aguet F, Barbeira AN, Brandt M, Bucan M, Castel SE, Davis JR, Greenwald E, Hess GT, Hilliard AT, Kember RL, Kotis B, Park Y, Peloso G, Ramdas S, Scott AJ, Smail C, Tsang EK, Zekavat SM, Ziosi M, Aradhana, Ardlie KG, Assimes TL, Bassik MC, Brown CD, Correa A, Hall I, Im HK, Li X, Natarajan P, Lappalainen T, Mohammadi P, Montgomery SB, Battle A (2020 Sep 11). Transcriptomic signatures across human tissues identify functional rare genetic variation. Science (New York, N.Y.), 369(6509), . . ID: 32913073.
24.) Kim-Hellmuth S, Aguet F, Oliva M, Muñoz-Aguirre M, Kasela S, Wucher V, Castel SE, Hamel AR, Viñuela A, Roberts AL, Mangul S, Wen X, Wang G, Barbeira AN, Garrido-Martín D, Nadel BB, Zou Y, Bonazzola R, Quan J, Brown A, Martinez-Perez A, Soria JM, Getz G, Dermitzakis ET, Small KS, Stephens M, Xi HS, Im HK, Guigó R, Segrè AV, Stranger BE, Ardlie KG, Lappalainen T (2020 Sep 11). Cell type-specific genetic regulation of gene expression across human tissues. Science (New York, N.Y.), 369(6509), . . ID: 32913075.
25.) (2020 Sep 11). The GTEx Consortium atlas of genetic regulatory effects across human tissues. Science (New York, N.Y.), 369(6509), 1318-1330. . ID: 32913098.
26.) Gillette MA, Satpathy S, Cao S, Dhanasekaran SM, Vasaikar SV, Krug K, Petralia F, Li Y, Liang WW, Reva B, Krek A, Ji J, Song X, Liu W, Hong R, Yao L, Blumenberg L, Savage SR, Wendl MC, Wen B, Li K, Tang LC, MacMullan MA, Avanessian SC, Kane MH, Newton CJ, Cornwell M, Kothadia RB, Ma W, Yoo S, Mannan R, Vats P, Kumar-Sinha C, Kawaler EA, Omelchenko T, Colaprico A, Geffen Y, Maruvka YE, da Veiga Leprevost F, Wiznerowicz M, Gümüş ZH, Veluswamy RR, Hostetter G, Heiman DI, Wyczalkowski MA, Hiltke T, (2020 Jul 9). Proteogenomic Characterization Reveals Therapeutic Vulnerabilities in Lung Adenocarcinoma. Cell, 182(1), 200-225.e35. . ID: 32649874.
27.) Dou Y, Kawaler EA, Cui Zhou D, Gritsenko MA, Huang C, Blumenberg L, Karpova A, Petyuk VA, Savage SR, Satpathy S, Liu W, Wu Y, Tsai CF, Wen B, Li Z, Cao S, Moon J, Shi Z, Cornwell M, Wyczalkowski MA, Chu RK, Vasaikar S, Zhou H, Gao Q, Moore RJ, Li K, Sethuraman S, Monroe ME, Zhao R, Heiman D, Krug K, Clauser K, Kothadia R, Maruvka Y, Pico AR, Oliphant AE, Hoskins EL, Pugh SL, Beecroft SJI, Adams DW, Jarman JC, Kong A, Chang HY, Reva B, Liao Y, Rykunov D, Colaprico A, Chen XS, Czekański A, Jędryka (2020 Feb 20). Proteogenomic Characterization of Endometrial Carcinoma. Cell, 180(4), 729-748.e26. . ID: 32059776.
28.) Clark DJ, Dhanasekaran SM, Petralia F, Pan J, Song X, Hu Y, da Veiga Leprevost F, Reva B, Lih TM, Chang HY, Ma W, Huang C, Ricketts CJ, Chen L, Krek A, Li Y, Rykunov D, Li QK, Chen LS, Ozbek U, Vasaikar S, Wu Y, Yoo S, Chowdhury S, Wyczalkowski MA, Ji J, Schnaubelt M, Kong A, Sethuraman S, Avtonomov DM, Ao M, Colaprico A, Cao S, Cho KC, Kalayci S, Ma S, Liu W, Ruggles K, Calinawan A, Gümüş ZH, Geiszler D, Kawaler E, Teo GC, Wen B, Zhang Y, Keegan S, Li K, Chen F, Edwards N, Pierorazio PM, Chen X (2020 Jan 9). Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma. Cell, 180(1), 207. . ID: 31923397.
29.) Paulauskis JD, Blanc VM, Carey T, Chesla DW, Frey RC, Geddes T, Keats J, Loup A, Pruetz B, Rohrer DC, Valley DR, Tomlinson T, Akervall J, Wilson GD, Jewell SD (2019 Dec). Great Lakes Biorepository Research Network Biopreservation and biobanking, 17(6), 598-602. doi: 10.1089/bio.2019.0022. ID: 31211591.
30.) McCall SJ, Branton PA, Blanc VM, Dry SM, Gastier-Foster JM, Harrison JH, Jewell SD, Dash RC, Obeng RC, Rose J, Mateski DL, Liubinskas A, Robb JA, Ramirez NC, Shea K (2018 Feb). The College of American Pathologists Biorepository Accreditation Program: Results from the First 5 Years. Biopreservation and biobanking, 16(1), 16-22. doi: 10.1089/bio.2017.0108. ID: 29394087.
31.) Tan MH, Li Q, Shanmugam R, Piskol R, Kohler J, Young AN, Liu KI, Zhang R, Ramaswami G, Ariyoshi K, Gupte A, Keegan LP, George CX, Ramu A, Huang N, Pollina EA, Leeman DS, Rustighi A, Goh YPS, Chawla A, Del Sal G, Peltz G, Brunet A, Conrad DF, Samuel CE, O (2017 Oct 11). Dynamic landscape and regulation of RNA editing in mammals. Nature, 550(7675), 249-254. . ID: 29022589.
32.) Battle A, Brown CD, Engelhardt BE, Montgomery SB (2017 Oct 11). Genetic effects on gene expression across human tissues. Nature, 550(7675), 204-213. . ID: 29022597.
33.) Tukiainen T, Villani AC, Yen A, Rivas MA, Marshall JL, Satija R, Aguirre M, Gauthier L, Fleharty M, Kirby A, Cummings BB, Castel SE, Karczewski KJ, Aguet F, Byrnes A, Lappalainen T, Regev A, Ardlie KG, Hacohen N, MacArthur DG (2017 Oct 11). Landscape of X chromosome inactivation across human tissues. Nature, 550(7675), 244-248. . ID: 29022598.
VAI’s Pathology and Biorepository Core (PBC) integrates anatomic pathology expertise with biorepository and biospecimen science for investigator- and project-driven research. The Core builds upon strengths in standard histology, microscopy, molecular processing, and biobanking, along with novel technologies to test and apply best practices in biospecimen science. The Core’s focus on pathology and molecular quality metrics provide complementary emphasis on high-quality biospecimens and interpretable results with which to validate experimental models and extend them to clinical samples, thereby advancing the Institute’s mission to improve the health and enhance the lives of current and future generations.
The Pathology and Biorepository Core is led by Dr. Scott Jewell, who has extensive expertise in experimental pathology, immunology and biobanking, and Associate Director of Pathology Dr. Galen Hostetter, a board-certified pathologist with NIH fellowship training in medical genetics. The PBC has been accredited by the College of American Pathologists (CAP) since 2012.
