University of Delaware

Contact Info:

Arit Ghosh

77 E Delaware Avenue

Life Science Research Facility

Newark, DE 19711

https://bioimaging.dbi.udel.edu/flow-cytometry-core-2/

Grants and Identifiers:

RRID: RRID:SCR_022861

Instrumentation:

Other Equipment

FACSAria Fusion high-speed cell sorter and analyzer BD Rhapsody Express Cell Processing Unit VetScan HM5c veterinary hematology analyzer Nexcelom Cellometer Vision cell counter Zeiss Axiovert 200 inverted fluorescent microscope Thermo Shandon Cytospin 4 Beckman Spinchron DLX centrifuge

Services Provided:

FACS Cell Sorting

Flow Cytometric Analysis

Flow Cytometry Data Analysis

High Dimensional Flow Cytometry Analysis

Relevant Publications:

1.) Ghosh A, Coffin M, West R, Fowler VM (2022 Mar). Erythroid differentiation in mouse erythroleukemia cells depends on Tmod3-mediated regulation of actin filament assembly into the erythroblast membrane skeleton. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36(3), e22220. doi: 10.1096/fj.202101011R. ID: 35195928.

2.) Rondelli CM, Perfetto M, Danoff A, Bergonia H, Gillis S, O (2021 Aug). The ubiquitous mitochondrial protein unfoldase CLPX regulates erythroid heme synthesis by control of iron utilization and heme synthesis enzyme activation and turnover. The Journal of biological chemistry, 297(2), 100972. . ID: 34280433.

3.) Ghosh A, Fowler VM (2021 May 24). Tropomodulins. Current biology : CB, 31(10), R501-R503. . ID: 34033779.

4.) Pal K, Nowak R, Billington N, Liu R, Ghosh A, Sellers JR, Fowler VM (2020 May 21). Megakaryocyte migration defects due to nonmuscle myosin IIA mutations underlie thrombocytopenia in MYH9-related disease. Blood, 135(21), 1887-1898. . ID: 32315395.

Description:

The Flow Cytometry Core Facility, located at the University of Delaware Main campus (UD) in Newark, Delaware, provides a wide variety of flow cytometry and hematology services for internal and external investigators. The Core facility is equipped with – • Two 15-color FACSAria Fusion high-speed cell sorter, • BD Rhapsody Express Cell Processing Unit, • VetScan HM5c veterinary hematology analyzer, • Nexcelom Cellometer Vision cell counter, • Zeiss Axiovert 200 inverted fluorescent microscope, • Thermo Shandon Cytospin 4 & a Beckman Spinchron DLX centrifuge, • General Laboratory facilities for Mammalian Cell Culture and Sample preparation, • Laminar flow BSL-2 biosafety cabinets, microscopes and centrifuges.