Specimen collection by gravity contact-free and contamination-free. Laser Microdissection (LMD) makes it possible to distinguish between relevant and non-relevant cells or tissues. It enables the researcher to obtain homogeneous, ultra-pure samples from heterogeneous starting material. Researcher can selectively and routinely analyze regions of interest down to single cells from all kinds of tissues, even living cells from cell culture, to obtain results that are relevant, reproducible, and specific. [Product Link]
Leica TCS SP2 is a spectral confocal laser scanning microscope with 405 UV laser 3CHDIC digital emission 400-800nm range designed to acquire high spatial resolution images of fluorescently labeled materials and for analysis of these images. The confocal principle utilizes a pinhole (confocal aperture) to eliminate out-of-focus light from fluorescently labeled specimens (i.e., provide “optical sectioning�). Lasers provide intense, point illumination that is scanned over the preparation and the fluorescence at each point is quantified and used to construct a representation of the object brightness (an image). This method provides high resolution for the x and y planes as well as vertically (z plane). The SP2 is an advanced confocal system with nine laser excitation lines spanning the spectrum from UV to near IR. The following laser lines are available: 405nm, 458, 476, 488, 496, 514, 543, 594, and 633, all fiber coupled and controlled by AOTFs. [Product Link]
Leica TCS SP5 is a broadband confocal microscope that provides the full range of scan speeds at the a high resolution. With its SP detection (five channels simultaneously) and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. The system is also the platform for the new Leica DM6000 CFS (Confocal Fixed Stage) for physiological and electrophysiological experiments and for the new super resolution Leica TCS STED confocal microscope. [Product Link]
ALMS is a Leica Microsystems Center for Excellence and provides microscopy services, consultation, and support for the application of novel microscopic and spectroscopic methods and advanced image analysis techniques for the study of macromolecules, cellular dynamics and nano-scale characterization of bio-materials. The facility provides a collection of high-level, customized biological fluorescence microscopes and small-animal imaging devices that provide the ability to study biological processes with high spatial and temporal resolution in whole organisms and in living cells down to the single molecule detection level with nanometer-accuracy. Located on the basement and second floors of the CNSI building, two optical suites of 1,000 square feet each were specifically designed to house our microscopes with the required environment control (low vibration, air-filtered, air-conditioned to ±1°C and light-tight) and services. The facility currently provides the following services: Wide-field Fluorescence Imaging Microscopy (on a limited basis), Confocal One-Photon and Two-Photon Laser Scanning Microscopy, (both point scanning and spinning disk), Fluorescence Correlation Spectroscopy (FCS), Fluorescence Resonance Energy Transfer (FRET), microscopic and macroscopic Fluorescence Lifetime Imaging (FLIM) with Time-Correlated-Single-Photon-Counting (TCSPC) and Near-Infrared (NIR) Detection, Stimulated Emission Depletion laser-scanning microscopy (STED) (a super-resolution technique), both microscopic and macroscopic (small animal) spectral unmixing and laser capture microdissection.
