Instituto Nacional de Cancerología
Alejandro Lopez-Saavedra
Ave. San Fernando 22, Belisario Domínguez Sección 16, Tlalpan, 14080
Ciudad de Mexico
https://www.admiramicro.com
RRID: RRID:SCR_022788
ZEISS Axio Scan.Z1 Slide Scanner
Digitize your specimens with the Axio Scan.Z1 slide scanner and create high-quality virtual slides. Axio Scan.Z1 tackles the most demanding virtual microscopy research tasks as easily as it handles your routine work. The software module ZEN slidescan is designed specifically for the workflow of capturing virtual slides, while ZEN image analysis tools prepare your data accurately. Organize your virtual slides with ZEN browser, the web-based database, then view your data from any location using any operating system , or share virtual microscopy images online with colleagues and organize your projects, even when you are on the go. [Product Link]
RRID:SCR_020927
Zeiss Axio Vert series Axiovert 200 inverted microscope
Inverted microscope that is used for the examination of cell and tissue culture of sediments in culture flasks, Petri dishes, microtiter plates, etc. in transmitted and reflected light. It hass attachment possibilities for various tools (micromanipulation), different light sources, temperature control devices. [Product Link]
RRID:SCR_020915
Zeiss Lightsheet Z.1 Lightsheet Fluorescence Microscope
Light sheet fluorescence microscope for the imaging of large cleared specimens. Lightsheet Z.1 with Clr Plan-Apochromat 20x/1.0 Corr nd=1.38 is used to perform experiments with tissue cleared by Scale medium (Hama et al, Nat Neurosci. 2011), which has a refractive index of n=1.38. [Product Link]
RRID:SCR_020919
Zeiss LSM 710 Confocal Inverted Microscope
LSM 710 is a confocal is an inverted microscope that enables confocal microscopy for a wide variety of applications. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. [Product Link]
RRID:SCR_018063
Zeiss PALM MicroBeam PALM
PALM MicroBeam scanning electron microscope for isolating uncontaminated source material simple. [Product Link]
RRID:SCR_020929
Zeiss PALM MicroTweezers
Optical tweezers system for contact free cell manipulation as well as to trap, move, and sort microscopic particles, sort live cells, organelles, and other large biomolecules. [Product Link]
RRID:SCR_018647
Metasystems METAFER
Software coupled with an AxioIimager.Z2 for whole slide scanning. Designed for imaging mitotic cells and karyotyping
ZEISS AURIGA
FIB STEM system
ZEISS AxioImager.A2
Upright microscope. Brightfield, Phase contrast, Dark field, Epifluorescence. Coupled with an Axiocam ICC5
ZEISS AxioImager.Z2 with METAFER
Upright microscope with motorized stage for scanning through Metasystem software (mitotic cells, ABL/BCR translocation/ ISIS software for karyotyping
ZEISS Axioskop2 Mot Plus
Upright microscope, motorized stage only in Z direction. Brightfield, Darkfield, Phase Contrast, Epifluorescence. Coupled with an AxioCam 305
ZEISS AxioVert A1
Inverted microscope. Brightfield, Phase contrast, Epifluorescence (Led). Coupled with an AxioCam 305
ZEISS Discovery.V12
Stereomicroscope with an Axiocam 305. Objectives: PlanApo S 0.63X, Plan S 1.0X, and PlanApo S 1.5X
ZEISS ELYRA PS.1
Super-resolution PALM/STORM/SIM/TIRF
Analysis Workstations
Cell Culture
Cell Imaging
Confocal Microscopy
Correlative Light Electron Microscopy
Deconvolution
Electron Microscopy
F.I.S.H.
Focused Ion Beam (FIB)
Image Aquisition
Image Data Management
Image Processing And Analysis
Karyotyping
Laser Capture Microdissection
Light-sheet Microscopy
Live Cell Imaging
Microscopy
Multiphoton Microscopy
Multiplex Fluorescence
Prepared Slides
Sample Preparation
Scanning Electron Microscopy
Slide Scanning
Stereomicroscopy
Super-resolution Microscopy
Telomere Length Analysis
TIRF Microscopy
Training
Transmission Electron Microscopy
Whole Slide Imaging
Widefield Microscopy
1.) Martinez-Alarcon O, Colin-Lagos D, Ramirez-Meza X, Castilla A, Hernandez-Montes G, Flores-Garza E, Lopez-Saavedra A, Avila-Gonzalez D, Martinez-Juarez A, Molina-Hernández A, Diaz-Martinez NE, Portillo W, Diaz NF (2025 Feb 26). Prolactin drives cortical neuron maturation and dendritic development during murine embryonic stem cell differentiation Frontiers in Cell and Developmental Biology, 13(), 1551090. . PMCID: 11897521.
2.) Herrera-Quiterio GA, Valencia-González HA, de la Cruz-López KG, Fernández-Coto DL, Gil J, Marko-Varga G, Morales-Gálvez J, Sánchez NC, Rodríguez-Bautista R, Avilés-Salas A, Arrieta O, García-Carrancá A, Encarnación-Guevara S (2025 Jan 27). TMEM160 Promotes Tumor Growth in Lung Adenocarcinoma and Cervical Adenocarcinoma Cell Lines International Journal of Molecular Sciences, 26(3), 1097. . PMCID: 11816668.
3.) Magaña-Ávila G, Carbajal-Contreras H, Amnekar R, Dite T, Téllez-Sutterlin M, García-Ávila K, Marquina-Castillo B, Lopez-Saavedra A, Vazquez N, Rojas-Ortega E, Delpire E, Ellison DH, Alessi DR, Gamba G, Castañeda-Bueno M (2024 Dec 17). NRBP1 and TSC22D proteins impact distal convoluted tubule physiology through modulation of the WNK pathway bioRxiv, (), 2024.12.12.628222. . PMCID: 11702584.
4.) Torres-Betancourt JA, Hernández-Delgadillo R, Cauich-Rodríguez JV, Oliva-Rico DA, Solis-Soto JM, García-Cuellar CM, Sánchez-Pérez Y, Pineda-Aguilar N, Flores-Treviño S, Meester I, Nakagoshi-Cepeda SE, Arevalo-Niño K, Nakagoshi-Cepeda MA, Cabral-Romero C (2024 Oct 16). A Polyurethane Electrospun Membrane Loaded with Bismuth Lipophilic Nanoparticles (BisBAL NPs): Proliferation, Bactericidal, and Antitumor Properties, and Effects on MRSA and Human Breast Cancer Cells Journal of Functional Biomaterials, 15(10), 309. . PMCID: 11508964.
5.) Delgado-Waldo I, Dokudovskaya S, Loissell-Baltazar YA, Pérez-Arteaga E, Coronel-Hernández J, Martínez-Vázquez M, Pérez-Yépez EA, Lopez-Saavedra A, Jacobo-Herrera N, Pérez Plasencia C (2024 Oct 3). Laherradurin Inhibits Colorectal Cancer Cell Growth by Induction of Mitochondrial Dysfunction and Autophagy Induction Cells, 13(19), 1649. . PMCID: 11475353.
6.) De la Cruz-López KG, Alvarado-Ortiz E, Valencia-González HA, Beltrán-Anaya FO, Zamora-Fuentes JM, Hidalgo-Miranda A, Ortiz-Sánchez E, Espinal-Enríquez J, García-Carrancá A (2024 Jul 18). Metformin induces ZFP36 by mTORC1 inhibition in cervical cancer-derived cell lines BMC Cancer, 24(), 853. . PMCID: 11256429.
7.) Carbajal-Contreras H, Murillo-de-Ozores AR, Magaña-Avila G, Marquez-Salinas A, Bourqui L, Tellez-Sutterlin M, Bahena-Lopez JP, Cortes-Arroyo E, Behn-Eschenburg SG, Lopez-Saavedra A, Vazquez N, Ellison DH, Loffing J, Gamba G, Castañeda-Bueno M (2024 Feb 1). Arginine vasopressin regulates the renal Na(+)-Cl(-) and Na(+)-K(+)-Cl(-) cotransporters through with-no-lysine kinase 4 and inhibitor 1 phosphorylation. American journal of physiology. Renal physiology, 326(2), F285-F299. doi: 10.1152/ajprenal.00343.2023. PMID: 38096266.
8.) Justo-Garrido M, López-Saavedra A, Alcaraz N, Cortés-González CC, Oñate-Ocaña LF, Caro-Sánchez CH, Castro-Hernández C, Arriaga-Canon C, Díaz-Chávez J, Herrera LA (2023 Nov 9). Association of SLC12A1 and GLUR4 Ion Transporters with Neoadjuvant Chemoresistance in Luminal Locally Advanced Breast Cancer International Journal of Molecular Sciences, 24(22), 16104. . PMCID: 10670992.
9.) Ortiz-Pedraza Y, Muñoz-Bello JO, Ramos-Chávez LA, Martínez-Ramírez I, Olmedo-Nieva L, Manzo-Merino J, López-Saavedra A, Pérez-de la Cruz V, Lizano M (2023 Jan 24). HPV16 E6 and E7 Oncoproteins Stimulate the Glutamine Pathway Maintaining Cell Proliferation in a SNAT1-Dependent Fashion Viruses, 15(2), 324. . PMCID: 9964736.
10.) Colin-Val Z, Vera-Márquez CD, Herrera-Rodríguez MA, Del Pilar Ramos-Godinez M, López-Saavedra A, Cano-Martínez A, Robledo-Cadena DX, Rodríguez-Enríquez S, Correa F, Delgado-Buenrostro NL, Chirino YI, López-Marure R (2022 Aug). Titanium Dioxide (E171) Induces Toxicity in H9c2 Rat Cardiomyoblasts and Ex Vivo Rat Hearts. Cardiovascular toxicology, 22(8), 713-726. doi: 10.1007/s12012-022-09747-5. ID: 35633469.
11.) Martínez-González JJ, Ríos-Morales SL, Guevara-Flores A, Ramos-Godinez MDP, López-Saavedra A, Rendón JL, Del Arenal Mena IP (2022 Aug). Evaluating the effect of curcumin on the metacestode of Taenia crassiceps. Experimental parasitology, 239(), 108319. . ID: 35777452.
12.) Murillo-de-Ozores AR, Carbajal-Contreras H, Magaña-Ávila GR, Valdés R, Grajeda-Medina LI, Vázquez N, Zariñán T, López-Saavedra A, Sharma A, Lin DH, Wang WH, Delpire E, Ellison DH, Gamba G, Castañeda-Bueno M (2022 Jul 21). Multiple molecular mechanisms are involved in the activation of the kidney sodium-chloride cotransporter by hypokalemia. Kidney international, (), . . ID: 35870644.
13.) Fabian-Morales E, Vallejo-Escamilla D, Gudiño A, Rodríguez A, González-Barrios R, Rodríguez Torres YL, Castro Hernández C, de la Torre-Luján AH, Oliva-Rico DA, Ornelas Guzmán EC, López Saavedra A, Frias S, Herrera LA (2022 May 1). Large-scale topological disruption of chromosome territories 9 and 22 is associated with nonresponse to treatment in CML. International journal of cancer, 150(9), 1455-1470. doi: 10.1002/ijc.33903. ID: 34913480.
14.) Cáceres-Gutiérrez RE, Andonegui MA, Oliva-Rico DA, González-Barrios R, Luna F, Arriaga-Canon C, López-Saavedra A, Prada D, Castro C, Parmentier L, Díaz-Chávez J, Alfaro-Mora Y, Navarro-Delgado EI, Fabian-Morales E, Tran B, Shetty J, Zhao Y, Alcaraz N, De la Rosa C, Reyes JL, Hédouin S, Hubé F, Francastel C, Herrera LA (2022 Apr). Proteasome inhibition alters mitotic progression through the upregulation of centromeric α-Satellite RNAs. The FEBS journal, 289(7), 1858-1875. doi: 10.1111/febs.16261. ID: 34739170.
15.) Oliva-Rico D, Fabian-Morales E, Cáceres-Gutiérrez RE, Gudiño A, Cisneros-Soberanis F, Dominguez J, Almaraz-Rojas O, Arriaga-Canon C, Castro-Hernández C, De la Rosa C, Reyes JL, Herrera LA (2022 Mar 18). Methylation of Subtelomeric Chromatin Modifies the Expression of the lncRNA TERRA, Disturbing Telomere Homeostasis. International journal of molecular sciences, 23(6), . . ID: 35328692.
16.) Saldivar-Cerón HI, Villamar-Cruz O, Wells CM, Oguz I, Spaggiari F, Chernoff J, Patiño-López G, Huerta-Yepez S, Montecillo-Aguado M, Rivera-Pazos CM, Loza-Mejía MA, Vivar-Sierra A, Briseño-Díaz P, Zentella-Dehesa A, Leon-Del-Rio A, López-Saavedra A, Padierna-Mota L, Ibarra-Sánchez MJ, Esparza-López J, Hernández-Rivas R, Arias-Romero LE (2021). p21-Activated Kinase 1 Promotes Breast Tumorigenesis <i>via</i> Phosphorylation and Activation of the Calcium/Calmodulin-Dependent Protein Kinase II. Frontiers in cell and developmental biology, 9(), 759259. doi: 10.3389/fcell.2021.759259. ID: 35111748.
17.) Huerta-García E, Ramos-Godinez MDP, López-Saavedra A, Alfaro-Moreno E, Gómez-Crisóstomo NP, Colín-Val Z, Sánchez-Barrera H, López-Marure R (2019 Apr 15). Internalization of Titanium Dioxide Nanoparticles Is Mediated by Actin-Dependent Reorganization and Clathrin- and Dynamin-Mediated Endocytosis in H9c2 Rat Cardiomyoblasts. Chemical research in toxicology, 32(4), 578-588. doi: 10.1021/acs.chemrestox.8b00284. ID: 30730135.
18.) Guerra-De-Blas PDC, Bobadilla-Del-Valle M, Sada-Ovalle I, Estrada-García I, Torres-González P, López-Saavedra A, Guzmán-Beltrán S, Ponce-de-León A, Sifuentes-Osornio J (2019). Simvastatin Enhances the Immune Response Against <i>Mycobacterium tuberculosis</i>. Frontiers in microbiology, 10(), 2097. doi: 10.3389/fmicb.2019.02097. ID: 31616387.
19.) Muñoz-Bello JO, Olmedo-Nieva L, Castro-Muñoz LJ, Manzo-Merino J, Contreras-Paredes A, González-Espinosa C, López-Saavedra A, Lizano M (2018 Oct 13). HPV-18 E6 Oncoprotein and Its Spliced Isoform E6*I Regulate the Wnt/β-Catenin Cell Signaling Pathway through the TCF-4 Transcriptional Factor. International journal of molecular sciences, 19(10), . . ID: 30322153.
20.) Chirino YI, García-Cuellar CM, García-García C, Soto-Reyes E, Osornio-Vargas ÁR, Herrera LA, López-Saavedra A, Miranda J, Quintana-Belmares R, Pérez IR, Sánchez-Pérez Y (2017 Apr 15). Airborne particulate matter in vitro exposure induces cytoskeleton remodeling through activation of the ROCK-MYPT1-MLC pathway in A549 epithelial lung cells. Toxicology letters, 272(), 29-37. . ID: 28279687.
21.) López-Saavedra A, Ramírez-Otero M, Díaz-Chávez J, Cáceres-Gutiérrez R, Justo-Garrido M, Andonegui MA, Mendoza J, Downie-Ruíz Á, Cortés-González C, Reynoso N, Castro-Hernández C, Domínguez-Gómez G, Santibáñez M, Fabián-Morales E, Pruefer F, Luna-Maldonado F, González-Barrios R, Herrera LA (2016 Aug 2). MAD2γ, a novel MAD2 isoform, reduces mitotic arrest and is associated with resistance in testicular germ cell tumors. Cell cycle (Georgetown, Tex.), 15(15), 2066-76. doi: 10.1080/15384101.2016.1198863. ID: 27315568.
22.) Gómez EO, Chirino YI, Delgado-Buenrostro NL, López-Saavedra A, Meraz-Cruz N, López-Marure R (2016 Mar). Secretome derived from breast tumor cell lines alters the morphology of human umbilical vein endothelial cells. Molecular membrane biology, 33(1), 29-37. . ID: 27690154.
23.) Andonegui-Elguera MA, Cáceres-Gutiérrez RE, Luna-Maldonado F, López-Saavedra A, Díaz-Chávez J, Cisneros-Soberanis F, Prada D, Mendoza-Pérez J, Herrera LA (2016). BUB1 and SURVIVIN proteins are not degraded after a prolonged mitosis and accumulate in the nuclei of HCT116 cells. Cell death discovery, 2(), 16079. . ID: 27818790.
24.) Huerta-García E, Márquez-Ramírez SG, Ramos-Godinez Mdel P, López-Saavedra A, Herrera LA, Parra A, Alfaro-Moreno E, Gómez EO, López-Marure R (2015 Dec). Internalization of titanium dioxide nanoparticles by glial cells is given at short times and is mainly mediated by actin reorganization-dependent endocytosis. Neurotoxicology, 51(), 27-37. . ID: 26340880.
25.) Sánchez-Pérez Y, Chirino YI, Osornio-Vargas ÁR, Herrera LA, Morales-Bárcenas R, López-Saavedra A, González-Ramírez I, Miranda J, García-Cuellar CM (2014 Feb 10). Cytoplasmic p21(CIP1/WAF1), ERK1/2 activation, and cytoskeletal remodeling are associated with the senescence-like phenotype after airborne particulate matter (PM(10)) exposure in lung cells. Toxicology letters, 225(1), 12-9. . ID: 24291038.
26.) González-Barrios R, Soto-Reyes E, Quiroz-Baez R, Fabián-Morales E, Díaz-Chávez J, Del Castillo V, Mendoza J, López-Saavedra A, Castro C, Herrera LA (2014). Differential distribution of HP1 proteins after trichostatin a treatment influences chromosomal stability in HCT116 and WI-38 cells. Cell division, 9(), 6. doi: 10.1186/s13008-014-0006-2. ID: 25729403.
27.) Zepeta-Flores N, Valverde M, Lopez-Saavedra A, Rojas E (2018 Apr./Jun). Glutathione depletion triggers actin cytoskeleton changes via actin-binding proteins. Genetics and molecular biology, 41(2), 475-487. . ID: 29870570.
28.) García-Cuellar CM, Hernández-Delgadillo R, Torres-Betancourt JA, Solis-Soto JM, Meester I, Sánchez-Pérez Y, Pineda-Aguilar N, Nakagoshi-Cepeda SE, Sánchez-Nájera RI, Nakagoshi-Cepeda MAA, Chellam S, Cabral-Romero C (2023 Jan-Dec). Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer. Journal of applied biomaterials & functional materials, 21(), 22808000231161177. doi: 10.1177/22808000231161177. PMID: 36942951.
The Advanced Microscopy Applications Unit (ADMiRA) is an Imaging Core Facility located within the National Cancer Institute of Mexico. It provides scientific and technical support in imaging to diverse communities including academy, industry, health care, government sector, etc. Imaging technologies in ADMiRA include: Confocal, Widefield, Super-Resolution, Lightsheet, FIB-SEM, Laser Microdisection, and Automated digital scanning.
