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CRCHUM-Cell Imaging

University of Montreal

Contact Info:

Aurélie Cleret-Buhot

900, St Denis street

CHUM-Pavillon R

Montreal, QC H2X 0A9

https://www.chumontreal.qc.ca/crchum/plateformes-services/imagerie-cellulaire

Grants and Identifiers:

RRID: RRID:SCR_022726

Instrumentation:

Leica TCS SP5 Broadband Confocal Laser Scanning Microscope

Leica TCS SP5 is a broadband confocal microscope that provides the full range of scan speeds at the a high resolution. With its SP detection (five channels simultaneously) and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. The system is also the platform for the new Leica DM6000 CFS (Confocal Fixed Stage) for physiological and electrophysiological experiments and for the new super resolution Leica TCS STED confocal microscope. [Product Link]

RRID:SCR_020233

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Olympus FV1000 Confocal Microscope

Next generation imaging system designed for high resolution, confocal observation of both fixed and living cells.FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens.In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photoactivation. [Product Link]

RRID:SCR_020337

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Zeiss Spinning-Disc/TIRF

Inverted spinning-disc Confocal Microscope

Zeiss Zeiss LSM 900 with Airyscan2

Inverted Confocal Laser Scanning Microscope

Other Equipment

Leica DM4000B Zeiss AxioObserver Z1 Spinning-Disc Confocal Zeiss AxioObserver Z1 TIRF Olympus FV1000MPE Confocal Zeiss LSM900-Airyscan2

Services Provided:

3D & 4D Image Processing Stations

3D Visualization

Analysis Workstations

Cell Imaging

Confocal Microscopy

Confocal/ Super Resolution Microscope

Consultation

Consultations

Deconvolution

Fluorescence Microscope With Optical Sectioning Capability

Functional Brain Imaging

Image Aquisition

Image Processing And Analysis

Immunofluorescence

In-vivo Imaging

Live Cell Imaging

Microscopy

Multiphoton Microscopy

Multiphoton/ Harmonic Generation Microscope

Multiplex Fluorescence

Optical Imaging

TIRF Microscopy

Training

Relevant Publications:

1.) Lemaître F, Farzam-Kia N, Carmena Moratalla A, Carpentier Solorio Y, Clenet ML, Tastet O, Cleret-Buhot A, Guimond JV, Haddad E, Duquette P, Girard JM, Prat A, Larochelle C, Arbour N (2022 Sep 1). IL-27 shapes the immune properties of human astrocytes and their impact on encountered human T lymphocytes. Journal of neuroinflammation, 19(1), 212. doi: 10.1186/s12974-022-02572-1. ID: 36050707.


2.) Preston SEJ, Bartish M, Richard VR, Aghigh A, Gonçalves C, Smith-Voudouris J, Huang F, Thébault P, Cleret-Buhot A, Lapointe R, Légaré F, Postovit LM, Zahedi RP, Borchers CH, Miller WH Jr, Del Rincón SV (2022 Aug). Phosphorylation of eIF4E in the stroma drives the production and spatial organisation of collagen type I in the mammary gland. Matrix biology : journal of the International Society for Matrix Biology, 111(), 264-288. . ID: 35842012.


3.) Jamann H, Cui QL, Desu HL, Pernin F, Tastet O, Halaweh A, Farzam-Kia N, Mamane VH, Ouédraogo O, Cleret-Buhot A, Daigneault A, Balthazard R, Klement W, Lemaître F, Arbour N, Antel J, Stratton JA, Larochelle C (2022). Contact-Dependent Granzyme B-Mediated Cytotoxicity of Th17-Polarized Cells Toward Human Oligodendrocytes. Frontiers in immunology, 13(), 850616. doi: 10.3389/fimmu.2022.850616. ID: 35479072.


4.) Guo Q, Bartish M, Gonçalves C, Huang F, Smith-Voudouris J, Krisna SS, Preston SEJ, Emond A, Li VZ, Duerr CU, Gui Y, Cleret-Buhot A, Thebault P, Lefrère H, Lenaerts L, Plourde D, Su J, Mindt BC, Hewgill SA, Cotechini T, Hindmarch CCT, Yang W, Khoury E, Zhan Y, Narykina V, Wei Y, Floris G, Basik M, Amant F, Quail DF, Lapointe R, Fritz JH, Del Rincon SV, Miller WH Jr (2021 Jul 15). The MNK1/2-eIF4E Axis Supports Immune Suppression and Metastasis in Postpartum Breast Cancer. Cancer research, 81(14), 3876-3889. . ID: 33975880.


5.) Lemaître F, Carmena Moratalla A, Farzam-Kia N, Carpentier Solorio Y, Tastet O, Cleret-Buhot A, Guimond JV, Haddad E, Arbour N (2021). Capturing T Lymphocytes Frontiers in immunology, 12(), 668483. doi: 10.3389/fimmu.2021.668483. ID: 33968073.


6.) Flores Molina M, Fabre T, Cleret-Buhot A, Soucy G, Meunier L, Abdelnabi MN, Belforte N, Turcotte S, Shoukry NH (2020 Mar 25). Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment. Journal of visualized experiments : JoVE, (157), . doi: 10.3791/60740. ID: 32281982.


Description:

Optical and Fluorescence microscopy facility. From regular brightfield microscopes to high performence LSM900- Airyscan confocal microscope. Also Image Analysis with Imaris Full Spectrum and ClearDeconvolution software.