Mayo Clinic
Kevin Pavelko
200 First St. SW
Guggenheim Building, Room 527
Rochester, MN 55905
https://www.mayo.edu/research/core-resources/immune-monitoring-core/overview
RRID: RRID:SCR_022724
Bio-Rad S3e Cell Sorter
Cell sorter that allows performance of 1 to 4 color sorts and sorts large quantities of pure viable cells. [Product Link]
RRID:SCR_019710
FEI Hyperion II System
Electrical failure analysis system for semiconductors that offers transistor probing for electrical characterization and fault localization in support of semiconductor technology development, yield engineering and device reliability improvement. [Product Link]
RRID:SCR_019885
Fluidigm CyTOF Helios Mass Cytometer
Mass cytometer that enables deep profiling of translational and clinical research samples across a range of cell surface and intracellular markers. [Product Link]
RRID:SCR_019916
(Equipment No Longer Active At this Facility)
Fluidigm Helios Cell Mass Cytometer
Helios, a CyTOF system, comprises the most recent advances in mass cytometry and is designed to provide a new and improved tool for bioanalytical single-cell detection and analysis. This high-performance mass cytometer, from the CyTOF family of instruments, enables the analysis of more than 40 markers and ly allows their quantitative determination with negligible spectral overlap, a result of exquisite resolution between mass detection channels. Helios provides users with 135 detection channels that can simultaneously resolve multiple elemental probes at high acquisition rates, thereby maximizing the per-cell information obtained from a single sample. The expanded mass range of 75 209 amu and superior mass resolution provide researchers with the ability to differentiate adjacent peaks. The Helios system provides fast instrument startup, an easyto-use pneumatic sample loading system, an improved cell detection rate, and enhanced data storage capabilities. These attributes provide researchers with an unparalleled ability to generate high-resolution phenotypic and functional profiles of cells from normal and diseased states. [Product Link]
RRID:SCR_019917
Akoya Phenocycler-Fusion
The Akoya Biosciences Phenocycler-Fusion system is a high-speed cutting-edge imaging system that couples an automated fluidics control system — Phenocycler — with a high-resolution image scanning system — PhenoImager.
Fluidigm Hyperion Imaging System
Akoya PhenoCycler Fusion
Analysis Workstations
CyTOF
Data Analysis And Interpretation
FACS Cell Sorting
Flow Cytometry Data Analysis
Imaging Mass Cytomety
Immune Monitoring
Immunoassay
Mass Cytometry
Multiplexed Immunofluorescence
Phenotyping
Sample Preparation
Single-Cell Analysis
Slide Scanning
Spatial / Single Cell Data Analysis
Whole Slide Imaging
1.) Leon-Ferre RA, Whitaker KR, Suman VJ, Hoskin T, Giridhar KV, Moore RM, Al-Jarrad A, McLaughlin SA, Northfelt DW, Hunt KN, Conners AL, Moyer A, Carter JM, Kalari K, Weinshilboum R, Wang L, Ingle JN, Knutson KL, Ansell SM, Boughey JC, Goetz MP, Villasboas JC (2024 Jun 10). Pre-treatment peripheral blood immunophenotyping and response to neoadjuvant chemotherapy in operable breast cancer. Breast cancer research : BCR, 26(1), 97. . ID: 38858721.
2.) Khoury M, Guo Q, Furuta K, Correia C, Meroueh C, Kim Lee HS, Warasnhe K, Valenzuela-Pérez L, Mazar AP, Kim I, Noh YK, Holmes H, Romero MF, Sussman CR, Pavelko KD, Islam S, Bamidele AO, Hirsova P, Li H, Ibrahim SH (2024 Jun). Glycogen synthase kinase 3 activity enhances liver inflammation in MASH. JHEP reports : innovation in hepatology, 6(6), 101073. . ID: 38882600.
3.) Alexander MP, Zaidi M, Larson N, Mullan A, Pavelko KD, Stegall MD, Bentall A, Wouters BG, McKee T, Taner T (2024 Apr). Exploring the single-cell immune landscape of kidney allograft inflammation using imaging mass cytometry. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 24(4), 549-563. doi: 10.1016/j.ajt.2023.11.008. ID: 37979921.
4.) Parthasarathy G, Mauer AS, Golla N, Daniel PV, Kim LH, Sidhu GS, Marek GW 3rd, Loeuillard E, Krishnan A, Lee HSK, Pavelko KD, Charlton M, Hirsova P, Ilyas SI, Malhi H (2024 Feb 8). Macrophage RAGE activation is proinflammatory in NASH. JCI insight, 9(3), . . ID: 38175729.
5.) Krishnan A, Ozturk NB, Cutshaw KA, Guicciardi ME, Kitagataya T, Olson KE, Pavelko KD, Sherman W, Wixom AQ, Jalan-Sakrikar N, Baez-Faria M, Gutierrez F, Gores GJ (2024 Jan 25). Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) deletion in myeloid cells augments cholestatic liver injury. Scientific reports, 14(1), 2145. . ID: 38273071.
6.) Zanwar S, Jacob EK, Greiner C, Pavelko K, Strausbauch M, Anderson E, Arsana A, Weivoda M, Shah MV, Kourelis T (2023 Sep 26). The immunome of mobilized peripheral blood stem cells is predictive of long-term outcomes and therapy-related myeloid neoplasms in patients with multiple myeloma undergoing autologous stem cell transplant. Blood cancer journal, 13(1), 151. . ID: 37752130.
7.) Yang ZZ, Kim HJ, Wu H, Tang X, Yu Y, Krull J, Larson DP, Moore RM, Maurer MJ, Pavelko KD, Jalali S, Pritchett JC, Mudappathi R, Wang J, Villasboas JC, Mondello P, Novak AJ, Ansell SM (2023 Aug 18). T-cell phenotype including CD57(+) T follicular helper cells in the tumor microenvironment correlate with a poor outcome in follicular lymphoma. Blood cancer journal, 13(1), 124. . ID: 37591873.
8.) Doolittle ML, Saul D, Kaur J, Rowsey JL, Vos SJ, Pavelko KD, Farr JN, Monroe DG, Khosla S (2023 Jul 31). Multiparametric senescent cell phenotyping reveals targets of senolytic therapy in the aged murine skeleton. Nature communications, 14(1), 4587. . ID: 37524694.
9.) An Z, Hsu MA, Gicobi JK, Xu T, Harrington SM, Zhang H, Pavelko KD, Hirdler JB, Lohse CM, Nabavizadeh R, Pessoa RR, Sharma V, Thompson RH, Leibovich BC, Dong H, Lucien F (2023 Jun 15). A Novel PD-L1 Antibody Promotes Antitumor Function of Peripheral Cytotoxic Lymphocytes after Radical Nephrectomy in Patients with Renal Cell Carcinoma. Journal of immunology (Baltimore, Md. : 1950), 210(12), 2029-2037. . ID: 37163328.
10.) Dohnalkova E, Bayer RL, Guo Q, Bamidele AO, Kim Lee HS, Valenzuela-Pérez L, Krishnan A, Pavelko KD, Guisot NES, Bunyard P, Kim YB, Ibrahim SH, Gores GJ, Hirsova P (2023 Jun 1). Rho-associated protein kinase 1 inhibition in hepatocytes attenuates nonalcoholic steatohepatitis. Hepatology communications, 7(6), . . ID: 37267252.
11.) Farooqui N, Zaidi M, Vaughan L, McKee TD, Ahsan E, Pavelko KD, Villasboas JC, Markovic S, Taner T, Leung N, Dong H, Alexander MP, Herrmann SM (2023 Mar). Cytokines and Immune Cell Phenotype in Acute Kidney Injury Associated With Immune Checkpoint Inhibitors. Kidney international reports, 8(3), 628-641. . ID: 36938084.
12.) Doolittle ML, Saul D, Kaur J, Rowsey JL, Vos SJ, Pavelko KD, Farr JN, Monroe DG, Khosla S (2023 Jan 13). Multiparametric senescent cell phenotyping reveals CD24 osteolineage cells as targets of senolytic therapy in the aged murine skeleton. bioRxiv : the preprint server for biology, (), . . ID: 36711531.
13.) Barham W, Hsu M, Liu X, Harrington SM, Hirdler JB, Gicobi JK, Zhu X, Zeng H, Pavelko KD, Yan Y, Mansfield AS, Dong H (2023 Jan 1). A Novel Humanized PD-1/PD-L1 Mouse Model Permits Direct Comparison of Antitumor Immunity Generated by Food and Drug Administration-Approved PD-1 and PD-L1 Inhibitors. ImmunoHorizons, 7(1), 125-139. . ID: 36656137.
14.) Peterson SM, O (2023). Cross-sectional association between systemic metal concentrations and immune markers in patients with total joint arthroplasty. Frontiers in immunology, 14(), 1130209. . ID: 36993965.
15.) Zhang X, Pearsall VM, Carver CM, Atkinson EJ, Clarkson BDS, Grund EM, Baez-Faria M, Pavelko KD, Kachergus JM, White TA, Johnson RK, Malo CS, Gonzalez-Suarez AM, Ayasoufi K, Johnson KO, Tritz ZP, Fain CE, Khadka RH, Ogrodnik M, Jurk D, Zhu Y, Tchkonia T, Revzin A, Kirkland JL, Johnson AJ, Howe CL, Thompson EA, LeBrasseur NK, Schafer MJ (2022 Sep 27). Rejuvenation of the aged brain immune cell landscape in mice through p16-positive senescent cell clearance. Nature communications, 13(1), 5671. . ID: 36167854.
16.) Pritchett JC, Yang ZZ, Kim HJ, Villasboas JC, Tang X, Jalali S, Cerhan JR, Feldman AL, Ansell SM (2022 Jan). High-dimensional and single-cell transcriptome analysis of the tumor microenvironment in angioimmunoblastic T cell lymphoma (AITL). Leukemia, 36(1), 165-176. doi: 10.1038/s41375-021-01321-2. ID: 34230608.
17.) Alexander MP, Mangalaparthi KK, Madugundu AK, Moyer AM, Adam BA, Mengel M, Singh S, Herrmann SM, Rule AD, Cheek EH, Herrera Hernandez LP, Graham RP, Aleksandar D, Aubry MC, Roden AC, Hagen CE, Quinton RA, Bois MC, Lin PT, Maleszewski JJ, Cornell LD, Sethi S, Pavelko KD, Charlesworth J, Narasimhan R, Larsen CP, Rizza SA, Nasr SH, Grande JP, McKee TD, Badley AD, Pandey A, Taner T (2021 Oct). Acute Kidney Injury in Severe COVID-19 Has Similarities to Sepsis-Associated Kidney Injury: A Multi-Omics Study. Mayo Clinic proceedings, 96(10), 2561-2575. . ID: 34425963.
18.) Bartemes KR, Choby G, O (2021 Jul). Mass cytometry reveals unique subsets of T cells and lymphoid cells in nasal polyps from patients with chronic rhinosinusitis (CRS). Allergy, 76(7), 2222-2226. doi: 10.1111/all.14720. ID: 33370459.
19.) Yoo KH, Tang JJ, Rashid MA, Cho CH, Corujo-Ramirez A, Choi J, Bae MG, Brogren D, Hawse JR, Hou X, Weroha SJ, Oliveros A, Kirkeby LA, Baur JA, Jang MH (2021 Jul 1). Nicotinamide Mononucleotide Prevents Cisplatin-Induced Cognitive Impairments. Cancer research, 81(13), 3727-3737. . ID: 33771896.
20.) Lagnado A, Leslie J, Ruchaud-Sparagano MH, Victorelli S, Hirsova P, Ogrodnik M, Collins AL, Vizioli MG, Habiballa L, Saretzki G, Evans SA, Salmonowicz H, Hruby A, Geh D, Pavelko KD, Dolan D, Reeves HL, Grellscheid S, Wilson CH, Pandanaboyana S, Doolittle M, von Zglinicki T, Oakley F, Gallage S, Wilson CL, Birch J, Carroll B, Chapman J, Heikenwalder M, Neretti N, Khosla S, Masuda CA, Tchkonia T, Kirkland JL, Jurk D, Mann DA, Passos JF (2021 May 3). Neutrophils induce paracrine telomere dysfunction and senescence in ROS-dependent manner. The EMBO journal, 40(9), e106048. doi: 10.15252/embj.2020106048. ID: 33764576.
21.) Furuta K, Guo Q, Pavelko KD, Lee JH, Robertson KD, Nakao Y, Melek J, Shah VH, Hirsova P, Ibrahim SH (2021 Mar 15). Lipid-induced endothelial vascular cell adhesion molecule 1 promotes nonalcoholic steatohepatitis pathogenesis. The Journal of clinical investigation, 131(6), . . ID: 33476308.
22.) Mangaonkar AA, Reichard KK, Binder M, Coltro G, Lasho TL, Carr RM, Chiu A, Negron V, Hefazi M, Anagnostou T, Timm MM, Hiebert JW, Villasboas JC, Gonsalves WI, Gangat N, Shah M, Alkhateeb HB, Al-Kali A, Elliott MA, Begna KH, Wolanskyj-Spinner AP, Litzow MR, Hogan WJ, Ansell SM, Pardanani A, Tefferi A, Patnaik MM (2020 Nov 10). Bone marrow dendritic cell aggregates associate with systemic immune dysregulation in chronic myelomonocytic leukemia. Blood advances, 4(21), 5425-5430. . ID: 33152058.
23.) Dubey D, Wilson MR, Clarkson B, Giannini C, Gandhi M, Cheville J, Lennon VA, Eggers S, Devine MF, Mandel-Brehm C, Kryzer T, Hinson SR, Khazaie K, Hales C, Kattah J, Pavelko KD, Andrews P, Eaton JE, Jitprapaikulsan J, Mills JR, Flanagan EP, Zekeridou A, Leibovich B, Fryer J, Torre M, Kaufman C, Thoreson JB, Sagen J, Linnoila JJ, DeRisi JL, Howe CL, McKeon A, Pittock SJ (2020 Nov 1). Expanded Clinical Phenotype, Oncological Associations, and Immunopathologic Insights of Paraneoplastic Kelch-like Protein-11 Encephalitis. JAMA neurology, 77(11), 1420-1429. . ID: 32744608.
24.) Block MS, Dietz AB, Gustafson MP, Kalli KR, Erskine CL, Youssef B, Vijay GV, Allred JB, Pavelko KD, Strausbauch MA, Lin Y, Grudem ME, Jatoi A, Klampe CM, Wahner-Hendrickson AE, Weroha SJ, Glaser GE, Kumar A, Langstraat CL, Solseth ML, Deeds MC, Knutson KL, Cannon MJ (2020 Oct 14). Th17-inducing autologous dendritic cell vaccination promotes antigen-specific cellular and humoral immunity in ovarian cancer patients. Nature communications, 11(1), 5173. doi: 10.1038/s41467-020-18962-z. ID: 33057068.
25.) Dasgupta D, Nakao Y, Mauer AS, Thompson JM, Sehrawat TS, Liao CY, Krishnan A, Lucien F, Guo Q, Liu M, Xue F, Fukushima M, Katsumi T, Bansal A, Pandey MK, Maiers JL, DeGrado T, Ibrahim SH, Revzin A, Pavelko KD, Barry MA, Kaufman RJ, Malhi H (2020 Oct). IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis. Gastroenterology, 159(4), 1487-1503.e17. . ID: 32574624.
26.) Loeuillard E, Yang J, Buckarma E, Wang J, Liu Y, Conboy C, Pavelko KD, Li Y, O (2020 Oct 1). Targeting tumor-associated macrophages and granulocytic myeloid-derived suppressor cells augments PD-1 blockade in cholangiocarcinoma. The Journal of clinical investigation, 130(10), 5380-5396. . ID: 32663198.
27.) Kourelis TV, Jevremovic D, Jessen E, Dasari S, Villasboas JC, Dispenzieri A, Kumar S (2020 Jul). Mass cytometry identifies expansion of double positive and exhausted T cell subsets in the tumour microenvironment of patients with POEMS syndrome. British journal of haematology, 190(1), 79-83. doi: 10.1111/bjh.16522. ID: 32080834.
28.) Guo Q, Furuta K, Lucien F, Gutierrez Sanchez LH, Hirsova P, Krishnan A, Kabashima A, Pavelko KD, Madden B, Alhuwaish H, Gao Y, Revzin A, Ibrahim SH (2019 Dec). Integrin β<sub>1</sub>-enriched extracellular vesicles mediate monocyte adhesion and promote liver inflammation in murine NASH. Journal of hepatology, 71(6), 1193-1205. . ID: 31433301.
The Immune Monitoring Core at Mayo Clinic provides services to Mayo Clinic researchers seeking to study immunological phenomena. Services include:
Designing assays to identify and quantify immune cell subpopulations
Characterizing and measuring immune function
Monitoring immunological changes in response to both specific and nonspecific stimulation
Mass Cytometry (CyTOF, Helios)
Imaging Mass Cytometry (IMC, Hyperion)
Multiplex Immunofluorescence (Akoya Phenocycler-Fusion)
The core supports experimental systems to study human and mouse models by providing expertise and standardized assays to evaluate immune functions in a broad range of diseases and therapies in models as well as in healthy human subjects with variable demographics.
The Immune Monitoring Core provides services to all Mayo Clinic faculty and staff engaged in research. Selective services are available to investigators outside Mayo Clinic.
