University of Massachusetts Medical School
Contact Info:
Christina Baer
55 North Lake Ave
Worcester, MA 01655
https://www.umassmed.edu/scope/
Grants and Identifiers:
RRID: RRID:SCR_022721
Instrumentation:
(Equipment No Longer Active At this Facility)
Agilent Seahorse XFe96 Analyzer
Seahorse XFe96 Analyzer measures OCR and ECAR of live cells in 96 well plate format. [Product Link]
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(Equipment No Longer Active At this Facility)
Essen Incucyte Incucyte
IncuCyte ZOOM system is a live-cell imaging and analysis platform that enables automated quantification of cell behavior over time (from hours to weeks) by automatically gathering and analyzing images around the clock. The system provides insight into active biological processes in real-time which is not possible using single-point and end-point measurements. The system resides within the controlled environment of a standard cell incubator. All imaging is completely non-invasive and non-perturbing to cell health. The system can process multiple plates, flasks and dishes in parallel and does not depend on shuttling plates into and out of the incubator. [Product Link]
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(Equipment No Longer Active At this Facility)
Leica TCS SP5 Broadband Confocal Laser Scanning Microscope
Leica TCS SP5 is a broadband confocal microscope that provides the full range of scan speeds at the a high resolution. With its SP detection (five channels simultaneously) and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. The system is also the platform for the new Leica DM6000 CFS (Confocal Fixed Stage) for physiological and electrophysiological experiments and for the new super resolution Leica TCS STED confocal microscope. [Product Link]
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(Equipment No Longer Active At this Facility)
Nikon A1 Confocal Laser Microscope
Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]
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Services Provided:
Confocal/ Super Resolution Microscope
Data Analysis And Interpretation
Data Processing And Interpretation
Multiphoton/ Harmonic Generation Microscope
Relevant Publications:
Description:
Sanderson Center for Optical Experimentation (SCOPE) at UMass Chan Medical School offers the tools and expertise required to perform a wide variety of quantitative microscopy techniques including super resolution imaging, multi-photon intravital and live tissue imaging, confocal microscopy, and high-speed time-lapse imaging. The SCOPE offers a combination of commercial and custom-built microscopes, allowing users to address an extensive array of biological questions. In addition to user training and access to light microscopes, the SCOPE offers a wide range of imaging services including whole slide scanning, MERFISH spatial transcriptomics, NanoString GeoMx DSP services, and image analysis support. Wet lab facilities, including cell culture, are also available in this BSL2 facility. Imaging at BSL3 is available upon request. We offer remote imaging and data analysis sessions, including remote control of all instruments and workstations. All users are trained individually prior to use of the SCOPE equipment and workstations. Access for external users can be arranged following a consultation regarding project scope and expected outcomes with the Director.
