Howard Hughes Medical Institute
Contact Info:
Kathy Schaefer
19700 Helix Drive
Ashburn, VA 20147
https://www.janelia.org/support-team/flow-cytometry
Grants and Identifiers:
RRID: RRID:SCR_022690
Instrumentation:
(Equipment No Longer Active At this Facility)
BD Biosciences FACSAria IIu High Speed Cell Sorter
The High Speed Cell Sorter's primary function is to examine complex populations of cells and yield pure populations of cells. [Product Link]
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Beckman Coulter CytoFLEX Flow Cytometer
Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]
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Sony Biotechnology SH800S Cell Sorter
The benchtop SH800S cell sorter is a cell sorter that sorts a wide range of cell sizes for many applications using the 70-μm, 100-μm, and 130-μm microfluidic sorting chips. This chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective and easier to perform. System software supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. The SH800S is for non-clinical research use only and is a Class 1 laser product. The SH800S is flexible and can support a variety of applications for individual and core labs. For applications in which cross-contamination is a concern, optional e-beam sterilized consumables can be used to replace the sample line and sorting chip. An optional class A2 Level II biosafety cabinet provides protection for personnel and products. The biosafety cabinet was custom designed for the SH800S and tested by The Baker Company to meet international standards. The optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection. [Product Link]
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Other Equipment
BD Biosciences FACSAria SORP Invitrogen Bigfoot Spectral Sorter
Services Provided:
Relevant Publications:
Description:
The Flow Cytometry Shared Resource (FCSR) provides a range of instrumentation and expertise for cell sorting and analysis that can support experimental needs related to single cells, cell line production, biosensor development, and dye analysis, allowing researchers to develop new tools and answer questions about the nature and function of cells. The FCSR has expertise in flow cytometry experimental design, operation of instruments, distribution of raw data and sort reports, interpretation of results, and preparation of graphics for presentation and publication.
