Van Andel Institute
Rachael Sheridan
333 Bostwick Ave
Grand Rapids, MI 49503
https://flowcytometrycore.vai.org/
RRID: RRID:SCR_022685
(Equipment No Longer Active At this Facility)
Beckman Coulter Astrios Cell Sorter
MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions. The ergonomic design of Astrios makes it faster and easier to operate. All components requiring operator control are within easy reach. Beyond the optic expansion, novel software components enhance the instruments capabilities. The Touch Screen Control Panel facilitates... Read more [Product Link]
RRID:SCR_019615
Beckman Coulter CytoFLEX Flow Cytometer
Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]
RRID:SCR_019627
Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
RRID:SCR_019826
Fiber Optic Button Response System
Instrument that accurately gathers participant responses and verifies signals. The Celeritas Series response units are assembled using high-impact, chemical resistant, medical grade plastic. The response units include a tactile indicator to ensure correct finger placement during experiments and comfortably attach to the participant?s wrists. The units communicate button presses through fiber optic cabling which connects to a Fiber Optic Interface Console located in the control room through an available wave guide. The interface console provides real-time feedback of participant responses via LED indicators and includes a set of switches which can be used to make responses for the participant as needed. [Product Link]
RRID:SCR_009577
BD FACS Symphony S6
Cell sorter
Thermo Fisher Attune CytPix
Imaging Cytometer
BD FACSymphony S6
Abaxis HM5
Vestscan 2
Cell Sorting
Consultation
Flow Cytometric Analysis
Flow Cytometry Data Analysis
High Dimensional Flow Cytometry Analysis
Multi-color Flow Cytometry
Phenotyping
Protocol Development
RNA Flow Cytometry
Staining
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2.) Ide AD, Carpenter KA, Elaswad MT, Opria K, Marcellin K, Gilliland C, Grainger S (2026 Jan). Secreted frizzled-related protein 1a regulates hematopoietic development in a dose-dependent manner. Developmental biology, 529(), 1-12. doi: 10.1016/j.ydbio.2025.09.019. PMID: 41016526.
3.) Oswald BM, DeCamp LM, Longo J, Dahabieh MS, Bunda N, Johnson BK, Watson MJ, Ma S, Preston SE, Sheldon RD, Vincent MP, Ellis AE, Soper-Hopper MT, Isaguirre C, Kamarudin D, Shen H, Williams KS, Crawford PA, Kaech S, Jang HJ, Lien EC, Krawczyk CM, Jones RG (2025 Dec 9). Dietary restriction reprograms CD8+ T cell fate to enhance anti-tumour immunity and immunotherapy responses Nature Metabolism, 7(12), 2489-2509. . PMCID: 12727518.
4.) Norden PR, Wedan RJ, Preston SE, Canfield M, Graber N, Longenecker JZ, Pentecost OA, McLaughlin E, Hart ML, Nowinski SM (2025 Oct 6). Mitochondrial phosphopantetheinylation is required for oxidative metabolism Metabolism: clinical and experimental, 174(), 156413. . PMCID: 12671336.
5.) Buteyn NJ, LaMantia SJ, Burke CG, Sartori VJ, Deering-Gardner E, DeBruine ZJ, Kamarudin D, Chandler DP, Monovich AC, Perez MW, Yi JS, Ries RE, Alonzo TA, Ryan RJ, Meshinchi S, Triche TJ Jr (2025 Sep 18). EZH2-driven immune evasion at disease presentation defines a targetable high-risk subset of acute leukemia exemplified by t(16;21) FUS::ERG AML bioRxiv, (), 2024.05.14.594150. . PMCID: 11118270.
6.) Ensing J, Ide AD, Gilliland C, Tsurho V, Rudisel E, Caza I, Stratman AN, Lanning NJ, Grainger S (2025 Sep 15). The E3 ubiquitin ligase Trip12 semi-selectively attenuates Wnt signaling iScience, 28(10), 113575. . PMCID: 12519312.
7.) Kaluba FC, Rogers TJ, Jeong YJ, House R‘, Waldhart A, Sokol KH, Daniels SR, Lee CJ, Longo J, Johnson A, Sartori VJ, Sheldon RD, Jones RG, Lien EC (2025 Sep 8). An alternative route for β-hydroxybutyrate metabolism supports cytosolic acetyl-CoA synthesis in cancer cells Nature Metabolism, 7(10), 2033-2044. . PMCID: 12552118.
8.) Spix NJ, Habib WA, Zhang Z, Eugster E, Milliron HY, Sokol D, Lee K, Nolte PA, Endicott JL, Krzyzanowski KF, Hinoue T, Morrison J, Johnson BK, Zhou W, Shen H, Laird PW (2025 Jul 8). High-coverage allele-resolved single-cell DNA methylation profiling reveals cell lineage, X-inactivation state, and replication dynamics Nature Communications, 16(), 6273. . PMCID: 12234882.
9.) Dahabieh MS, DeCamp LM, Oswald BM, Kitchen-Goosen SM, Fu Z, Vos M, Compton SE, Longo J, Foy NM, Williams KS, Ellis AE, Johnson A, Sodiya I, Vincent M, Lee H, Yao C, Wu T, Sheldon RD, Krawczyk CM, Jones RG (2025 Jun 27). The prostacyclin receptor PTGIR is a NRF2-dependent regulator of CD8+ T cell exhaustion Nature Immunology, 26(7), 1139-1151. . PMCID: 12208871.
10.) Zhang C, Zhu J, Lin H, Zhang Z, Kang B, Li F, Shan Y, Zhang Y, Xing Q, Gu J, Hu X, Cui Y, Huang J, Zhou T, Mai Y, Chen Q, Mao R, Li P, Pan G (2025 Apr 14). HBO1 determines epithelial-mesenchymal transition and promotes immunotherapy resistance in ovarian cancer cells Cellular Oncology (Dordrecht, Netherlands), 48(4), 943-959. . PMCID: 12238117.
11.) Sokol KH, Lee CJ, Rogers TJ, Waldhart A, Ellis AE, Madireddy S, Daniels SR, House RRJ, Ye X, Olesnavich M, Johnson A, Furness BR, Sheldon RD, Lien EC (2025 Mar 20). Lipid availability influences ferroptosis sensitivity in cancer cells by regulating polyunsaturated fatty acid trafficking. Cell chemical biology, 32(3), 408-422.e6. . PMCID: 11928283.
12.) Xue Z, Xuan H, Lau K, Su Y, Wegener M, Li K, Turner L, Adams M, Shi X, Wen H (2025 Mar 14). Expression of ENL YEATS domain tumor mutations in nephrogenic or stromal lineage impairs kidney development Nature Communications, 16(), 2531. . PMCID: 11909213.
13.) Pierce SE, van der Schans EJC, Goralski TM, Ensink E, Li P, Henderson MX, Coetzee GA (2025). Gene expression asymmetry in Parkinson's disease: variation of CCT gene expression is correlated with hemisphere specific severity. Frontiers in molecular neuroscience, 18(), 1743557. . PMCID: 12835222.
14.) Jang HJ, Urrutia G, Orskov AD, Kim HJ, Nelson SA, Nguyen AV, Lee H, Burgos RS, Johnson BK, Wegener M, Becker K, Adams M, Sheridan R, Ramjan ZH, Givan SA, Zebley CC, Youngblood BA, Issa JJ, Topper MJ, Baylin SB, Baer MR, Triche TJ, O'Connell CL, Gronbaek K, Jones PA (2024 Nov 11). Bone marrow microenvironment signatures associate with patient survival after guadecitabine and atezolizumab therapy in HMA-resistant MDS. bioRxiv : the preprint server for biology, (), . . PMID: 39605731.
15.) Norden PR, Wedan RJ, Longenecker JZ, Preston SEJ, Graber N, Pentecost OA, Canfield M, McLaughlin E, Nowinski SM (2024 Nov 4). Mitochondrial Phosphopantetheinylation is Required for Oxidative Function. bioRxiv : the preprint server for biology, (), . . PMID: 38766035.
16.) Kaluba FC, Rogers TJ, Jeong YJ, Waldhart A, Sokol KH, Lee CJ, Daniels SR, Longo J, Johnson A, Sheldon RD, Jones RG, Lien EC (2024 Nov 3). An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells bioRxiv, (), 2024.10.31.621317. . PMCID: 11565918.
17.) Longo J, DeCamp LM, Oswald BM, Teis R, Reyes-Oliveras A, Dahabieh MS, Ellis AE, Vincent MP, Damico H, Gallik KL, Compton SE, Capan CD, Williams KS, Esquibel CR, Madaj ZB, Lee H, Roy DG, Krawczyk CM, Haab BB, Sheldon RD, Jones RG (2024 Oct 14). Glucose-dependent glycosphingolipid biosynthesis fuels CD8(+) T cell function and tumor control. bioRxiv : the preprint server for biology, (), . . PMID: 39464161.
18.) Xue Z, Qin L, Xuan H, Luo K, Huang M, Xie L, Su Y, Xu L, Harsh J, Dale B, Shi X, Chen X, Kaniskan HÜ, Jin J, Wen H (2024 Aug 28). A potent and selective ENL degrader suppresses oncogenic gene expression and leukemia progression Science Advances, 10(35), eado1432. . PMCID: 11352836.
19.) Guak H, Weiland M, Ark AV, Zhai L, Lau K, Corrado M, Davidson P, Asiedu E, Mabvakure B, Compton S, DeCamp L, Scullion CA, Jones RG, Nowinski SM, Krawczyk CM (2024 Jul 24). Transcriptional programming mediated by the histone demethylase KDM5C regulates dendritic cell population heterogeneity and function Cell reports, 43(8), 114506. . PMCID: 11416765.
20.) Chomiak AA, Tiedemann RL, Liu Y, Kong X, Cui Y, Wiseman AK, Thurlow KE, Cornett EM, Topper MJ, Baylin SB, Rothbart SB (2024 Mar 27). Select EZH2 inhibitors enhance viral mimicry effects of DNMT inhibition through a mechanism involving NFAT:AP-1 signaling Science Advances, 10(13), eadk4423. . PMCID: 10971413.
21.) Booms A, Pierce SE, van der Schans EJ, Coetzee GA (2024 Jan 17). Parkinson’s disease risk enhancers in microglia iScience, 27(2), 108921. . PMCID: 10845915.
The VAI Flow Cytometry Core provides cell analysis and sorting services. We have a Cytek Aurora, Beckman Coulter CytoFLEX S, Attune CytPix, SONY MA900, and a BD FACSSymphony S6
