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VAI Flow Cytometry Core Facility

Van Andel Institute

Contact Info:

Rachael Sheridan

333 Bostwick Ave

Grand Rapids, MI 49503

https://flowcytometrycore.vai.org/

Grants and Identifiers:

RRID: RRID:SCR_022685

Instrumentation:

(Equipment No Longer Active At this Facility)

Beckman Coulter Astrios Cell Sorter

MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions. The ergonomic design of Astrios makes it faster and easier to operate. All components requiring operator control are within easy reach. Beyond the optic expansion, novel software components enhance the instruments capabilities. The Touch Screen Control Panel facilitates... Read more [Product Link]

RRID:SCR_019615

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Beckman Coulter CytoFLEX Flow Cytometer

Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]

RRID:SCR_019627

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Cytek Aurora Spectral Analyzer

Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]

RRID:SCR_019826

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Sony MA900 Multi-Application Cell Sorter

MA900 system include patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that simplifies operation to make sorting less subjective and improve reliability. Provides up to 14 parameters from 4 lasers with possibility to choose from 4 excitation lasers—488 nm, 405 nm, 561 nm, and 638 nm—on two beam spots. Free-form PMTs enable detection of fluorescence signals from each beam spot, allowing detection of up to 12 fluorescence parameters and two scatter parameters. [Product Link]

RRID:SCR_026300

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BD FACS Symphony S6

Cell sorter

Thermo Fisher Attune CytPix

Imaging Cytometer

Other Equipment

BD FACSymphony S6 Abaxis HM5 Vestscan 2

Services Provided:

Cell Sorting

Consultation

Flow Cytometric Analysis

Flow Cytometry Data Analysis

High Dimensional Flow Cytometry Analysis

Multi-color Flow Cytometry

Phenotyping

Protocol Development

RNA Flow Cytometry

Staining

Relevant Publications:

1.) Buteyn NJ, LaMantia SJ, Burke CG, Sartori VJ, Deering-Gardner E, DeBruine ZJ, Kamarudin D, Chandler DP, Monovich AC, Perez MW, Yi JS, Ries RE, Alonzo TA, Ryan RJ, Meshinchi S, Triche TJ Jr (2025 Sep 18). EZH2-driven immune evasion at disease presentation defines a targetable high-risk subset of acute leukemia exemplified by t(16;21) FUS::ERG AML bioRxiv, (), 2024.05.14.594150. . PMCID: 11118270.


2.) Ensing J, Ide AD, Gilliland C, Tsurho V, Rudisel E, Caza I, Stratman AN, Lanning NJ, Grainger S (2025 Sep 15). The E3 ubiquitin ligase Trip12 semi-selectively attenuates Wnt signaling iScience, 28(10), 113575. . PMCID: 12519312.


3.) Kaluba FC, Rogers TJ, Jeong YJ, House R‘, Waldhart A, Sokol KH, Daniels SR, Lee CJ, Longo J, Johnson A, Sartori VJ, Sheldon RD, Jones RG, Lien EC (2025 Sep 8). An alternative route for β-hydroxybutyrate metabolism supports cytosolic acetyl-CoA synthesis in cancer cells Nature Metabolism, 7(10), 2033-2044. . PMCID: 12552118.


4.) Spix NJ, Habib WA, Zhang Z, Eugster E, Milliron HY, Sokol D, Lee K, Nolte PA, Endicott JL, Krzyzanowski KF, Hinoue T, Morrison J, Johnson BK, Zhou W, Shen H, Laird PW (2025 Jul 8). High-coverage allele-resolved single-cell DNA methylation profiling reveals cell lineage, X-inactivation state, and replication dynamics Nature Communications, 16(), 6273. . PMCID: 12234882.


5.) Dahabieh MS, DeCamp LM, Oswald BM, Kitchen-Goosen SM, Fu Z, Vos M, Compton SE, Longo J, Foy NM, Williams KS, Ellis AE, Johnson A, Sodiya I, Vincent M, Lee H, Yao C, Wu T, Sheldon RD, Krawczyk CM, Jones RG (2025 Jun 27). The prostacyclin receptor PTGIR is a NRF2-dependent regulator of CD8+ T cell exhaustion Nature Immunology, 26(7), 1139-1151. . PMCID: 12208871.


6.) Zhang C, Zhu J, Lin H, Zhang Z, Kang B, Li F, Shan Y, Zhang Y, Xing Q, Gu J, Hu X, Cui Y, Huang J, Zhou T, Mai Y, Chen Q, Mao R, Li P, Pan G (2025 Apr 14). HBO1 determines epithelial-mesenchymal transition and promotes immunotherapy resistance in ovarian cancer cells Cellular Oncology (Dordrecht, Netherlands), 48(4), 943-959. . PMCID: 12238117.


7.) Xue Z, Xuan H, Lau K, Su Y, Wegener M, Li K, Turner L, Adams M, Shi X, Wen H (2025 Mar 14). Expression of ENL YEATS domain tumor mutations in nephrogenic or stromal lineage impairs kidney development Nature Communications, 16(), 2531. . PMCID: 11909213.


8.) Norden PR, Wedan RJ, Longenecker JZ, Preston SEJ, Graber N, Pentecost OA, Canfield M, McLaughlin E, Nowinski SM (2024 Nov 4). Mitochondrial Phosphopantetheinylation is Required for Oxidative Function. bioRxiv : the preprint server for biology, (), . . PMID: 38766035.


9.) Kaluba FC, Rogers TJ, Jeong YJ, Waldhart A, Sokol KH, Lee CJ, Daniels SR, Longo J, Johnson A, Sheldon RD, Jones RG, Lien EC (2024 Nov 3). An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells bioRxiv, (), 2024.10.31.621317. . PMCID: 11565918.


10.) Longo J, DeCamp LM, Oswald BM, Teis R, Reyes-Oliveras A, Dahabieh MS, Ellis AE, Vincent MP, Damico H, Gallik KL, Compton SE, Capan CD, Williams KS, Esquibel CR, Madaj ZB, Lee H, Roy DG, Krawczyk CM, Haab BB, Sheldon RD, Jones RG (2024 Oct 14). Glucose-dependent glycosphingolipid biosynthesis fuels CD8(+) T cell function and tumor control. bioRxiv : the preprint server for biology, (), . . PMID: 39464161.


11.) Xue Z, Qin L, Xuan H, Luo K, Huang M, Xie L, Su Y, Xu L, Harsh J, Dale B, Shi X, Chen X, Kaniskan HÜ, Jin J, Wen H (2024 Aug 28). A potent and selective ENL degrader suppresses oncogenic gene expression and leukemia progression Science Advances, 10(35), eado1432. . PMCID: 11352836.


12.) Guak H, Weiland M, Ark AV, Zhai L, Lau K, Corrado M, Davidson P, Asiedu E, Mabvakure B, Compton S, DeCamp L, Scullion CA, Jones RG, Nowinski SM, Krawczyk CM (2024 Jul 24). Transcriptional programming mediated by the histone demethylase KDM5C regulates dendritic cell population heterogeneity and function Cell reports, 43(8), 114506. . PMCID: 11416765.


13.) Chomiak AA, Tiedemann RL, Liu Y, Kong X, Cui Y, Wiseman AK, Thurlow KE, Cornett EM, Topper MJ, Baylin SB, Rothbart SB (2024 Mar 27). Select EZH2 inhibitors enhance viral mimicry effects of DNMT inhibition through a mechanism involving NFAT:AP-1 signaling Science Advances, 10(13), eadk4423. . PMCID: 10971413.


14.) Booms A, Pierce SE, van der Schans EJ, Coetzee GA (2024 Jan 17). Parkinson’s disease risk enhancers in microglia iScience, 27(2), 108921. . PMCID: 10845915.


Description:

The VAI Flow Cytometry Core provides cell analysis and sorting services. We have a Cytek Aurora, Beckman Coulter CytoFLEX S, Attune CytPix, SONY MA900, and a BD FACSSymphony S6