Northeastern University

Contact Info:

Guoxin Rong

805 Columbus Ave, Basement 080

Boston, MA 02115

https://cils.northeastern.edu/facilities

Grants and Identifiers:

RRID: RRID:SCR_022681

Instrumentation:

Other Equipment

Zeiss LSM 800 Confocal Laser Scanning Microscope Zeiss LSM 880 NLO with Airyscan Multi-Photon Confocal Laser Scanning Microscope ZEISS Axio Zoom.V16 for Biology Fluorescence Stereo Zoom Microscope Bruker 3T Biospec Magnetic Resonance Imaging Beckman Coulter CytoFLEX S Flow Cytometer Beckman Coulter CytoFLEX SRT Cell Sorter VisualSonics Vevo F2 Ultrasound NanoTemper Monolith NT Microscale Thermophoresis

Services Provided:

3D & 4D Image Processing Stations

3D Visualization

Cell Culture

Cell Imaging

Cell Sorting

Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI)

Confocal Microscope With FCS/FLIM Capabilities

Confocal Microscopy

Confocal/ Super Resolution Microscope

Consultation

FACS Cell Sorting

Fluorescence Microscope With Optical Sectioning Capability

Functional Magnetic Resonance Imaging (fMRI)

Image Processing And Analysis

In-vivo Imaging

Light-sheet Microscopy

Live Cell Imaging

Magnetic Resonance Imaging (MRI)

MicroScale Thermophoresis (MST)

Microscopy

Multi-color Flow Cytometry

Multiphoton Microscopy

Multiphoton/ Harmonic Generation Microscope

Optical Imaging

Super-resolution Microscopy

Training

Ultrasonic Imaging

Widefield Microscopy

Relevant Publications:

1.) Raustad N, Dai Y, Iinishi A, Mohapatra A, Soo MW, Hay E, Hernandez GM, Geisinger E (2025 Feb 8). A phosphorylation signal activates genome-wide transcriptional control by BfmR, the global regulator of Acinetobacter resistance and virulence Nucleic Acids Research, 53(4), gkaf063. . PMCID: 11806355.

2.) Lynch WB, Miracle SA, Goldstein SI, Beierle JA, Bhandari R, Gerhardt ET, Farnan A, Nguyen BM, Wingfield KK, Kazerani I, Saavedra GA, Averin O, Baskin BM, Ferris MT, Reilly CA, Emili A, Bryant CD (2024 Sep 1). Validation studies and multi-omics analysis of Zhx2 as a candidate quantitative trait gene underlying brain oxycodone metabolite (oxymorphone) levels and behavior bioRxiv, (), 2024.08.30.610534. . PMCID: 11383981.

3.) Perera-Gonzalez M, MacAskill CJ, Clark HA, Flask CA (2024 Jul 29). Fast quantitative MRI: Spiral Acquisition Matching-Based Algorithm (SAMBA) for Robust T1 and T2 Mapping Journal of magnetic resonance open, 20(), 100157. . PMCID: 11423800.

4.) Okorafor CC, Shastri S, Wen K, Ebong EE (2024 Jul 15). Mechanisms of triple-negative breast cancer extravasation: Impact of the physical environment and endothelial glycocalyx. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 38(13), e23785. doi: 10.1096/fj.202400380R. PMID: 38949120.

5.) Timilsina S, Saad MA, Lang RT, Hasan T, Spring BQ (2024 May 10). Methods for assessing and removing non‐specific photoimmunotherapy damage in patient‐derived tumor cell culture models Photochemistry and Photobiology, 101(1), 4-20. . PMCID: 11550265.

6.) Ma KY, Perera-Gonzalez M, Langlois NI, Alzubi OM, Guimond JD, Flask CA, Clark HA (2024 Apr 18). pH-responsive i-motif-conjugated nanoparticles for MRI analysis. Sensors & diagnostics, 3(4), 623-630. . PMID: 38646186.

7.) Bertucci T, Kakarla S, Winkelman MA, Lane K, Stevens K, Lotz S, Grath A, James D, Temple S, Dai G (2023 Aug 8). Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells APL Bioengineering, 7(3), 036107. . PMCID: 10411996.

Description:

Institute for Chemical Imaging of Living Systems (CILS) at Northeastern includes the latest advanced imaging instruments on the market. CILS has three core facilities: Microscopy Core (confocal/multi-photon/lightsheet microscopes), Flow Cytometry Core (cell analyzer/sorter) and Preclinical Imaging Core (3T small animal MRI and ultrasound).