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Bio-Imaging and Analysis Facility

Cincinnati Children's Hospital

Contact Info:

Matt Kofron

3333 Burnet Avenue

Cincinnati, OH 45229-3026

https://www.cincinnatichildrens.org/research/cores/confocal-imaging

Grants and Identifiers:

RRID: RRID:SCR_022628

Instrumentation:

Services Provided:

Relevant Publications:

1.) Peters AL, DePasquale EAK, Begum G, Roskin KM, Woodle ES, Hildeman DA (2024 Feb 29). Defining the T cell transcriptional landscape in pediatric liver transplant rejection at single cell resolution. bioRxiv : the preprint server for biology, (), . . PMID: 38464256.


2.) Carpenter J, Yarlagadda S, VandenHeuvel KA, Ding L, Schuh MP (2024 Feb). Human Nephrogenesis can Persist Beyond 40 Postnatal Days in Preterm Infants. Kidney international reports, 9(2), 436-450. . PMID: 38344733.


3.) He H, Bell SM, Davis AK, Zhao S, Sridharan A, Na CL, Guo M, Xu Y, Snowball J, Swarr DT, Zacharias WJ, Whitsett JA (2023 Dec 20). PRDM3/16 Regulate Chromatin Accessibility Required for NKX2-1 Mediated Alveolar Epithelial Differentiation and Function bioRxiv, (), 2023.12.20.570481. . PMCID: 10769259.


4.) He H, Bell SM, Davis AK, Zhao S, Sridharan A, Na CL, Guo M, Xu Y, Snowball J, Swarr DT, Zacharias WJ, Whitsett JA (2023 Dec 20). PRDM3/16 Regulate Chromatin Accessibility Required for NKX2-1 Mediated Alveolar Epithelial Differentiation and Function. bioRxiv : the preprint server for biology, (), . . PMID: 38187557.


5.) Pode-Shakked N, Slack M, Sundaram N, Schreiber R, McCracken KW, Dekel B, Helmrath M, Kopan R (2023 Dec 9). RAAS-deficient organoids indicate delayed angiogenesis as a possible cause for autosomal recessive renal tubular dysgenesis. Nature communications, 14(1), 8159. . PMID: 38071212.


6.) Parkins EV, Brager DH, Rymer JK, Burwinkel JM, Rojas D, Tiwari D, Hu YC, Gross C (2023 Dec 8). Mir324 knockout regulates the structure of dendritic spines and impairs hippocampal long-term potentiation. Scientific reports, 13(1), 21919. . PMID: 38082035.


7.) LaSarge CL, McCoy C, Namboodiri DV, Hartings JA, Danzer SC, Batie MR, Skoch J (2023 Dec). Spatial and Temporal Comparisons of Calcium Channel and Intrinsic Signal Imaging During in Vivo Cortical Spreading Depolarizations in Healthy and Hypoxic Brains. Neurocritical care, 39(3), 655-668. doi: 10.1007/s12028-022-01660-7. PMID: 36539593.


8.) Edwards NA, Kashyap A, Warren A, Agricola ZN, Kenny AP, Shen Y, Chung WK, Zorn AM (2023 Nov 21). Disrupted endosomal trafficking of the Vangl-Celsr polarity complex underlies congenital anomalies in trachea-esophageal morphogenesis. bioRxiv : the preprint server for biology, (), . . PMID: 37873300.


9.) Reza HA, Farooqui Z, Reza AA, Conroy C, Iwasawa K, Ogura Y, Okita K, Osafune K, Takebe T (2023 Nov 14). Synthetic augmentation of bilirubin metabolism in human pluripotent stem cell-derived liver organoids. Stem cell reports, 18(11), 2071-2083. . PMID: 37832542.


10.) Carpenter J, Yarlagadda S, VandenHeuvel KA, Ding L, Schuh MP (2023 Nov 4). Human Nephrogenesis can Persist Beyond 40 Postnatal Days in Preterm Infants Kidney International Reports, 9(2), 436-450. . PMCID: 10851065.


11.) Múnera JO, Kechele DO, Bouffi C, Qu N, Jing R, Maity P, Enriquez JR, Han L, Campbell I, Mahe MM, McCauley HA, Zhang X, Sundaram N, Hudson JR, Zarsozo-Lacoste A, Pradhan S, Tominaga K, Sanchez JG, Weiss AA, Chatuvedi P, Spence JR, Hachimi M, North T, Daley GQ, Mayhew CN, Hu YC, Takebe T, Helmrath MA, Wells JM (2023 Nov 2). Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon Cell stem cell, 30(11), 1434-1451.e9. . PMCID: 10913028.


12.) Múnera JO, Kechele DO, Bouffi C, Qu N, Jing R, Maity P, Enriquez JR, Han L, Campbell I, Mahe MM, McCauley HA, Zhang X, Sundaram N, Hudson JR, Zarsozo-Lacoste A, Pradhan S, Tominaga K, Sanchez JG, Weiss AA, Chatuvedi P, Spence JR, Hachimi M, North T, Daley GQ, Mayhew CN, Hu YC, Takebe T, Helmrath MA, Wells JM (2023 Nov 2). Development of functional resident macrophages in human pluripotent stem cell-derived colonic organoids and human fetal colon. Cell stem cell, 30(11), 1434-1451.e9. . PMID: 37922878.


13.) Parkins EV, Burwinkel JM, Ranatunga R, Yaser S, Hu YC, Tiwari D, Gross C (2023 Oct). Age-Dependent Regulation of Dendritic Spine Density and Protein Expression in Mir324 KO Mice. Journal of molecular neuroscience : MN, 73(9), 818-830. . PMID: 37773316.


14.) Mikedis MM, Liu B, de Rooij DG, Page DC (2023 Sep 22). Post-transcriptional repression of mRNA enhances competence to transit from mitosis to meiosis in mouse spermatogenic cells bioRxiv, (), 2023.09.20.557439. . PMCID: 10541148.


15.) Mikedis MM, Liu B, de Rooij DG, Page DC (2023 Sep 22). Post-transcriptional repression of mRNA enhances competence to transit from mitosis to meiosis in mouse spermatogenic cells. bioRxiv : the preprint server for biology, (), . . PMID: 37781613.


16.) Parkins EV, Burwinkel JM, Ranatunga R, Yaser S, Hu YC, Tiwari D, Gross C (2023 Aug 7). Age-dependent regulation of dendritic spine density and protein expression in Mir324 KO mice. Research square, (), . . PMID: 37609225.


17.) Witcher PC, Sun C, Millay DP (2023 May 1). Expression of Myomaker and Myomerger in myofibers causes muscle pathology Skeletal Muscle, 13(), 8. . PMCID: 10150476.


18.) Witcher PC, Sun C, Millay DP (2023 May 1). Expression of Myomaker and Myomerger in myofibers causes muscle pathology. Skeletal muscle, 13(1), 8. . PMID: 37127758.


19.) Doll JR, Moreno-Fernandez ME, Stankiewicz TE, Wayland JL, Wilburn A, Weinhaus B, Chougnet CA, Giordano D, Cappelletti M, Presicce P, Kallapur SG, Salomonis N, Tilburgs T, Divanovic S (2023 Apr 25). BAFF and APRIL counterregulate susceptibility to inflammation-induced preterm birth. Cell reports, 42(4), 112352. . PMID: 37027297.


Description:

The Bio-Imaging and Analysis Facility at Cincinnati Children\'s Hospital Medical Center (CCHMC) provides access to and training on state-of-the-art optical microscopes and associated equipment. The facility and expertise are available to investigators at CCHMC and the University of Cincinnati College of Medicine. The BIAF is a Nikon Center of Excellence. This agreement combines the biological expertise of CCHMC with Nikon’s technical proficiency to further new developments in biomedical sciences to improve child health.