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TAMU-AgriGenomics Laboratory

Texas A&M University

Contact Info:

Robert Vaughn

954 Agronomy Rd

Rm 116-117

College Station, TX 77840

http://agl.tamu.edu/

Grants and Identifiers:

RRID: RRID:SCR_022533

Instrumentation:

BD Biosciences C6 Accuri Flow Cytometer

BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. With its compact 11 x 14.75 x 16.5-inch footprint, light weight of 30 lb, and operational simplicity, the BD Accuri C6 Plus supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. [Product Link]

RRID:SCR_019591

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GenoGrinder High Throughput Sample Homogenizer (SPEX)

Geno/Grinder is an automated high-throughput plant & animal tissue homogenizer and cell lyser. It is equipped with an adjustable clamp that accommodates a full range of sample vials from 2mL to 50mL centrifuge tubes or up to six deep-well titer plates. It is specifically designed for rapid cell disruption, cell lysis and tissue homogenization through bead beating. This enables fast and efficient extraction of nucleic acids, proteins and other molecules. The new model Geno/Grinder incorporates a password protected, touch screen control panel, enabling the user to program run time, rate, cycles and pause time. A full range of Kryotech accessories are available to preserve temperature sensitive samples such as proteins and RNA. The Geno/Grinder is also recognized as an excellent automatic axial extractor. It is the best tool for the QuEChERS method used for extracting pesticide residues and other organic compounds. The Geno/Grinder enables increased throughput, improved extraction efficiency and reproducibility over traditional sample preparation methods. A full range of pre-loaded sample vials for different sample types is also available for fast and set up. [Product Link]

RRID:SCR_019966

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Other Equipment

PHERAstar Plus Microplate Reader Denovix DS-11 spectrophotometer/fluorometer Fluidigm BioMark HD IFC controler MX IFC controler HX Hydrocycler: high capacity thermocycler Bioer GenePro 96-well thermocycler ABI Veriti 384-well thermocycler MP Scientific FastPrep-24 homogenizer Apricot Designs i-Pipette 96 Nuaire refrigerated centrifuge Labnet Accuseal semi-automated plate sealer Thermofisher Barnstead NanopureĀ® water filtration system Labconco FreeZoneĀ® 6L freeze dryer BeadBlaster 24 tube homogenizer Lab Doctor Plate Centrifuge Eppendorf 5810R Plate Centrifuge Eppendorf 5417C Tube Centrifuge

Services Provided:

Copy Number Variation (CNV)

Data Analysis

DNA Analysis

DNA Isolation

Experimental Design

Genotyping

High-throughput Screening

Nucleic Acid Extraction

Plate Reader

Protocol Development

Reagents

RNA analysis

RNA Integrity

RNA Isolation

Relevant Publications:

Description:

The primary goal of the AgriGenomics Lab (AGL) is to assist graduate students, staff and faculty launch, economize and expedite their research, especially when it involves DNA extraction and quality checks, targeted genotyping and DNA content analysis. We also will, as needed, facilitate usage of complementary cores and assist with research planning and data analysis. AGL members have free lab access and reduced costs for services as well as one-on-one training and advising with AGL staff. TAMU system users can purchase services at a cost-effective rates. This service is especially useful for investigators interested in exploring new techniques, and obtaining pilot data sets for external funding proposals. Non-system members are also welcome to set up projects using AGL facilities.