Penn State College of Medicine

Contact Info:

Han Chen

500 University Drive, P.O.Box 850

Room C1730

Hershey, PA 17033

https://research.med.psu.edu/core-facilities/light-microscopy/

Grants and Identifiers:

RRID: RRID:SCR_022526

Instrumentation:

Other Equipment

Leica SP8 FCS/ FLIM Light Sheet Fluorescent Microscope (LSFM) Leica SP8 STED 3X Inverted Confocal Nikon A1 MP+ Upright Multiphoton DeltaVisionElite Deconvolution Inverted Image processing workstation

Services Provided:

Confocal Microscope With FCS/FLIM Capabilities

Confocal Microscopy

Confocal/ Super Resolution Microscope

Microscopy

Multiphoton Microscopy

Super-resolution Microscopy

Relevant Publications:

1.) Pale LM, Khatib JB, Nicolae CM, Moldovan GL (2024 Apr 20). CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps bioRxiv, (), 2024.04.17.589988. . PMCID: 11042333.

2.) Ye Y, Liang X, Wang G, Bewley MC, Hamamoto K, Liu X, Flanagan JM, Wang HG, Takahashi Y, Tian F (2024 Mar 15). Identification of membrane curvature sensing motifs essential for VPS37A phagophore recruitment and autophagosome closure Communications Biology, 7(), 334. . PMCID: 10942982.

3.) Lambert GS, Rice BL, Kaddis Maldonado RJ, Chang J, Parent LJ (2024 Jan 18). Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome bioRxiv, (), 2024.01.18.575255. . PMCID: 10827203.

4.) Kazzaz SA, Shaikh KA, White J, Zhou Q, Powell WH, Harhaj EW (2024 Jan). Phosphorylation of aryl hydrocarbon receptor interacting protein by TBK1 negatively regulates IRF7 and the type I interferon response. The Journal of biological chemistry, 300(1), 105525. . PMID: 38043800.

5.) Saha K, Subramenium Ganapathy A, Wang A, Arumugam P, Michael Morris N, Harris L, Yochum G, Koltun W, Perdew GH, Nighot M, Ma T, Nighot P (2023 Dec). Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation. Mucosal immunology, 16(6), 826-842. . PMID: 37716509.

6.) Chang J, Parent LJ (2023 Nov 22). HIV-1 Gag co-localizes with euchromatin histone marks at the nuclear periphery Journal of Virology, 97(12), e01179-23. . PMCID: 10734548.

7.) Hattori T, Fundora KA, Hamamoto K, Opozda DM, Liang X, Liu X, Zhang J, Uzun Y, Takahashi Y, Wang HG (2023 Sep 21). ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosis Autophagy, 20(2), 349-364. . PMCID: 10813646.

8.) Pandya Shesh B, Slagle-Webb B, Shenoy G, Khristov V, Zacharia BE, Connor JR (2023 Sep). Uptake of H-ferritin by Glioblastoma stem cells and its impact on their invasion capacity. Journal of cancer research and clinical oncology, 149(12), 9691-9703. doi: 10.1007/s00432-023-04864-2. PMID: 37237166.

9.) Kaddis Maldonado R, Lambert GS, Rice BL, Sudol M, Flanagan JM, Parent LJ (2023 Aug 15). The Rous sarcoma virus Gag Polyprotein Forms Biomolecular Condensates Driven by Intrinsically-disordered Regions. Journal of molecular biology, 435(16), 168182. . PMID: 37328094.

Description:

The Light Microscopy Imaging Core at Penn State College of Medicine provides consultation and training in ultra-high-resolution imaging of cells and tissues in fixed or live states. The core also provides expertise in quantitative image analysis and consultations on microscopy-related research projects.