Scripps Research Institute
Han Chen
500 University Drive, P.O.Box 850
Room C1730
Hershey, PA 17033
https://research.med.psu.edu/core-facilities/light-microscopy/
RRID: RRID:SCR_022526
Leica SP8 FCS/ FLIM
Light Sheet Fluorescent Microscope (LSFM)
Leica SP8 STED 3X Inverted Confocal
Nikon A1 MP+ Upright Multiphoton
DeltaVisionElite Deconvolution Inverted
Image processing workstation
Confocal Microscope With FCS/FLIM Capabilities
Confocal Microscopy
Confocal/ Super Resolution Microscope
Microscopy
Multiphoton Microscopy
Super-resolution Microscopy
1.) Shaikh K, Bowman M, McCormick SM, Gao L, Zhang J, White J, Tawil J, Kapoor A, Arav-Boger R, Norbury CC, Harhaj EW (2025 Jan 17). ZFAND6 promotes TRAF2-dependent mitophagy to restrain cGAS-STING signaling. iScience, 28(1), 111544. . PMID: 39811672.
2.) Lambert GS, Maldonado RJ, Parent LJ (2025 Jan 13). Role of the Psi Packaging Signal and Dimerization Initiation Sequence in the Organization of Rous Sarcoma Virus Gag-gRNA Co-Condensates Viruses, 17(1), 97. . PMCID: 11769450.
3.) Maldonado RJK, Parent LJ (2025 Jan 11). Dynamic interactions of retroviral Gag condensates with nascent viral RNA at transcriptional burst sites: implications for genomic RNA packaging. bioRxiv : the preprint server for biology, (), . . PMID: 39829876.
4.) White J, Choi YB, Zhang J, Vo MT, He C, Shaikh K, Harhaj EW (2025 Jan). Phosphorylation of the selective autophagy receptor TAX1BP1 by TBK1 and IKBKE/IKKi promotes ATG8-family protein-dependent clearance of MAVS aggregates. Autophagy, 21(1), 160-177. . PMID: 39193925.
5.) Hamamoto K, Liang X, Ito A, Lanza M, Bui V, Zhang J, Opozda DM, Hattori T, Chen L, Haddock D, Imamura F, Wang HG, Takahashi Y (2024 Dec 24). Unveiling the physiological impact of ESCRT-dependent autophagosome closure by targeting the VPS37A ubiquitin E2 variant-like domain. Cell reports, 43(12), 115016. . PMID: 39607828.
6.) Tang M, Dirks K, Kim SY, Qiu Z, Gao Y, Sun D, Peruggia G, Sallavanti J, Li W (2024 Dec 23). Inhibition of thioredoxin reductase 1 sensitizes glucose-starved glioblastoma cells to disulfidptosis. Cell death and differentiation, (), . doi: 10.1038/s41418-024-01440-0. PMID: 39715824.
7.) Bui V, Liang X, Ye Y, Giang W, Tian F, Takahashi Y, Wang HG (2024 Dec 18). Blocking autophagosome closure manifests the roles of mammalian Atg8-family proteins in phagophore formation and expansion during nutrient starvation. Autophagy, (), . doi: 10.1080/15548627.2024.2443300. PMID: 39694607.
8.) Straka J, Khatib JB, Pale L, Nicolae CM, Moldovan GL (2024 Nov 18). CAF-1 promotes efficient PrimPol recruitment to nascent DNA for single-stranded DNA gap formation Nucleic Acids Research, 52(22), 13865-13880. . PMCID: 11662685.
9.) Hanafiah A, Geng Z, Liu T, Tai YT, Cai W, Wang Q, Christensen N, Liu Y, Yue F, Gao Z (2024 Nov 15). PRC1 and CTCF-Mediated Transition from Poised to Active Chromatin Loops Drives Bivalent Gene Activation. bioRxiv : the preprint server for biology, (), . . PMID: 39605346.
10.) Pale LM, Khatib JB, Nusawardhana A, Straka J, Nicolae CM, Moldovan GL (2024 Nov 12). CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single-stranded DNA gaps Nucleic Acids Research, 52(22), 13832-13848. . PMCID: 11662931.
11.) Khatib JB, Dhoonmoon A, Moldovan GL, Nicolae CM (2024 Jul 23). PARP10 promotes the repair of nascent strand DNA gaps through RAD18 mediated translesion synthesis Nature Communications, 15(), 6197. . PMCID: 11266678.
12.) Torres MC, Rebok A, Sun D, Spratt TE (2024 Jul 1). Activity of DNA polymerase κ across the genome in human fibroblasts Proceedings of the National Academy of Sciences of the United States of America, 121(28), e2403130121. . PMCID: 11252913.
13.) Lu T, Yee PP, Chih SY, Tang M, Chen H, Aregawi DG, Glantz MJ, Zacharia BE, Wang HG, Li W (2024 Jul). LC3-associated phagocytosis of neutrophils triggers tumor ferroptotic cell death in glioblastoma. The EMBO journal, 43(13), 2582-2605. . PMID: 38806658.
14.) Khursheed A, Xu LC, Siedlecki CA (2024 Jul). The effects of submicron-textured surface topography on antibiotic efficacy against biofilms. Journal of biomedical materials research. Part B, Applied biomaterials, 112(7), e35436. . PMID: 38961592.
15.) Pale LM, Khatib JB, Nicolae CM, Moldovan GL (2024 Apr 20). CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps bioRxiv, (), 2024.04.17.589988. . PMCID: 11042333.
16.) Ye Y, Liang X, Wang G, Bewley MC, Hamamoto K, Liu X, Flanagan JM, Wang HG, Takahashi Y, Tian F (2024 Mar 15). Identification of membrane curvature sensing motifs essential for VPS37A phagophore recruitment and autophagosome closure Communications Biology, 7(), 334. . PMCID: 10942982.
17.) Lambert GS, Rice BL, Kaddis Maldonado RJ, Chang J, Parent LJ (2024 Jan 18). Comparative analysis of retroviral Gag-host cell interactions: focus on the nuclear interactome bioRxiv, (), 2024.01.18.575255. . PMCID: 10827203.
18.) Kazzaz SA, Shaikh KA, White J, Zhou Q, Powell WH, Harhaj EW (2024 Jan). Phosphorylation of aryl hydrocarbon receptor interacting protein by TBK1 negatively regulates IRF7 and the type I interferon response. The Journal of biological chemistry, 300(1), 105525. . PMID: 38043800.
19.) Saha K, Subramenium Ganapathy A, Wang A, Arumugam P, Michael Morris N, Harris L, Yochum G, Koltun W, Perdew GH, Nighot M, Ma T, Nighot P (2023 Dec). Alpha-tocopherylquinone-mediated activation of the Aryl Hydrocarbon Receptor regulates the production of inflammation-inducing cytokines and ameliorates intestinal inflammation. Mucosal immunology, 16(6), 826-842. . PMID: 37716509.
20.) Chang J, Parent LJ (2023 Nov 22). HIV-1 Gag co-localizes with euchromatin histone marks at the nuclear periphery Journal of Virology, 97(12), e01179-23. . PMCID: 10734548.
21.) Hale A, Dhoonmoon A, Straka J, Nicolae CM, Moldovan GL (2023 Oct 7). Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases. Nature communications, 14(1), 6265. . PMID: 37805499.
22.) Hattori T, Fundora KA, Hamamoto K, Opozda DM, Liang X, Liu X, Zhang J, Uzun Y, Takahashi Y, Wang HG (2023 Sep 21). ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosis Autophagy, 20(2), 349-364. . PMCID: 10813646.
23.) Pandya Shesh B, Slagle-Webb B, Shenoy G, Khristov V, Zacharia BE, Connor JR (2023 Sep). Uptake of H-ferritin by Glioblastoma stem cells and its impact on their invasion capacity. Journal of cancer research and clinical oncology, 149(12), 9691-9703. doi: 10.1007/s00432-023-04864-2. PMID: 37237166.
24.) Kaddis Maldonado R, Lambert GS, Rice BL, Sudol M, Flanagan JM, Parent LJ (2023 Aug 15). The Rous sarcoma virus Gag Polyprotein Forms Biomolecular Condensates Driven by Intrinsically-disordered Regions. Journal of molecular biology, 435(16), 168182. . PMID: 37328094.
25.) Maldonado RK, Rice BL, Lambert GS, Sudol M, Flanagan JM, Parent LJ (2023 Apr 8). The Rous sarcoma virus Gag polyprotein forms biomolecular condensates driven by intrinsically-disordered regions. bioRxiv : the preprint server for biology, (), . . PMID: 37066255.
26.) Yoshioka NK, Young GM, Khajuria DK, Karuppagounder V, Pinamont WJ, Fanburg-Smith JC, Abraham T, Elbarbary RA, Kamal F (2022 Jun 1). Structural changes in the collagen network of joint tissues in late stages of murine OA. Scientific reports, 12(1), 9159. . PMID: 35650306.
27.) Wei Y, Luo H, Yee PP, Zhang L, Liu Z, Zheng H, Zhang L, Anderson B, Tang M, Huang S, Li W (2021 Dec). Paraspeckle Protein NONO Promotes TAZ Phase Separation in the Nucleus to Drive the Oncogenic Transcriptional Program. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8(24), e2102653. . PMID: 34716691.
28.) Hattori T, Takahashi Y, Chen L, Tang Z, Wills CA, Liang X, Wang HG (2021 Feb). Targeting the ESCRT-III component CHMP2A for noncanonical Caspase-8 activation on autophagosomal membranes. Cell death and differentiation, 28(2), 657-670. . PMID: 32807832.
29.) Grillo SL, Etzel JD, Weber SR, Ondeck C, Wang W, Zhao Y, Barber AJ, Sundstrom JM (2021 Feb). Descriptive analysis of Fibulin-3 and the extracellular vesicle marker, Alix, in drusen from a small cohort of postmortem human eyes. Experimental eye research, 203(), 108422. doi: 10.1016/j.exer.2020.108422. PMID: 33387484.
The Light Microscopy Imaging Core at Penn State College of Medicine provides consultation and training in ultra-high-resolution imaging of cells and tissues in fixed or live states. The core also provides expertise in quantitative image analysis and consultations on microscopy-related research projects.
