University of Pennsylvania

Contact Info:

Wenli Yang

https://www.med.upenn.edu/ipsccore/

Grants and Identifiers:

RRID: RRID:SCR_022426

Instrumentation:

Services Provided:

Relevant Publications:

1.) Nirgude S, Tichy ED, Liu Z, Kavari SL, Pradieu RD, Byrne M, Yang F, Gil-de-Gómez L, Mamou B, Bernt KM, Yang W, MacFarland S, Xie M, Kalish JM (2025 Mar 26). Single-nucleus multiomic analysis of Beckwith-Wiedemann syndrome liver reveals PPARA signaling enrichment and metabolic dysfunction. Communications biology, 8(1), 495. . PMCID: 11937391.

2.) Das S, Hilman MC, Yang F, Mourkioti F, Yang W, Cullen DK (2024 Mar 7). Motor neurons and endothelial cells additively promote development and fusion of human iPSC-derived skeletal myocytes Skeletal Muscle, 14(), 5. . PMCID: 10921694.

3.) Pahl MC, Liu L, Pippin JA, Wagley Y, Boehm K, Hankenson KD, Wells AD, Yang W, Grant SFA (2024 Mar). Variant to gene mapping for carpal tunnel syndrome risk loci implicates skeletal muscle regulatory elements. EBioMedicine, 101(), 105038. . PMCID: 10909706.

Description:

Provides resources for disseminating human pluripotent stem cell technology within UPenn and surrounding research communities.  Our services include derivation of patient specific iPSCs, genome engineering of stem cell lines using CRISPR/Cas9 technology, and lineage specific differentiation of iPSCs/hESCs in 2D and 3D organoid culture. Core has large collection of patient derived iPSC lines and cell lines of gastrointestinal tract (esophageal, pancreatic and intestinal) available to users. Committed to training investigators in stem cell culture techniques and providing quality tested stem cell reagents to users.