University of Pennsylvania
Vera Moiseenkova-Bell
https://www.med.upenn.edu/electronmicroscopyresourcelab/
RRID: RRID:SCR_022375
1.) McVeigh BM, De Jesús-Pérez JJ, Siepe DH, Gogoi P, Mageswaran SK, Kalocsay M, Mihelc EM, Moiseenkova-Bell VY (2025 Oct 17). Visualization of lysosomal membrane proteins by cryo electron tomography Nature Communications, 16(), 9234. . PMCID: 12534608.
2.) Yount TA, Porsch EA, St. Geme JW III (2025 Oct 13). Multi-functional minor pilins coordinate type IV pilus assembly, adherence, motility, and DNA uptake in the pediatric pathogen Kingella kingae mBio, 16(11), e02790-25. . PMCID: 12607826.
3.) Shimogawa M, Li MH, Park GSH, Ramirez J, Lee H, Watson PR, Sharma S, Lin Z, Peng C, Lee VM, Garcia BA, Christianson DW, Rhoades E, Eliezer D, Petersson EJ (2025 Aug 22). Investigation of All Disease-Relevant Lysine Acetylation Sites in α-Synuclein Enabled by Non-canonical Amino Acid Mutagenesis. bioRxiv : the preprint server for biology, (), . . PMCID: 11785115.
4.) Trigg NA, Lee GS, Leach AG, Conine CC (2025 Aug 22). Epididymal extracellular vesicles harbor and convey mRNA to sperm for transfer to zygotes bioRxiv, (), 2025.08.18.670952. . PMCID: 12393382.
5.) Eldaboush A, Ricco C, Musante L, Dhiman R, Liu ML, Werth VP (2025 Aug 21). Characterizing Keratinocyte-Derived Extracellular Vesicles in UVB-Irradiated Murine Skin JID Innovations, 6(1), 100406. . PMCID: 12581760.
6.) Shultz RB, Laimo FA, Lee HH, Ali ZS, Huang R, Barnewall RE, Fetzek CG, Ledebur HC, Cullen DK, Katiyar KS (2025 Aug). A Nonhuman Primate Model to Evaluate Treatments for Long-Gap Ulnar Nerve Injury. Journal of neuroscience research, 103(8), e70069. . PMCID: 12836211.
7.) Palumbos SD, Popolow J, Goldsmith J, Holzbaur EL (2025 Jul 7). Autophagic stress activates distinct compensatory secretory pathways in neurons Proceedings of the National Academy of Sciences of the United States of America, 122(28), e2421886122. . PMCID: 12280970.
8.) Wenger ES, Christianson DW (2025 Jun 2). Structure of bifunctional variediene synthase yields unique insight on biosynthetic diterpene assembly and cyclization. Nature communications, 16(1), 5089. . PMCID: 12130500.
9.) Xu Z, Sinha A, Pandya DN, Schnicker NJ, Wadas TJ (2025 May 8). Cryo-electron microscopy reveals a single domain antibody with a unique binding epitope on fibroblast activation protein alpha. RSC chemical biology, 6(5), 780-787. . PMCID: 11834968.
10.) Schultz K, Costa-Pinheiro P, Gardner L, Pinheiro LV, Ramirez-Solis J, Gardner SM, Wellen KE, Marmorstein R (2025 May). Snapshots of acyl carrier protein shuttling in human fatty acid synthase. Nature, 641(8062), 520-528. . PMCID: 12058525.
11.) Chavali SS, Carman PJ, Shuman H, Ostap EM, Sindelar CV (2025 Mar 4). High-resolution structures of Myosin-IC reveal a unique actin-binding orientation, ADP release pathway, and power stroke trajectory. Proceedings of the National Academy of Sciences of the United States of America, 122(9), e2415457122. . PMCID: 11892617.
12.) Han X, Xu Y, Ricciardi A, Xu J, Palanki R, Chowdhary V, Xue L, Gong N, Alameh MG, Peranteau WH, Wilson JM, Weissman D, Mitchell MJ (2025 Mar 1). Plug-and-play assembly of biodegradable ionizable lipids for potent mRNA delivery and gene editing in vivo. bioRxiv : the preprint server for biology, (), . . PMCID: 11888472.
13.) Saks AJ, Barrie KR, Rebowski G, Dominguez R (2025 Feb 25). NPF binding to Arp2 is allosterically linked to the release of ArpC5's N-terminal tail and conformational changes in Arp2/3 complex. Proceedings of the National Academy of Sciences of the United States of America, 122(8), e2421557122. . PMCID: 11873952.
14.) Padilla MS, Mrksich K, Wang Y, Haley RM, Li JJ, Han EL, El-Mayta R, Kim EH, Dias S, Gong N, Teerdhala SV, Han X, Chowdhary V, Xue L, Siddiqui Z, Yamagata HM, Kim D, Yoon IC, Wilson JM, Radhakrishnan R, Mitchell MJ (2025 Jan 24). Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering. Nature communications, 16(1), 996. . PMCID: 11759712.
15.) Yoon IC, Xue L, Chen Q, Liu J, Xu J, Siddiqui Z, Kim D, Chen B, Shi Q, Laura Han E, Cherry Ruiz M, Vining KH, Mitchell MJ (2025 Jan 15). Piperazine-Derived Bisphosphonate-Based Ionizable Lipid Nanoparticles Enhance mRNA Delivery to the Bone Microenvironment. Angewandte Chemie (International ed. in English), 64(3), e202415389. . PMCID: 11735871.
16.) Fenton AR, Peng R, Bond C, Hugelier S, Lakadamyali M, Chang YW, Holzbaur EL, Jongens TA (2024 Nov 15). FMRP regulates MFF translation to locally direct mitochondrial fission in neurons Nature Cell Biology, 26(12), 2061-2074. . PMCID: 11628401.
17.) Ren B, Haase R, Patray S, Nguyen Q, Maco B, Dos Santos Pacheco N, Chang YW, Soldati-Favre D (2024 Nov 8). Architecture of the Toxoplasma gondii apical polar ring and its role in gliding motility and invasion Proceedings of the National Academy of Sciences of the United States of America, 121(46), e2416602121. . PMCID: 11573658.
18.) Wenger ES, Schultz K, Marmorstein R, Christianson DW (2024 Oct 8). Engineering substrate channeling in a bifunctional terpene synthase. Proceedings of the National Academy of Sciences of the United States of America, 121(41), e2408064121. . PMCID: 11474042.
19.) Cory MB, Li A, Hurley CM, Carman PJ, Pumroy RA, Hostetler ZM, Perez RM, Venkatesh Y, Li X, Gupta K, Petersson EJ, Kohli RM (2024 Oct). The LexA-RecA* structure reveals a cryptic lock-and-key mechanism for SOS activation. Nature structural & molecular biology, 31(10), 1522-1531. . PMCID: 11521096.
20.) Kim HJ, Szurgot MR, van Eeuwen T, Ricketts MD, Basnet P, Zhang AL, Vogt A, Sharmin S, Kaplan CD, Garcia BA, Marmorstein R, Murakami K (2024 Jul 25). Structure of the Hir histone chaperone complex. Molecular cell, 84(14), 2601-2617.e12. . PMCID: 11338637.
21.) Liu X, Jean-Gilles R, Baginski J, Cai C, Yan R, Zhang L, Lance K, van der Loo JC, Davidson BL (2024 Jul 15). Evaluation of a rapid multi-attribute combinatorial high-throughput UV-Vis/DLS/SLS analytical platform for rAAV quantification and characterization Molecular Therapy. Methods & Clinical Development, 32(3), 101298. . PMCID: 11338085.
22.) Vrettos N, Oppelt J, Zoch A, Sgourdou P, Yoshida H, Song B, Fink R, O’Carroll D, Mourelatos Z (2024 Mar 23). MIWI N-terminal arginines orchestrate generation of functional pachytene piRNAs and spermiogenesis Nucleic Acids Research, 52(11), 6558-6570. . PMCID: 11194079.
23.) Gaynes MN, Ronnebaum TA, Schultz K, Faylo JL, Marmorstein R, Christianson DW (2024 Mar). Structure of the prenyltransferase in bifunctional copalyl diphosphate synthase from Penicillium fellutanum reveals an open hexamer conformation. Journal of structural biology, 216(1), 108060. . PMCID: 10939776.
Research core is organized into three interdependent facilities. Ultrastructure lab offers training to Standard-EM and Immuno-EM for cellular ultra-structures of tissue samples. Cryo-EM Screening and Training offers training on Microscopes and ancillary equipment for Negative Stain Imaging, Single-Particle Cryo-EM and Cryo-Tomography of large complexes and cells. Beckman Center for Cryo-EM offers service for high resolution data collection of prescreened and well qualified samples for single particle analysis and Tomography using Titan Krios microscope with Gatan K3 camera.
