University of Pennsylvania
Derek Jones
297 John Morgan Building
3620 Hamilton Walk
Philadelphia, PA 19104
https://www.med.upenn.edu/cytomics/
RRID: RRID:SCR_022376
Cell Sorting
Consultation
Experimental Design
FACS Cell Sorting
Flow Cytometric Analysis
Flow Cytometry
Flow Cytometry Data Analysis
High Dimensional Flow Cytometry Analysis
Sample Preparation
Single-Cell Analysis
Spectral Flow Cytometry
Training
1.) Skuli SJ, Bakayoko A, Kruidenier M, Manning B, Pammer P, Salimov A, Riley O, Brake-Sillá G, Dopkin D, Bowman M, Martinez-Gutierrez LN, Anderson CC, Reisz JA, Buono R, Paul M, Saland E, Liccardo F, DeVine A, Wong S, Xu JP, Nee E, Hausler R, Boettcher S, Sebti SM, Lai C, Maxwell KN, Sarry JE, Fruman DA, D’Alessandro A, Mesaros C, Keith B, Simon MC, Sung PJ, Wertheim G, Skuli N, Bowman RL, Matthews A, Carroll M (2025 Jul 9). Chemoresistance of TP53 mutant acute myeloid leukemia requires the mevalonate byproduct, geranylgeranyl pyrophosphate, for induction of an adaptive stress response Leukemia, 39(9), 2087-2098. . PMCID: 12380605.
2.) Murthy A, Rodríguez LR, Barboza WR, Tomer Y, Bui S, Carson P, Dimopoulos T, Iyer S, Chavez K, Cooper CH, Katzen JB, Beers MF (2025 Jul 5). REGULATORY T CELLS PROTECT AGAINST ABERRANT REMODELING IN A MOUSE MODEL OF PULMONARY FIBROSIS bioRxiv, (), 2025.07.02.662777. . PMCID: 12236608.
3.) Rodríguez LR, Alysandratos KD, Katzen J, Murthy A, Roque Barboza W, Tomer Y, Bui S, Acín-Pérez R, Petcherski A, Minakin K, Carson P, Iyer S, Chavez K, Cooper CH, Babu A, Weiner AI, Vaughan AE, Arany Z, Shirihai OS, Kotton DN, Beers MF (2025 Jul 1). Impaired AMPK control of alveolar epithelial cell metabolism promotes pulmonary fibrosis JCI Insight, 10(15), e182578. . PMCID: 12333953.
4.) Ahmadi M, Zhao Z, Dmochowski IJ (2025 Apr 8). RIBOsensor for FRET-based, real-time ribose measurements in live cells Chemical Science, (), . . PMCID: 11977553.
5.) Swingle KL, Hamilton AG, Safford HC, Geisler HC, Thatte AS, Palanki R, Murray AM, Han EL, Mukalel AJ, Han X, Joseph RA, Ghalsasi AA, Alameh MG, Weissman D, Mitchell MJ (2024 Dec 11). Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia Nature, 637(8045), 412-421. . PMCID: 12205896.
6.) Lee CS, Chen S, Berry CT, Kelly AR, Herman PJ, Oh S, O’Connor RS, Payne AS, Ellebrecht CT (2024 Aug 28). Fate induction in CD8 CAR T cells through asymmetric cell division Nature, 633(8030), 670-677. . PMCID: 11410665.
7.) Kadam PS, Yang Z, Lu Y, Zhu H, Atiyas Y, Shah N, Fisher S, Nordgren E, Kim J, Issadore D, Eberwine J (2024 Jul 29). Single-mitochondrion sequencing uncovers distinct mutational patterns and heteroplasmy landscape in mouse astrocytes and neurons BMC Biology, 22(), 162. . PMCID: 11287894.
8.) Kadam PS, Yang Z, Lu Y, Zhu H, Atiyas Y, Shah N, Fisher S, Nordgren E, Kim J, Issadore D, Eberwine J (2024 Jun 19). Single-Mitochondrion Sequencing Uncovers Distinct Mutational Patterns and Heteroplasmy Landscape in Mouse Astrocytes and Neurons bioRxiv, (), 2024.06.13.598906. . PMCID: 11195285.
9.) Denny JE, Alam MZ, Mdluli NV, Maslanka JR, Lieberman LA, Abt MC (2024 May 1). Monoclonal antibody-mediated neutralization of Clostridioides difficile toxin does not diminish induction of the protective innate immune response to infection Anaerobe, 88(), 102859. . PMCID: 11347114.
10.) Wong KG, Cheng YC, Wu VH, Kiseleva AA, Li J, Poleshko A, Smith CL, Epstein JA (2024 Mar 13). Growth factor–induced activation of MSK2 leads to phosphorylation of H3K9me2S10 and corresponding changes in gene expression Science Advances, 10(11), eadm9518. . PMCID: 10936876.
11.) Goldman N, Chandra A, Johnson I, Sullivan MA, Patil AR, Vanderbeck A, Jay A, Zhou Y, Ferrari EK, Mayne L, Aguilan J, Xue HH, Faryabi RB, Wherry EJ, Sidoli S, Maillard I, Vahedi G (2023 Aug 17). Intrinsically disordered domain of transcription factor TCF-1 is required for T cell developmental fidelity Nature immunology, 24(10), 1698-1710. . PMCID: 10919931.
12.) Omo-Lamai S, Zamora ME, Patel MN, Wu J, Nong J, Wang Z, Peshkova A, Chase LS, Essien EO, Muzykantov V, Marcos-Contreras O, Myerson JW, Brenner JS (2023 Jul 25). Physicochemical Targeting of Lipid Nanoparticles to the Lungs Induces Clotting: Mechanisms and Solutions bioRxiv, (), 2023.07.21.550080. . PMCID: 10401951.
13.) Antony C, Somers P, Gray EM, Pimkin M, Paralkar VR (2023 Jul 22). FISH-Flow to quantify nascent and mature ribosomal RNA in mouse and human cells STAR Protocols, 4(3), 102463. . PMCID: 10374870.
14.) Egan KP, Awasthi S, Tebaldi G, Hook LM, Naughton AM, Fowler BT, Beattie M, Alameh MG, Weissman D, Cohen GH, Friedman HM (2023 Jun 30). A Trivalent HSV-2 gC2, gD2, gE2 Nucleoside-Modified mRNA-LNP Vaccine Provides Outstanding Protection in Mice against Genital and Non-Genital HSV-1 Infection, Comparable to the Same Antigens Derived from HSV-1 Viruses, 15(7), 1483. . PMCID: 10384700.
Penn Cytomics & Cell Sorting Facility is the sole flow cytometry shared resource laboratory (SRL) at the University of Pennsylvania. The core has 35+ instruments, which include analyzers, cell sorters, and small particle detectors, as well as a dual-fluorescence cell counter/viability instrument and a tissue dissociator for cell preparation. The SRL staff provides both on-site and off-site support to instrument users, including analyzer and cell sorter training. The facility also has a research and development team that collaborates/consults with principal investigators in developing high-dimensional panels, as well as staining, acquisition, and analysis. The core has ~10 nearby satellite locations to accommodate instrument user needs.
