University of Pennsylvania
Eline T. Luning Prak
410-412 Stellar Chance Labs
422 Curie Blvd.
Philadelphia, PA 19104
https://pathbio.med.upenn.edu/hic/site/
RRID: RRID:SCR_022380
Antigen-Specific T Cell Assays Via ELISPOT Or IC
BCR Sequencing
Blood Cell Products
Blood Processing
Chemiluminescence Immunoassays
Consultation For Pre-experiment Methods, Post-processing Orientation, And/or Special Staining
Data Processing And Analysis
ELISA
Flow Cytometry
Multiplex Assay
Multiplex Cytokine Immunoassays
PBMC Sample Processing And Inventory Management
Protocol Development/Clinical Trial Coordination
Serology Testing
Single Cell Data Analysis
Support For Data Processing And Analysis.
TCR Sequencing
Tissue Processing
1.) Teran Pumar OY, VanNoy EL, Haffey A, Gannamedi DP, Rafie CI, Lykke Harwood DS, Benedetti JR, Pittman Ballard CA, Ciervo E, Assenza Tavares Coroa PH, Grover P, León BE, Mitchell J, Pathak A, Colon B, El Ghorayeb L, O'Sullivan L, Venkatarame Gowda Saralamma V, Lopez Ruiz C, Khatwani N, Kumar S, Rai P, Schatz J, Shah A, Binder ZA, Ceccarelli M, Ostrom QT, Kristensen BW, Stelekati E, Watson DC, Lombard DB, Haydar D, Bayik D (2026 Apr 3). Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activity. bioRxiv : the preprint server for biology, (), . . PMCID: 13060269.
2.) Mao X, Konigsberg J, Noorchashm N, Meng W, Knox JJ, Golden GJ, Hamilton JT, Maxwell TK, Liu C, Betts MR, Willi SM, Naji A, Hanley P, Luning Prak ET (2026 Apr). Differential Contributions of IgM and IgG Autoantibodies to Serologic IA2 Reactivity in Type 1 Diabetes. Biomolecules, 16(4), . . PMCID: 13113336.
3.) Weissman S, Ginda AM, Oceguera A, Swiggard L, Pinzone M, Cannon L, Zurakowski R, Migueles S, Bushman FD, O'Doherty U (2026 Mar 18). Clonal dynamics of HIV-infected and uninfected T cells. EBioMedicine, 126(), 106163. . PMCID: 13015742.
4.) Liu Y, Zhang Z, Tao Y, Rahgav L, Gray-Gaillard S, Hussaini Y, Pan M, Shamber J, Kwak J, Park SL, Cramer J, Stoltz R, Patria J, Swanger J, Liu K, Sannigrahi MK, Houghton AM, Rodriguez CP, Carey RM, Brody R, Rajasekaran K, Weinstein G, Linette GP, Carreno BM, Painter MM, Wherry EJ, Clurman B, Basu D, Diab A (2026 Mar 17). Therapeutic scheduling of WEE1 inhibition preserves T cell function and promotes immune control of HPV⁺ tumors. bioRxiv : the preprint server for biology, (), . . PMCID: 13015432.
5.) McCuaig S, Elliott E, Anderson S, Smith D, Rood J, Gaines J, Kreiger PA, Behrens EM (2026 Mar 15). Damaged glomeruli in proliferative pediatric lupus nephritis exhibit a C5a-C5aR1 induced fibrotic transcriptional program. bioRxiv : the preprint server for biology, (), . . PMCID: 13061038.
6.) Riley J, Deng M, Sinha T, Lai M, Jiang S, Kong H, Cooper V, Gu M, Perelman J, Zhang C, Hanindya C, Carpio V, Keller P, Jadlowsky J, Leskowitz R, McKenna S, Four M, Barranco J, Cooper N, Jain A, Gohil M, Haines K, Gerique L, Russell A, Kulikovskaya I, Gonzalez V, Fraietta J, Plesa G, Sheppard N, Davis M, Chew A, Frey N, June C, Svoboda J, Hexner E (2026 Feb 4). Cell-derived Nanoparticles Provide a Robust Platform to Manufacture Therapeutic T cells. Research square, (), . . PMCID: 12889848.
7.) Haggadone MD, Goldspiel BP, O'Farrell A, Kiledjian NT, Knight M, Smith TR, Anderson E, Vázquez Marrero VR, Boyer MA, Xu PJ, Scaglione M, Powers ZM, Queriault C, Wu A, Yang Q, O'Riordan MX, Raj A, Mesaros C, Conn CS, Shin S, Bailis W (2026 Jan 20). Environmental Amino Acid Sensing Regulates the Rate of ASC Translation and NLRP3 Inflammasome Assembly. bioRxiv : the preprint server for biology, (), . . PMCID: 12871716.
8.) Yang SJ, Lin AA, Shen H, Pappalardo LW, Spychalski GB, Rosario J, Forsberg LK, Grant KM, Buser JR, Savica R, O’Bryant S, Jones DT, Dickson DW, Reichard RR, Nguyen AT, Meaney DF, Boeve BF, Ross OA, McLean PJ, Issadore D (2026). Microfluidic nanomagnetically isolated neuron- and astrocyte-derived extracellular vesicles to differentiate Lewy body and Alzheimer’s disease. Npj Biosensing, 3(1), . . PMCID: 12982126.
9.) Delcy SAS, Farrugia A, Diaz Nieves IA, O'Brien CA, Bennett FC, Cohen AS (2026 Jan). Microglia depletion improves hippocampal circuit function after mild traumatic brain injury in male mice. Brain, behavior, and immunity, 131(), 106178. . PMCID: 13097049.
10.) Sharma N, Sarkar S, Ko T, Edwards KJ, Pham JM, Nguyen T, Gong AZ, Flores J, Trauner D, Sellmyer MA (2025 Dec 27). Photocontrolled trimethoprim PROTACs targeting the eDHFR protein tag Nature Communications, 17(), 822. . PMCID: 12824167.
11.) Malone MJ, Huang C, Zhang Y, Qi Y, Williams L, Su LF, Lou J, Jiang N (2025 Dec 18). Resistance potential of the HLA-A2-restricted immunodominant SARS-CoV-2-specific CD8(+) T cell receptor repertoire to antigenic drift. Nature communications, 17(1), 686. . PMCID: 12820262.
12.) Dubensky SB, Zhu Y, Gallagher M, Kumashie KG, Lu T, Tedesco J, De Luna N, Premo K, Qi Y, Rachimi S, Cabrera EC, Fulmer B, Meremikwu IC, Carter A, Henrickson SE, Romberg N, Baxter AE, Oldridge DA, Vella LA (2025 Dec 13). Multimodal analysis defines GNG4 as a distinguishing feature of germinal center-positioned CD4 T follicular helper cells in humans bioRxiv, (), 2025.12.10.693235. . PMCID: 12713147.
13.) Ma C, Wang H, Liu L, Chen R, Mukherjee N, Tong J, Kazmi S, Fang X, Witkowski MT, Aifantis I, Ghassemi S, Chen W (2025 Dec). Bioengineered immunocompetent preclinical trial-on-chip tool enables screening of CAR T cell therapy for leukaemia. Nature biomedical engineering, 9(12), 2098-2114. . PMCID: 12705464.
14.) Giudice AM, Matlaga S, Roth SL, Pascual-Pasto G, Schürch PM, Rouin G, McIntyre B, Grothusen GP, Cresswell-Clay E, Shraim R, Groff D, Zecchino V, Lombardi S, Martinez D, Spruce LA, Brown EM, Fazelinia H, Henrickson SE, Nance J, Reynolds CP, Bosse KR (2025 Nov 19). Target antigen-displaying extracellular vesicles boost CAR T cell efficacy in cell and mouse models of neuroblastoma. Science translational medicine, 17(825), eads4214. . PMCID: 12958146.
15.) Kulkarni M, Bourne CM, Mahale AB, Exconde PM, Murphy C, Cervantes S, Kardhashi M, Kambayashi M, Yoo W, Wrong TJ, Patio RC, Discher BM, Taabazuing CY (2025 Nov 8). Human non-canonical inflammasomes activate CASP3 to limit intracellular Salmonella replication in macrophages. bioRxiv : the preprint server for biology, (), . . PMCID: 12637516.
16.) Karakasheva TA, Martinez CM, Zhou Y, Qui J, Chen XE, Soto GE, Nettleford SK, Hix OT, Roach DM, Laguerta AM, Thadi A, Edwards RM, Aleynick D, Weinbrom S, Borodyanskaya E, Pickering OH, Fulton M, Chen CH, Peterson IV, Hagen EB, Yannuzzi IP, Haider Z, Cramer Z, Conrad MA, Li N, Bewtra M, Uzun Y, Tan K, Kelsen JR, Minn AJ, Lengner CJ, Hamilton KE (2025 Oct 20). An Epigenetic Basis for Sustained Inflammatory Epithelial Progenitor Cell States in Crohn’s Disease Cellular and Molecular Gastroenterology and Hepatology, 20(3), 101665. . PMCID: 12721313.
17.) Melamed JR, Muscat-Rivera J, Kegel M, Chaboub LS, Perez-Tremble R, Bhalla NS, Ni H, Sun H, Weissman D (2025 Oct 17). Anionic lipids modulate mRNA-lipid nanoparticle immunogenicity and confer protection in a mouse model of multiple sclerosis. bioRxiv : the preprint server for biology, (), . . PMCID: 12632872.
18.) Pastorio C, Richard K, Usmani S, Kissmann AK, Bolotnikov G, Gosálbez G, Hayn M, Koepke L, Sauertnik A, Preising A, Preising N, Ständker L, Fair M, Morris J, Papasavvas E, Liu Q, Sun H, Rodríguez A, Mounzer K, Wiese S, Tebas P, Du Y, Laird GM, Jaritz M, Rosenau F, Gaidt MM, Sparrer KMJ, Montaner LJ, Kirchhoff F (2025 Oct 3). Retinol Binding Protein 4 reactivates latent HIV-1 by triggering canonical NF-κB, JAK/STAT5 and JNK signalling. Signal transduction and targeted therapy, 10(1), 326. . PMCID: 12491451.
19.) Panthi A, Ferretti MB, Howard O, Pokharel SM, McCracken R, Boudreault S, Quesnel-Vallieres M, Li Q, Cherry S, Lynch KW (2025 Sep 23). Alternatively spliced isoforms of IRF7 differentially regulate interferon expression to tune response to viral infection. Cell reports, 44(9), 116166. . PMCID: 12401477.
20.) Goldberg DC, Cloud C, Lee SM, Barnes B, Gruber S, Kim E, Pottekat A, Westphal MS, McAuliffe L, Majounie E, Kalayil Manian M, Zhu Q, Tran C, Hansen M, Stojakovic J, Parker JB, Kohli RM, Porecha R, Renke N, Zhou W (2025 Sep 10). Scalable screening of ternary-code DNA methylation dynamics associated with human traits. Cell genomics, 5(9), 100929. . PMCID: 12534708.
21.) Vadovics M, Zhao W, Daley EF, Lam K, Daly O, Rashid K, Lee HR, Schreiner P, Lundgreen KA, Gaudette BT, Shuvaev VV, Arguiri E, Muramatsu H, Sárközy A, Mdluli T, Xu J, Han X, De Luna N, Castaño D, Bettini E, Ábrahám E, Lipinszki Z, Carlucci G, Bansode AH, Nguyen K, Le TM, Luu T, Muzykantov VR, Bates P, Allman D, Mitchell MJ, Locci M, Radu CG, Heyes J, Pardi N (2025 Sep). Tailoring the adjuvanticity of lipid nanoparticles by PEG lipid ratio and phospholipid modifications. Nature nanotechnology, 20(9), 1312-1322. . PMCID: 13150451.
22.) McCright SJ, Harding O, Chini J, DeMarco N, Hung LY, Pastore CF, Lu W, Rabinowitz J, Henao-Mejia J, Herbert DR, Young LR, Hill DA (2025 Aug 27). Dietary saturated fatty acids promote lung myeloid cell inflammasome activation and IL-1β-mediated inflammation in mice and humans. Science translational medicine, 17(813), eadp5653. . PMCID: 12866988.
23.) Yang SJ, Lin AA, Shen H, Pappalardo LW, Spychalski GB, Rosario J, Forsberg LK, Grant KM, Savica R, O'Bryant S, Jones DT, Dickson DW, Reichard RR, Nguyen AT, Meaney DF, Boeve BF, Ross OA, McLean PJ, Issadore D (2025 Jun 9). Brain-derived extracellular vesicle microRNAs in Lewy body and Alzheimer's disease. bioRxiv : the preprint server for biology, (), . . PMCID: 12258949.
24.) Zhao S, Chen Y, Bhojnagarwala PS, Livingston C, Jose J, Gao Y, Park DH, Herlyn M, Weiner DB (2025 Jun 8). Targeting IL13Rα2 in melanoma with a bispecific T-cell engager: expression profiling and preclinical evaluation. Journal for immunotherapy of cancer, 13(6), . . PMCID: 12161313.
25.) Shukla D, Gabunia K, McGettigan SE, Patel PR, Christensen S, Fan TJ, Song D, Luo Y, Wang Y, Wang H, Young RM, June CH, Scholler J, Riley JL (2025 Jun 4). CAR binders affect CAR T-cell tonic signaling, durability, and sensitivity to target Cancer immunology research, 13(6), 867-880. . PMCID: 12137003.
26.) Knox JJ, Lee I, Blumberg EA, Rosenfeld AM, Meng W, Liu F, Kearns C, O’Doherty U, Shaked A, Olthoff KM, Luning Prak ET (2025 Jun 3). B-Cell Subset Representation Predicts SARS-CoV-2 Vaccine Response in Solid Organ Transplant Recipients The Journal of Infectious Diseases, 232(3), e466-e475. . PMCID: 12455301.
27.) Denny JE, Flores JN, Mdluli NV, Abt MC (2025 Apr 9). Standard mouse diets lead to differences in severity in infectious and non-infectious colitis. mBio, 16(4), e0330224. . PMCID: 11980566.
28.) Poddar S, Chauvin SD, Archer CH, Qian W, Castillo-Badillo JA, Yin X, Disbennett WM, Miner CA, Holley JA, Naismith TV, Stinson WA, Wei X, Ning Y, Fu J, Ochoa TA, Surve N, Zaver SA, Wodzanowski KA, Balka KR, Venkatraman R, Liu C, Rome K, Bailis W, Shiba Y, Cherry S, Shin S, Semenkovich CF, De Nardo D, Yoh S, Roberson EDO, Chanda SK, Kast DJ, Miner JJ (2025 Mar 20). ArfGAP2 promotes STING proton channel activity, cytokine transit, and autoinflammation. Cell, 188(6), 1605-1622.e26. . PMCID: 11928284.
29.) Cardinale CJ, Liu Y, Kevadia A, Strong A, Watts VJ, Hakonarson H (2025 Mar 5). The ulcerative colitis risk gene adenylyl cyclase 7 restrains the T-helper 2 phenotype and Class II antigen presentation. Journal of Crohn's & colitis, 19(3), . . PMCID: 11920793.
30.) Meshanni JA, Stevenson ER, Zhang D, Sun R, Ona NA, Reagan EK, Abramova E, Guo CJ, Wilkinson M, Baboo I, Yang Y, Pan L, Maurya DS, Percec V, Li Y, Gow A, Weissman D, Atochina-Vasserman EN (2025 Feb 21). Targeted delivery of TGF-β mRNA to murine lung parenchyma using one-component ionizable amphiphilic Janus Dendrimers. Nature communications, 16(1), 1806. . PMCID: 11845678.
31.) Whitaker R, Sung S, Tylek T, Risser GE, O'Brien EM, Chua PE, Li T, Petrie RJ, Han L, Binder-Markey BI, Spiller KL (2025 Feb 13). Effects of injury size on local and systemic immune cell dynamics in volumetric muscle loss. NPJ Regenerative medicine, 10(1), 9. . PMCID: 11822203.
32.) O'Farrell A, Niu Z, Li J, Van Eyndhoven LC, Sarma K, Raj A (2025 Jan 24). Innate Immune Memory is Stimulus Specific. bioRxiv : the preprint server for biology, (), . . PMCID: 11785108.
33.) Golinelli G, Scholler J, Roussel-Gervais A, Šakić A, Ilmjärv S, Song D, Gabunia K, Ji M, Fan TJ, Gupta A, Deshmukh M, Berjis A, Cuoghi Costantini R, Apodaca K, Sheppard NC, Kili S, Dominici M, Alessandrini M, June CH, Levine BL (2025). Multiplex engineering using microRNA-mediated gene silencing in CAR T cells. Frontiers in immunology, 16(), 1647433. . PMCID: 12408270.
34.) Denny JE, Alam MZ, Mdluli NV, Maslanka JR, Lieberman LA, Abt MC (2024 Aug). Monoclonal antibody-mediated neutralization of Clostridioides difficile toxin does not diminish induction of the protective innate immune response to infection. Anaerobe, 88(), 102859. . PMCID: 11347114.
35.) Patel RP, Ghilardi G, Zhang Y, Chiang YH, Xie W, Guruprasad P, Kim KH, Chun I, Angelos MG, Pajarillo R, Hong SJ, Lee YG, Shestova O, Shaw C, Cohen I, Gupta A, Vu T, Qian D, Yang S, Nimmagadda A, Snook AE, Siciliano N, Rotolo A, Inamdar A, Harris J, Ugwuanyi O, Wang M, Carturan A, Paruzzo L, Chen L, Ballard HJ, Blanchard T, Xu C, Abdel-Mohsen M, Gabunia K, Wysocka M, Linette GP, Carreno B, Barrett DM, Teachey DT, Posey AD, Powell DJ Jr, Sauter CT, Pileri S, Pillai V, Scholler J, Rook AH, Schuster (2024 Jul 19). CD5 deletion enhances the antitumor activity of adoptive T cell therapies. Science immunology, 9(97), eadn6509. . PMCID: 11917452.
36.) Metzloff AE, Padilla MS, Gong N, Billingsley MM, Han X, Merolle M, Mai D, Figueroa-Espada CG, Thatte AS, Haley RM, Mukalel AJ, Hamilton AG, Alameh MG, Weissman D, Sheppard NC, June CH, Mitchell MJ (2024 Jun). Antigen Presenting Cell Mimetic Lipid Nanoparticles for Rapid mRNA CAR T Cell Cancer Immunotherapy. Advanced materials (Deerfield Beach, Fla.), 36(26), e2313226. . PMCID: 11209815.
37.) Yu J, Zamani A, Goldsmith JR, Etwebi Z, Lee CN, Chen YH, Sun H (2023 Nov 11). TIPE proteins control directed migration of human T cells by directing GPCR and lipid second messenger signaling Journal of Leukocyte Biology, 115(3), 511-524. . PMCID: 10890839.
The Human Immunology Core (HIC) provides wet bench expertise and infrastructure support for early phase clinical trials and other investigations. The HIC offers online ordering of purified cell subsets from HLA-typed healthy human apheresis donors. HIC staff are internally and externally qualified to perform blood (PBMC) and tissue processing for viable cryopreservation following validated standard operating procedures. The HIC also offers a wide range of immunological assays including digital ELISA, ELISA, ELISPOT, Luminex, flow cytometry and immune repertoire profiling (NGS of BCR and TCR rearrangements in bulk and single cell formats). The HIC offers investigators expertise and guidance in study design, sample processing, regulatory compliance, immunology assay design and validation, data analysis and presentation, and grant writing support.
