University of Pennsylvania

Contact Info:

Eline Luning Prak

https://pathbio.med.upenn.edu/hic/site/

Grants and Identifiers:

RRID: RRID:SCR_022380

Instrumentation:

Services Provided:

Relevant Publications:

1.) Yu J, Zamani A, Goldsmith JR, Etwebi Z, Lee CN, Chen YH, Sun H (2023 Nov 11). TIPE proteins control directed migration of human T cells by directing GPCR and lipid second messenger signaling Journal of Leukocyte Biology, 115(3), 511-524. . PMCID: 10890839.

Description:

The Human Immunology Core (HIC) provides wet bench expertise and infrastructure support for early phase clinical trials and other investigations. The HIC offers online ordering of purified cell subsets from HLA-typed healthy human apheresis donors. HIC staff are internally and externally qualified to perform blood (PBMC) and tissue processing for viable cryopreservation following validated standard operating procedures. The HIC also offers a wide range of immunological assays including digital ELISA, ELISA, ELISPOT, Luminex, flow cytometry and immune repertoire profiling (NGS of BCR and TCR rearrangements in bulk and single cell formats). The HIC offers investigators expertise and guidance in study design, sample processing, regulatory compliance, immunology assay design and validation, data analysis and presentation, and grant writing support.