University of Nebraska Medical Center

Contact Info:

985990 Nebraska Medical Center

Omaha, NE 68198-6805

https://www.unmc.edu/vcr/cores/vcr-cores/small-animal-imaging/miti/index.html

Grants and Identifiers:

RRID: RRID:SCR_022478

P30GM127200 (NIGMS, NCN)

P20GM130447 (NIGMS, CoNDA)

P30CA036727 (NCI, Buffett Cancer Center)

S10OD030486 (NIH)

Nebraska Research Initiative

UNMC Vice Chancellor for Research Office

Instrumentation:

Services Provided:

3D Visualization

Cell Imaging

Confocal Microscopy

Consultation

Consultations

Data Analysis

Data Analysis And Interpretation

Data Visualization

Fluorescence Microscope With Optical Sectioning Capability

Image Aquisition

Image Processing And Analysis

Immunofluorescence

In-vivo Imaging

Live Cell Imaging

Microscopy

Multiphoton Microscopy

Multiphoton/ Harmonic Generation Microscope

Scanning 20X Fluorescent

Training

Relevant Publications:

1.) Kitzerow O, Christensen S, Hong J, Adam RJ, Zucker IH, Jensen‐Smith H, Wang H (2025 Jan 16). Anatomical mapping of neural lineages expressing the transient receptor potential vanilloid type 1 receptor using a modified and combined PACT and CUBIC protocol for rapid tissue clearance Acta Physiologica (Oxford, England), 241(2), e14275. . PMCID: 11737471.

2.) Alsamraae M, Costanzo-Garvey D, Teply BA, Boyle S, Sommerville G, Herbert ZT, Morrissey C, Dafferner AJ, Abdalla MY, Fallet RW, Kielian T, Jensen-Smith H, deOliveira EI, Chen K, Bettencourt IA, Wang JM, McVicar DW, Keeley T, Yu F, Cook LM (2023 Nov 7). Androgen receptor inhibition suppresses anti-tumor neutrophil response against bone metastatic prostate cancer via regulation of TβRI expression Cancer letters, 579(), 216468. . PMCID: 10710875.

3.) Tang W, Panja S, Jogdeo CM, Tang S, Ding L, Yu A, Foster KW, Dsouza DL, Chhonker YS, Jensen-Smith H, Jang HS, Boesen EI, Murry DJ, Padanilam B, Oupický D (2022 Jun). Modified chitosan for effective renal delivery of siRNA to treat acute kidney injury. Biomaterials, 285(), 121562. . ID: 35552115.

4.) Zhang C, Hang Y, Tang W, Sil D, Jensen-Smith HC, Bennett RG, McVicker BL, Oupický D (2022 Mar 18). Dually Active Polycation/miRNA Nanoparticles for the Treatment of Fibrosis in Alcohol-Associated Liver Disease. Pharmaceutics, 14(3), . . ID: 35336043.

Description:

The Multiphoton Intravital & Tissue Imaging (MITI) Core Facility, is part of the Translational Imaging and Behavioral Assessment (TIBA) Core within the Cognitive Neuroscience of Development & Aging (CoNDA) Center and larger Fred & Pamela Buffett Cancer Center Preclinical Imaging Center. The MITI Core is equipped with experienced research professionals and state-of-the-art intravital imaging equipment to acquire advanced descriptive and quantitative data for various in-vivo/preclinical imaging studies. The MITI Core is equipped with an Olympus FVMPE-RS advanced multiphoton microscope, a multichannel, multiphoton excitation system specifically tailored for intravital and deep tissue imaging. It delivers high speed (up to millisecond imaging) for rapid in vivo imaging of physiological responses, near infrared excitation for deep tissue imaging. The Olympus FVMP-RS system is additionally equipped with 458 and 588 nm stimulation laser lines for photobleaching, light stimulation and/or photoactivation of various fluorescent or caged constructs. The Olympus FVMPE-RS Multiphoton Microscope uses a Spectra-Physics InSight X3 dual-line, tunable multiphoton laser for ultrafast, multichannel imaging. The main output from the InSight laser is a tunable line ranging from 680 to 1300 nm with a second multiphoton excitation line at 1045 nm. This extended excitation range supports both traditional fluorophores and fluorescent proteins (blue to long range red), as well as second and third harmonic imaging. The available Neurotar Mobile Home Cage (Funding: P20 GM130447) allows researchers to integrate real-time imaging with behavioral/locomotion tracking. Training and access to the imaging system, support in the design of experimental procedures, acquisition of image data, and analysis of acquired images are offered through the MITI Core. Services are available to investigators within the University of Nebraska network, as well as academic or commercial/industrial institutions in the region at hourly usage rates. The Cognitive Neuroscience of Development and Aging (CoNDA) Center is a NIH Center of Biomedical Research Excellence (COBRE) at the University of Nebraska Medical Center in Omaha, Nebraska, with a focus on neuroscience research across the lifespan. The center aims to advance translational and human neuroscience through support of research projects and development of infrastructure. The Fred and Pamela Buffett Cancer Center Preclinical Imaging Center comprised of the MITI core, Ultrasound and Photoacoustics core, and IVIS core, is recognized by the NIH as a National Cancer Institute (NIH) cancer center shared research resource. The MITI is also supported by the Nebraska Center for Nanomedicine CoBRE at the University of Nebraska Medical Center in Omaha, Nebraska.